A5053343778- Cancer, Hypoxia, and Metabolism
- Cancer-related molecular mechanisms research
- Cancer, Lipids, and Metabolism
- TGF-β signaling in diseases
- Bone Tissue Engineering Materials
- Metabolism, Diabetes, and Cancer
- Lipid metabolism and disorders
- biodegradable polymer synthesis and properties
- Electrospun Nanofibers in Biomedical Applications
- Prostate Cancer Treatment and Research
- Collagen: Extraction and Characterization
- interferon and immune responses
- Ubiquitin and proteasome pathways
- 3D Printing in Biomedical Research
- RNA modifications and cancer
- Mathematical Biology Tumor Growth
- Knee injuries and reconstruction techniques
- Metabolomics and Mass Spectrometry Studies
- Estrogen and related hormone effects
- DNA Repair Mechanisms
- Graphene and Nanomaterials Applications
- Protein Degradation and Inhibitors
- Cellular Automata and Applications
- Facial Trauma and Fracture Management
- Spectroscopy Techniques in Biomedical and Chemical Research
Forschungsinstitut für Leder und Kunststoffbahnen
2014-2024
TU Dresden
2010-2013
Bayer (Germany)
2013
Jena University Hospital
2011
Friedrich Schiller University Jena
2008
Institute of Human Genetics
2007
Virtually all solid tumors are dependent on a vascular network to provide them with the right amount of nutrients and oxygen. In that sense, low oxygen tension or hypoxia leads an adaptive response is transcriptionally regulated by hypoxia-inducible factors (HIF), which tightly controlled HIF prolyl hydroxylases (PHD). this study, we show inhibition sensor PHD2 in tumor cells stimulates vessel formation but paradoxically results profound reduction growth. This effect relies antiproliferative...
For the regeneration or creation of functional tissues, biodegradable biomaterials including polylactic acid (PLA) are widely preferred. Modifications material surface quite common to improve cell-material interactions and thereby support biological outcome. Typical approaches include a wet chemical treatment with mostly hazardous substances functionalization plasma. In present study, gas-phase fluorination was applied functionalize PLA surfaces in simple one-step process. The response...
Androgens play a central role in prostate development and cancer proliferation. Induction of telomerase is an early event carcinogenesis considered as marker for both primary tumors metastases. Interestingly, several reports suggest that activity regulated by androgens vivo. Here, we show the wild-type (WT) human androgen receptor (AR) inhibits expression reverse transcriptase (hTERT) via inhibition hTERT promoter presence agonists. However, pure antagonists failed to repress transcription....
Extracts from Pygeum africanum are used in the treatment of prostatitis, benign prostatic hyperplasia and prostate cancer (Pca), major health problems men Western countries. The ligand-activated human androgen receptor (AR) supports growth gland. Inhibition AR by ablation therapy applying synthetic anti-androgens is therefore primary goal patients. Here, we show that atraric acid (AA) isolated bark material has anti-androgenic activity, inhibiting transactivation mediated AR. This...
The incorporation of bioactive ions into biomaterials has gained significant attention as a strategy to enhance bone tissue regeneration on the molecular level. However, little knowledge exists about effects addition these immune response and especially most important cellular regulators, macrophages. Thus, this study aimed investigate in vitro cytocompatibility vivo regulation remodeling material-related responses biphasic substitute (BBS) coated with metal (Sr 2+ /Mg ) PLGA, using pure BBS...
Reconstruction of ruptured anterior cruciate ligaments (ACLs) is limited by the availability and donor site morbidity autografts. Hence, a tissue engineered graft could present an alternative in future. This study was undertaken to determine performance lapine (L) ACL-derived fibroblasts on embroidered poly(l-lactide-co-ε-caprolactone) (P(LA-CL)) polylactic acid (PLA) scaffolds regard engineering approach for ACL reconstruction. Surface modifications P(LA-CL)/PLA gas-phase fluorination...
