In the present study, analysis of epithelial cells derived from various sources was undertaken, beginning mammary gland tissue through primary cultures and their subsequent passages. The objective study comparative stage in which obtained individuals different lactation cycles disparate phases cell culture growth are most suitable for morphological research gene expression activity. bovine passages were identified morphologically using immunocytochemical methods. After positive...

Abstract. Associations between polymorphism localised in the third exon of prolactin gene (PRL-RsaI) and milk xproduction traits Black-and-White Jersey cattle were analysed. A total 427 cows included study. PCR-RFLP method was used. The frequencies genotypes alleles as follows: 0.7107 – AA, 0.2851 AB, 0.0042 BB; 0.8533 PRLA 0.1467 PRLB for 0.0919 0.4324 0.4757 0.3081 0.6919 cattle. Statistically significant differences breeds observed alleles. PRL-RsaI production lack associations with these...

Abstract. Associations between polymorphism localised in the six exon of PIT1 gene (PIT1-HinfI) and milk production traits Black-and-White cattle were analysed. A total 900 cows included study. PCR-RFLP method was used. The frequencies genotypes alleles as follows: 0.054 for AA, 0.377 AB 0.569 BB, 0.243 PIT1A 0.757 – PIT1B. There no associations PIT1-HinfI cows.

Objective: The aim of the present study was to compare effectiveness three approaches (the seasonal auto-regressive integrated moving average [SARIMA] model, nonlinear autoregressive exogenous [NARX] artificial neural networks and Wood’s model) prediction milk yield during lactation.Methods: dataset comprised monthly test-day records from 965 Polish Holstein-Friesian Black-and-White primiparous cows. yields cows in their first lactation (from 5 305 days milk) were used. Each divided into ten...

The aim of this study was to relate polymorphic variants CYP19 , CYP21 and ER1 genes reproductive traits in 472 Polish Holstein-Friesian cows. High frequencies one the homozygous genotypes were found. / Sna BI AA homozygotes not identified. In first third lactation, an average calving-to-conception interval (CLVC) cows GG genotype significantly shorter ( P ≤ 0.05) than heterozygous Bgl I genotype, CLVC observed compared heterozygotes whereas longer ones. It also found that characterized by...

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The aim of this study was to apply boosted classification trees dystocia detection in dairy cattle and indicate its most important predictors. A total 1742 calving records were used. Proportions correctly detected difficult easy calvings a test set 75.0%, 92.0% 77.3%, for heifers multiparous cows, respectively. key predictors difficulty were: pregnancy length, body condition score index, age, proportion Holstein-Friesian genes (for heifers) as well calving-to-conception intervals, mean daily...

Abstract Szatkowska, I., Dybus, A., Grzesiak, W., Jedrzejczak, M. and Muszyńska, 2009. Association between the growth hormone releasing (GHRH) gene polymorphism milk production traits of dairy cattle. J. Appl. Anim. Res., 36: 119–123. The association GHRH/HaeIII polymorphism, Polish Holstein Jersey cows were analysed. A total 427 animals included in study. PCR-RFLP method was used for genotyping. frequencies genotypes alleles 0.078—AA, 0.339—AB, 0.583—BB 0.248—GHRHA , 0.752—GHRHB. For cows,...

Evaluation of the applicability polymerase chain reaction (PCR) to sex identification in Eurasian blackbirds ( Turdus merula ) is one most common bird species Europe. This study verified a method for its by PCR with P2/P8 primers, based on CHD1 gene polymorphism, proposed earlier studies as universal tool species. Although there are some reports that cannot determine due lack differences intron lengths CHD1-Z and CHD1-W genes, our showed such possibility does exist, so it can contribute an...

Abstract. Associations between two polymorphisms localized in the seventh exon of butyrophilin gene (BTN/HaeIII and BTN/SchI) milk production traits Jersey cattle were analysed. A total 171 cows included study. PCR-RFLP method was used. In both three genotypes identified. Statistically significant differences polymorphic variants observed. Those concerned percentage fat content case additionally yield protein BTN/HaeIII polymorphism. The influence combined (BTN/HaeIII/SchI) on examined also...

The aim of this study was to compare the expression levels SOX9 (SRY-box 9) gene in testes 18 White improved male goats (Capra hircus) divided into three age groups (one, 10 and 15 months with seven, eight individuals per group, respectively). Abnormalities testis development were observed from group 10-month-olds. Additionally, differences that may affect process maturation testicular spermatogenic activity investigated among individuals. tissues analysed using qPCR. Maximal 10-month-old...

Polledness intersex syndrome (PIS) is a phenomenon associated with 2 distinct conditions occurring in goats, namely polledness and intersexuality. The first trait exhibiting complete dominance, whereas the latter recessive. extreme case of intersexualism sex reversal, which observed only females (60,XX). PIS inherited recessive manner caused by deletion 11.7 kbp DNA on chromosome 1q43. Female goats exhibit broad range developmental abnormalities sexual system, from subtle changes to...

Abstract. The relationship between the SNP of cytochrome P450 gene (CYP19-PvuII) and milk production traits Black-and-White Jersey cattle were analysed. A total 437 cows included in study. PCR-RFLP was used to genotype. frequencies genotypes alleles for as follows: 0.8985 – AA, 0.0977 AB, 0.0038 BB, 0.9474 CYP19A, 0.0526 CYP19B. In Jersey, all genotyped CYP19AA (no polymorphism). There weren’t any associations CYP19-PvuII polymorphism investigated cows.

So far the studies on prion protein (PRNP) gene have mainly been focused search for an association between detected polymorphisms in this and susceptibility of cattle to bovine spongiform encephalopathy. The PRNP location chromosome 13 suggests that within can be potential quantitative trait locus (QTL) markers milk production traits. aim study was analysis effect insertion/deletion promoter region (23 bp) intron 1 (12 traits Jersey cattle. material consisted 171 cows. following were...

The aim of the present study was to illustrate predictive performance random forest (RF) used for dystocia detection in dairy cattle. A total 1,342 and 1,699 calving records Polish Holstein-Friesian Black-and-White heifers cows were used. Five or ten predictor variables included RF models cows, respectively. output variable class. proportion correctly detected easy, moderate difficult events on independent test set 39.64 %, 57.39 % 83.64 accuracy recorded as 60.12 %. The corresponding...