A5042381556- Neuroinflammation and Neurodegeneration Mechanisms
- Mast cells and histamine
- Alzheimer's disease research and treatments
- Cholesterol and Lipid Metabolism
- Computational Drug Discovery Methods
- Sphingolipid Metabolism and Signaling
- Medicinal Plants and Bioactive Compounds
- Complement system in diseases
- Glaucoma and retinal disorders
- Immune cells in cancer
- Mitochondrial Function and Pathology
- Retinal Diseases and Treatments
- Olfactory and Sensory Function Studies
- FOXO transcription factor regulation
- Eosinophilic Disorders and Syndromes
- Biochemical Acid Research Studies
- Reproductive System and Pregnancy
- melanin and skin pigmentation
- Axon Guidance and Neuronal Signaling
- RNA regulation and disease
- Angiogenesis and VEGF in Cancer
- Retinal Development and Disorders
- Genetics, Aging, and Longevity in Model Organisms
- Ocular Surface and Contact Lens
- Barrier Structure and Function Studies
Baylor College of Medicine
2020-2024
Johns Hopkins University
2016-2018
Johns Hopkins Medicine
2016-2018
Johns Hopkins Hospital
2017
Microglia are the major cell type expressing complement C3a receptor (C3aR) in brain. Using a knockin mouse line which Td-tomato reporter is incorporated into endogenous C3ar1 locus, we identified 2 subpopulations of microglia with differential C3aR expression. Expressing on APPNL-G-F-knockin (APP-KI) background revealed significant shift to high-C3aR-expressing subpopulation and they were enriched around amyloid β (Aβ) plaques. Transcriptomic analysis C3aR-positive documented dysfunctional...
Abstract Background Age is the strongest risk factor for development of Alzheimer’s disease (AD). Besides pathological hallmarks β-amyloid (Aβ) plaques and neurofibrillary tangles, emerging evidence demonstrates a critical role microglia neuroinflammation in AD pathogenesis. Oleoylethanolamide (OEA) an endogenous lipid amide that has been shown to promote lifespan healthspan C. elegans through regulation lysosome-to-nucleus signaling cellular metabolism. The goal our study was determine OEA...
Topical medications that inhibit the enzyme carbonic anhydrase (CAI) are widely used to lower intraocular pressure in glaucoma; however, their clinical efficacy is limited by requirement for multiple-daily dosing, as well side effects such blurred vision and discomfort on drop instillation. We developed a biodegradable polymer microparticle formulation of CAI dorzolamide produces sustained lowering after subconjunctival injection. Dorzolamide was ion paired with sodium dodecyl sulfate (SDS)...
Abstract The rise of life expectancy the human population is accompanied by drastic increases age‐associated diseases, in particular Alzheimer's disease (AD), and underscores need to understand how aging influences AD development. Forkhead box O transcription factor 3 (FoxO3) known mediate longevity downstream insulin/insulin‐like growth signaling across species. However, its function adult brain under physiological pathological conditions less understood. Here, we report a region...
Purpose: Our previous study demonstrated significantly more degranulating mast cells (MCs) in choroids from subjects with age-related macular degeneration compared to aged controls. This examined the immunolocalization of tryptase, most abundant MC secretory granule-derived serine protease, control eyes and geographic atrophy (GA). Methods: Postmortem human without GA were obtained National Disease Research Interchange. Tissue was fixed, cryopreserved, sectioned, immunostained a monoclonal...
Abstract Emerging evidence implicates impaired microglia function and dysregulation of lipid metabolism in Alzheimer’s disease (AD). Oleoylethanolamide (OEA), an endogenous PPARα agonist, has been shown to promote longevity C. elegans through regulation lysosome-to-nucleus signaling cellular metabolism. Using a stable OEA analog, KDS-5104, we found that OEA-PPARα promotes TFEB lysosomal activity independent mTORC1 upregulates cell-surface receptor CD36, leading enhanced microglial Aβ uptake...