A5054104128- Privacy-Preserving Technologies in Data
- Erythrocyte Function and Pathophysiology
- Nitric Oxide and Endothelin Effects
- Privacy, Security, and Data Protection
- Ion Transport and Channel Regulation
- Cryptography and Data Security
- Data Quality and Management
- Protein Tyrosine Phosphatases
- Ion channel regulation and function
- Blood properties and coagulation
- Hemoglobinopathies and Related Disorders
- Access Control and Trust
- Air Quality and Health Impacts
- Data Mining Algorithms and Applications
- Simulation Techniques and Applications
- Neuroscience and Neuropharmacology Research
- Renin-Angiotensin System Studies
- Neuroscience of respiration and sleep
- Membrane-based Ion Separation Techniques
- Distributed systems and fault tolerance
- Cloud Data Security Solutions
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Erythropoietin and Anemia Treatment
- Data Management and Algorithms
- Semantic Web and Ontologies
Case Western Reserve University
2020-2025
University School
2020-2025
Institut National des Sciences Appliquées Centre Val de Loire
2022-2024
Université d'Orléans
2024
Centre Val de Loire
2022-2024
Laboratoire d'Informatique Fondamentale d'Orléans
2022-2024
Firoozgar General Hospital
2013
Iran University of Medical Sciences
2013
In this second of three papers, we examine the morphometry red blood cells (RBCs) from our laboratory mouse strain (C57BL/6Case) and RBC-membrane proteomics. paper #1, using stopped-flow absorbance-spectroscopy to ascertain rate constant for oxyhemoglobin (HbO 2 ) deoxygenation (k HbO2 ), find substantial reductions in k with (1) membrane-protein inhibitors p-chloromercuribenzenesulfonate (pCMBS) or 4,4'-diisothiocyanatostilbene-2,2'-disulfonate (DIDS); (2) knockouts aquaporin-1 (AQP1-KO),...
This Hypothesis & Theory contribution accompanies the research paper by Bouyer et al. (Frontiers in Physiology 2024), first to employ out-of-equilibrium (OOE) CO2/HCO3 - solutions examine systematically intracellular pH (pHi) effects of extracellular (o) metabolic acidosis (MAc) and its components: an isolated decrease pHo (pure acidosis, pAc) [HCO3 -]o metabolic/down, pMet↓). In this study, after reviewing various types acid-base disturbances use OOE solutions, we discuss pHi "state" (ΔpHi,...
Receptor protein tyrosine phosphatases γ and ζ (RPTPγ RPTPζ) are transmembrane signaling proteins with extracellular carbonic anhydrase–like domains that play vital roles in the development functioning of central nervous system (CNS) implicated tumor suppression, neurodegeneration, sensing [CO 2 ] [HCO 3 − ]. RPTPγ expresses throughout body, whereas RPTPζ preferentially CNS. Here, we investigate differential RPTPγ-RPTPζ expression three sources derived from a wild-type laboratory strain...
Abstract Many have believed that oxygen (O 2 ) crosses red blood cell (RBC) membranes by dissolving in lipids offer no resistance to diffusion. However, using stopped-flow (SF) analyses of hemoglobin (Hb) absorbance spectra during O off-loading from mouse RBCs, we now report most traverses membrane-protein channels. Two agents excluded the RBC interior markedly slow off-loading: p-chloromercuribenzenesulfonate (pCMBS) reduces inferred membrane permeability ( P Membrane ∼82%, and...
Due to the rapid proliferation of data online, an important quantity private or sensitive informations is being stored as linked in graph databases (e.g., represented RDF). For such be shared without jeopardizing privacy, they must first undergo a process known database sanitization. During this process, are transformed following transformations that usually described informally through ad-hoc processes. However, more thourough formalization these would aid analysing sanitization ensuring...
Receptor protein tyrosine phosphatase ζ (RPTPζ) is a transmembrane almost exclusively expressed in the CNS regulates cells adhesion and signaling during development. RPTPζ ‘s close homolog RPTPγ, senses extracellular CO₂/HCO₃⁻ modulates acid-base transport response to basolateral Δ[CO₂] Δ[HCO₃⁻] kidney. Here, we investigate if similarly defends against disturbances brain. We hypothesize that metabolic (MAc) or respiratory (RAc) acidosis ↑RPTPζ activity regulate signal cascades defend...
The traditional view had been that all O 2 moves through red blood cell (RBC) membranes by dissolving in and diffusing the membrane lipid. In a previous abstract, we reported data obtained using stopped‐flow analysis of hemoglobin (Hb) absorbance spectra on RBCs from wild‐type (WT) vs. double‐knockout (dKO) mice genetically deficient both aquaporin‐1 (AQP1) rhesus (Rh) protein RhAG. We found rate constant Hb deoxygenation ( k HbO2 )—which includes intra/extracellular diffusion...
The diffusion of O2 across the red blood cell (RBC) membrane is critical for life. Although traditional view had been that all moves through RBC membranes by dissolving in and diffusing lipid, our recent work (bioRxiv. doi:10.1101/2020.08.28.265066) indicates this not case. Using stopped-flow absorbance spectroscopy to monitor hemoglobin (Hb) spectrum intact RBCs, we found that, compared with RBCs from wild-type (WT) mice, those double knockout (dKO) mice genetically deficient both...
Receptor protein tyrosine phosphatases γ and ζ (RPTPγ & RPTPζ) are transmembrane signaling proteins that, as monomers, catalyze dephosphorylation of residues on cytosolic targets. RPTPγ RPTPζ unusual in having an extracellular carbonic-anhydrase–like domain (CALD). Carbonic anhydrases (CAs) the reaction, CO₂ + H₂O ⇌ HCO3– H⁺, but CALDs enzymatically inactive. Previous studies renal proximal tubules (PTs) strongly suggest that is a dual CO₂/HCO₃⁻ sensor. RPTPγ—expressed PT basal (i.e.,...