Solid tumor growth is intimately associated with angiogenesis, a process that efficiently triggered by hypoxia. Therefore, oxygen-sensitive signaling pathways are thought to play critical role in angiogenesis and progression. Here, the function of prolyl hydroxylase-4 (PHD4), relative hydroxylase domain proteins 1-3 promote degradation hypoxia-inducible factors (HIF), was interrogated. To test hypothesis PHD4 might inhibit it overexpressed osteosarcoma cells, unexpectedly, this manipulation...
Abstract Collagen is increasingly used as material for medical devices because of its excellent biocompatibility. To achieve long‐term durability, the collagen materials are dried and subsequently sterilized. Most common sterilization methods unsuitable they cause denaturation. Currently, only ethylenoxide or γ irradiation preserves microscopic macroscopic structure. However, both require high safety standards include risk uncontrolled crosslinking cleavage protein chains. Here,...
The repression of the androgen receptor (AR) activity is a major objective to inhibit prostate cancer growth. One underlying mechanism for efficient hormone therapy based on corepressors that inactivate AR. In line with this, castration-resistant associated malfunction or reduced corepressor action. To overcome overexpression endogenous corepressors, however, affects many other transcription factors. Therefore, an AR-specific could be advantage. Using yeast peptide aptamer two-hybrid screen...
Nutrient and oxygen transport, as well the removal of metabolic waste are essential processes to support maintain viable tissue. Current bioreactor technology used grow tissue cultures in vitro has a fundamental limit thickness tissues. Based on low diffusion maximum 200 μm is possible. The efficiency those systems currently under investigation. During cultivation process artificial bioreactors, which lasts 28 days or longer, there no possibilities investigate viability cells. This work...
Supplementary Figures 1-5, Tables 1-2 from Inhibition of HIF Prolyl Hydroxylase-2 Blocks Tumor Growth in Mice through the Antiproliferative Activity TGFβ
Supplementary Figures 1-5, Tables 1-2 from Inhibition of HIF Prolyl Hydroxylase-2 Blocks Tumor Growth in Mice through the Antiproliferative Activity TGFβ
<p>PDF file - 108K, S3. VEGF levels are not changed in PHD4-overexpressing LM8 tumors.</p>
<p>PDF file - 82K</p>
<p>PDF file - 133K, S5. Lentivirus-mediated silencing of HIF-2alpha in LM8 control cells.</p>
<p>PDF file - 40K, S2. Overexpression of PHD4 in LM8 osteosarcomas has no effect on the relative endothelial area.</p>
<p>PDF file - 101K, S4. Lentivirus-mediated silencing of TGF-alpha in LM8-PHD4 cells.</p>
<p>PDF file - 72K, S1. PHD4 overexpression in eEPCs reduces HIF-2alpha levels under hypoxia.</p>
<div>Abstract<p>Solid tumor growth is intimately associated with angiogenesis, a process that efficiently triggered by hypoxia. Therefore, oxygen-sensitive signaling pathways are thought to play critical role in angiogenesis and progression. Here, the function of prolyl hydroxylase-4 (PHD4), relative hydroxylase domain proteins 1–3 promote degradation hypoxia-inducible factors (HIF), was interrogated. To test hypothesis PHD4 might inhibit it overexpressed osteosarcoma cells,...
<div>Abstract<p>Solid tumor growth is intimately associated with angiogenesis, a process that efficiently triggered by hypoxia. Therefore, oxygen-sensitive signaling pathways are thought to play critical role in angiogenesis and progression. Here, the function of prolyl hydroxylase-4 (PHD4), relative hydroxylase domain proteins 1–3 promote degradation hypoxia-inducible factors (HIF), was interrogated. To test hypothesis PHD4 might inhibit it overexpressed osteosarcoma cells,...
<p>PDF file - 101K, S4. Lentivirus-mediated silencing of TGF-alpha in LM8-PHD4 cells.</p>
<p>PDF file - 82K</p>