Abstract Xylem embolism is a limiting factor for woody species worldwide. Conifers at the alpine timberline are exposed to drought and freeze-thaw stress during winter, which induce potentially lethal embolism. Previous studies indicated that trees survive by xylem refilling. In this study on Picea abies, refilling was monitored winter spring seasons analyzed in laboratory situ experiments, based hydraulic, anatomical, histochemical methods. Refilling started late when soil frozen water not...

Summary Conifer needles have been reported to absorb water under certain conditions. Radial movement across needle tissues is likely influenced by aquaporin ( AQP ) channels. Foliar uptake and localization in P icea glauca were studied using physiological microscopic methods. expression was measured quantitative real‐time PCR . Members of the gene family spruce identified homology search tools. Needles drought‐stressed plants absorbed when exposed high relative humidity RH ). s present...

Isohydric plants tend to maintain a water potential homeostasis primarily by controlling loss via stomatal conductance. However, there is accumulating evidence that can also modulate uptake in dynamic manner. The dynamics of are influenced aquaporin-mediated changes root hydraulics. Most studies this area have been conducted on herbaceous plants, and less known about responses woody plants. Here study was determine how roots hybrid poplar (Populus trichocarpa×deltoides) respond step change...

Together with longer production periods, the commercial transition to day-neutral strawberry (Fragaria × ananassa) varieties has favored development of diseases such as powdery mildew (Podosphaera aphanis) that thrives in late summer-early fall. In an attempt find alternative solutions fungicides currently employed curb disease, we wanted investigate potential silicon (Si) amendments have been associated prophylactic properties against mildews. To this end, our first objective was determine...

Two C-repeat binding factor genes (EguCBF1a/b), isolated from E. gunnii and differentially cold-regulated, were constitutively overexpressed in a cold-sensitive Eucalyptus hybrid. In addition to the expected improvement on freezing tolerance, some resulting transgenic lines (EguCBF1a-OE EguCBF1b-OE) exhibited decrease stomata density an over-accumulation of anthocyanins also observed lesser extent cold-acclimated control plant. Given that induction five putative CBF target was...

Tritrophic interactions involving a biocontrol agent, pathogen and plant have been analyzed predominantly from the perspective of agent. We conducted first comprehensive transcriptomic analysis all three organisms in an effort to understand elusive properties Pseudozyma flocculosa context its activity against Blumeria graminis f.sp. hordei as it parasitizes Hordeum vulgare. After inoculation P. flocculosa, tripartite interaction was monitored over time samples collected for scanning electron...

ABSTRACT When light levels and evaporative demand increase, dynamic physiological changes in roots may be required to restore the water balance at whole plant level. We hypothesized that a increase root hydraulic conductance ( L P ) aquaporin (AQP) expression could moderate transpiration‐induced drop potential Ψ ), allowing continued gas exchange hybrid poplar Populus trichocarpa × deltoides saplings. Fifty‐six AQP s have been identified poplar, but little information about their patterns is...

Gas exchange is constrained by the whole-plant hydraulic conductance (Kplant). Leaves account for an important fraction of Kplant and may therefore represent a major determinant plant productivity. Leaf (Kleaf) decreases with increasing water stress, which due to xylem embolism in leaf veins and/or properties extra-xylary pathway. Water flow through living tissues facilitated regulated channel proteins called aquaporins (AQPs). Here we assessed changes Populus trichocarpa leaves during...

Aquaporin membrane water channels have been previously identified in the phloem of angiosperms, but currently their cellular characterization is lacking, especially tree species. Pinpointing location will help generate new hypotheses how exchange facilitates sugar transport plants.We studied histological sections balsam poplar (Populus balsamifera L.) leaf, petiole, and stem organs. Immuno-labeling techniques were used to characterize distribution PIP1 PIP2 subfamilies aquaporins along...

Abstract The herbicide glyphosate has several potential entry points into composting sites and its impact on processes not yet been evaluated. To assess bacterial diversity abundance as well community composition dynamics, we conducted a mesocosm experiment at the Montreal Botanical Garden. Glyphosate had no effect physicochemical property evolution during composting, while it was completely dissipated by end of experiment. Sampling Days 0, 2, 28 112 process followed 16S rRNA amplicon...

White spruce (Picea glauca (Moench) Voss) is a commercially valuable boreal tree that has been known for its ability to colonize deglaciated rock tailings. Over the last decade, there an increasing interest in using this species revegetation and successful restoration of abandoned mine spoils. Herein, we conducted glasshouse experiment screen mycorrhizal fungi rhizobacteria capable improving health growth white seedlings growing directly on waste rocks or fine tailings from Sigma-Lamaque...

In the context of a phytorestoration project, purpose this study was to assess respective contribution nutritional status Picea glauca seedlings ectomycorrhizae and rhizobacteria native or not Sigma-Lamaque gold mine wastes in northern Quebec, Canada. glasshouse experiment, inoculated plants were grown for 32 weeks on coarse waste rocks fine tailings obtained from mining site. The survival, health, growth better than tailings. Fe Ca especially found at high levels plant tissues but much...

Phytoremediation shows great promise as a plant-based alternative to conventional clean-up methods that are prohibitively expensive. As part of an integrated strategy, the selection well-adapted plant species well planting and management techniques could determine success long-term program. Herein, we conducted experiment under semi-controlled conditions screen different plants with respect their ability phytoremediate Zn-contaminated soil excavated from contaminated site following train...

Traditional treatment of wastewaters is a burden for local governments. Using short rotation coppice willow (SRCW) as vegetal filter has several environmental and economic benefits. Here, we investigated the effect primary wastewater irrigation on wood structure composition cultivar Salix miyabeana 'SX67' following two years growth. Compared to unirrigated plants (UI), stem sections irrigated with (WWD) showed an unexpected decrease hydraulic conductance (KS) associated in vessel density but...

Previous experiments using heat exchangers (liquid cooled blocks) to chill a portion of plant stem have shown transient stoppage in phloem translocation and an increase measured pressure. Although chilled-induced transport has been known for over 100 years, the mechanism this phenomenon is still poorly understood. Recently, work highlighted that aquaporins occur within plasma membrane sieve tubes along entire source-to-sink pathway, isoforms these water channel proteins may change...