A5027724470- Mitochondrial Function and Pathology
- ATP Synthase and ATPases Research
- RNA and protein synthesis mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Advanced Proteomics Techniques and Applications
- Ubiquitin and proteasome pathways
- DNA and Nucleic Acid Chemistry
- Fungal and yeast genetics research
- Biotin and Related Studies
- Trypanosoma species research and implications
- Bacteriophages and microbial interactions
University of Freiburg
2017-2025
University of Wisconsin–Madison
2024-2025
Quantitative BioSciences
2024
Mitochondria are key organelles for cellular energetics, metabolism, signaling, and quality control have been linked to various diseases. Different views exist on the composition of human mitochondrial proteome. We classified >8,000 proteins in preparations cells defined a high-confidence proteome >1,100 (MitoCoP). identified interactors translocases, respiratory chain, ATP synthase assembly factors. The abundance MitoCoP covers six orders magnitude amounts 7% with chaperones HSP60-HSP10...
Abstract Protein import into organelles is essential for all eukaryotes and facilitated by multi-protein translocation machineries. Analysing whether a protein transported an organelle largely restricted to single constituents. This renders knowledge about imported proteins incomplete, limiting our understanding of organellar biogenesis function. Here we introduce method that enables charting organelle’s importome. The approach relies on inducible RNAi-mediated knockdown subunit translocase...
The mitochondrial proteome arises from dual genetic origin. Nuclear-encoded proteins need to be transported across or inserted into two distinguished membranes, and the TOM complex represents main translocase in outer membrane. Its composition regulations have been extensively investigated within yeast cells. However, we little knowledge of human Here, defined interactome a comprehensive manner using biochemical approaches isolate combination with quantitative mass spectrometry analyses....
Abstract G-quadruplex (G4) structures can form in guanine-rich DNA or RNA and have been found to modulate cellular processes, including replication, transcription, translation. Many studies on the roles of G4s focused eukaryotic systems, with far fewer probing bacterial G4s. Using a chemical-genetic approach, we identified genes Escherichia coli that are important for growth G4-stabilizing conditions. Reducing levels translation elongation factor Tu slowing initiation kasugamycin,...
The F1F0-ATP synthase translates a proton flux across the inner mitochondrial membrane into mechanical rotation, driving anhydride bond formation in catalytic portion. complex's membrane-embedded motor forms proteinaceous channel at interface between Atp9 ring and Atp6. To prevent unrestricted flow dissipating H+-gradient, is critical tightly controlled step during ATP assembly. Here we show that INA complex (INAC) acts this decisive promoting Atp9-ring association with INAC binds to newly...
Knowledge about the functions of individual proteins on a system-wide level is crucial to fully understand molecular mechanisms underlying cellular processes. A considerable part proteome across all organisms still poorly characterized. Mass spectrometry an efficient technology for global study proteins. One most prominent methods accurate proteome-wide comparative quantification stable isotope labeling by amino acids in cell culture (SILAC). However, application SILAC prototrophic such as...
Abstract G-quadruplex (G4) structures can form in guanine-rich DNA or RNA and have been found to modulate cellular processes including replication, transcription, translation. Many studies on the roles of G4s focused eukaryotic systems, with far fewer probing bacterial G4s. Using a chemical-genetic approach, we identified genes Escherichia coli that are important for growth G4-stabilizing conditions. Reducing levels elongation factor Tu slowing translation chloramphenicol suppress effects G4...