A5048133799- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Circadian rhythm and melatonin
- Ovarian function and disorders
- Reproductive Physiology in Livestock
- Seed Germination and Physiology
- Gas Sensing Nanomaterials and Sensors
- Neuroendocrine regulation and behavior
- Genetics, Bioinformatics, and Biomedical Research
- Animal Behavior and Welfare Studies
- Reproductive biology and impacts on aquatic species
- Insect and Pesticide Research
- Ion-surface interactions and analysis
- Coccidia and coccidiosis research
Universidad de Zaragoza
2019-2022
Universitat Autònoma de Barcelona
2016
In the field of assisted reproductive techniques (ART), computer-assisted sperm analysis (CASA) systems have proved their utility and potential for assessing quality, improving prediction fertility a seminal dose. Although most laboratories scientific centers use commercial systems, in recent years certain free open-source alternatives emerged that can reduce costs research groups to face. However, these cannot analyze kinetic responses different stimuli, such as chemotaxis, thermotaxis or...
Reactive oxygen species (ROS) play an essential role in mammalian sperm capacitation. NADPH oxidase 5 (NOX5) has been described as the main source of ROS production some spermatozoa, such human and equine. On other hand, melatonin can decrease cellular levels regulates NOX activity somatic cells. Therefore, objectives this work were (1) to identify NOX5 ram spermatozoa analyze its possible changes during vitro capacitation (2) investigate effect on expression localization superoxide...
Testicular ultrasound is a non-invasive technique that could be very useful for predicting ram seminal quality. Recent software developments allow macroscopic and microscopic evaluation of testicular parenchyma. Thus, the aim this study was to evaluate echotexture using ultrasound-video analysis investigate its possible correlation with semen Nine rams were evaluated one year portable scanner analyzed ECOTEXT® software. The number black (Ec1), white (Ec2), grey pixels (Ec3), tubular density...
Nitric oxide (NO·), synthesized from L-arginine by nitric synthase (NOS), is involved in sperm functionality. NOS isoforms have been detected spermatozoa different species, and an increment activity during capacitation has reported. This work aims to determine the presence localization of ram analyse their possible changes vitro capacitation. Likewise, we investigated effect melatonin on expression NO· levels capacitated spermatozoa. Western blot analysis revealed protein bands associated...
Apart from the canonical cAMP-PKA pathway, ram sperm capacitation can be achieved by MAPK ERK1/2 signalling cascade, activated epidermal growth factor (EGF).This study aims to investigate effect of melatonin and nitric oxide (NO·) on apoptotic-like changes in EGF-capacitated spermatozoa.In vitro was induced EGF absence or presence (100pM 1μM). Also, a NO· precursor, L-arginine, NOS inhibitor, NG-nitro-L-arginine methyl ester (L-NAME), were added media interaction during EGF-capacitation....
Sperm orientation mechanisms, such as chemotaxis, are essential for the sperm to reach oocyte and fertilize it. Melatonin is secreted by cumulus cells also present in follicular fluid mammals. The presence of membrane receptors melatonin ram spermatozoa, its proven involvement functionality, may suggest a possible role guided movement towards oocyte. Hence, objective work study vitro potential chemotactic action on analysing influence season (breeding non-breeding) capacitation state. first...