A5097954848- Enzyme Structure and Function
- Protein Structure and Dynamics
- Heat shock proteins research
- Molecular spectroscopy and chirality
- Mass Spectrometry Techniques and Applications
Utrecht University
2022-2024
Center for Integrated Protein Science Munich
2022
Technical University of Munich
2022
ABSTRACT Biomolecular structures are typically determined using frozen or crystalline samples. Measurement of intramolecular distances in solution can provide additional insights into conformational heterogeneity and dynamics biological macromolecules their complexes. The established molecular ruler techniques used for this (NMR, FRET, EPR) are, however, limited dynamic range require model assumptions to determine absolute distance (distributions). Here, we introduce anomalous X-ray...
We establish high-resolution (<1 Å) intramolecular distance measurements in proteins using pairs of small (1.4 nm diameter) gold nanoparticle labels and anomalous small-angle X-ray scattering as a read out.