A5058287768- Microtubule and mitosis dynamics
- RNA and protein synthesis mechanisms
- Genetic and Kidney Cyst Diseases
- Histone Deacetylase Inhibitors Research
- RNA Research and Splicing
- RNA modifications and cancer
- Cellular transport and secretion
- Ubiquitin and proteasome pathways
- Photoreceptor and optogenetics research
- Cellular Mechanics and Interactions
- Protist diversity and phylogeny
- Machine Learning in Bioinformatics
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Protein Degradation and Inhibitors
- Micro and Nano Robotics
Utrecht University
2024-2025
University of Wisconsin–Madison
2015-2024
Abstract Cilia are motile or sensory organelles present on many eukaryotic cells. Their formation and function rely axonemal microtubules, which exhibit very slow dynamics, but the underlying mechanisms largely unexplored. Here we reconstituted in vitro individual collective activities of ciliary tip module proteins CEP104, CSPP1, TOGARAM1, ARMC9 CCDC66, interact with each other microtubules and, when mutated humans, cause ciliopathies such as Joubert syndrome. We show that a protein...
Microtubules are essential to neuron shape and function. Acetylation of tubulin has the potential directly tune behavior function microtubules in cells. Although proteomic studies have identified several acetylation sites α-tubulin, effects at these remains largely unknown. This includes highly conserved residue lysine 394 (K394), which is located αβ-tubulin dimer interface. Using a fly model, we show that α-tubulin K394 acetylated nervous system an residue. We found acetylation-blocking...
Microtubules are essential for neuronal structure and function. Axonal dendritic microtubules enriched in post-translational modifications that impact microtubule dynamics, transport microtubule-associated proteins. Acetylation of α-tubulin lysine 40 (K40) is a prominent conserved modification microtubules. However, the cellular role acetylation remains controversial. To resolve how might affect morphogenesis, we mutated endogenous vivo using new Drosophila strain facilitates rapid knock-in...
A single protein can bind and regulate many mRNAs. Multiple proteins with similar specificities often control overlapping sets of Yet little is known about the architecture or dynamics overlapped networks. We focused on three structures related RNA-binding specificities-Puf3p, Puf4p, Puf5p S. cerevisiae Using RNA Tagging, we identified a "super-network" comprised four subnetworks: Puf3p, subnetworks, one controlled by both Puf4p Puf5p. The individual thus super-network, determined...
Abstract Cilia are essential motile or sensory organelles found on many eukaryotic cells. Their formation and function rely axonemal microtubules, which exhibit very slow dynamics, however the underlying biochemical mechanisms largely unexplored. Here, we reconstituted in vitro individual collective activities of ciliary tip module proteins, CEP104, CSPP1, TOGARAM1, ARMC9 CCDC66, interact with each other and, when mutated, cause ciliopathies such as Joubert syndrome. a protein containing...
During animal development, the spatiotemporal properties of molecular events largely determine biological outcomes. Conventional gene analysis methods lack resolution for precise dissection developmental mechanisms. Although optogenetic tools exist manipulating designer proteins in cultured cells, few have been successfully applied to endogenous live animals. Here, we report OptoTrap, a light-inducible clustering system diverse sizes, subcellular locations, and functions
ABSTRACT Animal development involves numerous molecular events, whose spatiotemporal properties largely determine the biological outcomes. Conventional methods for studying gene function lack necessary resolution precise dissection of developmental mechanisms. Optogenetic approaches are powerful alternatives, but most existing tools rely on exogenous designer proteins that produce narrow outputs and cannot be applied to diverse or endogenous proteins. To address this limitation, we developed...
ABSTRACT A single protein can bind and regulate many mRNAs. Multiple proteins with similar specificities often control overlapping sets of Yet, little is known about the architecture or dynamics overlapped networks. We focused on three structures related RNA-binding – Puf3p, Puf4p, Puf5p S. cerevisiae . Using RNA Tagging, we identified a “super-network” comprised four sub-networks: sub-networks, one controlled by both Puf4p Puf5p. The individual subnetworks, thus super-network, are...
ABSTRACT Microtubules are essential to neuronal structure and function. Axonal dendritic microtubules enriched in post-translational modifications that impact microtubule dynamics, transport, microtubule-associated proteins. Acetylation of α-tubulin lysine 40 (K40) is a prominent, conserved modification microtubules. However, the cellular role acetylation remains controversial. To resolve how might affect morphogenesis we mutated endogenous vivo using new fly strain facilitates rapid...