A5007311511- Plant Virus Research Studies
- RNA and protein synthesis mechanisms
- Plant and Fungal Interactions Research
- RNA modifications and cancer
- Bacteriophages and microbial interactions
- DNA and Nucleic Acid Chemistry
- Genomics and Phylogenetic Studies
- Plant Disease Resistance and Genetics
- RNA Research and Splicing
- Animal Virus Infections Studies
- Advanced biosensing and bioanalysis techniques
- Viral Infections and Immunology Research
- Geotechnical Engineering and Analysis
- CRISPR and Genetic Engineering
- Viral gastroenteritis research and epidemiology
- Tunneling and Rock Mechanics
- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
- Plant tissue culture and regeneration
- Protein Structure and Dynamics
- Geotechnical Engineering and Underground Structures
- RNA regulation and disease
- Plant Reproductive Biology
- Soil and Unsaturated Flow
- Advanced Proteomics Techniques and Applications
Heinrich Heine University Düsseldorf
2014-2024
Friedrich-Alexander-Universität Erlangen-Nürnberg
2022
Park Plaza Hospital
2022
Hochschule Düsseldorf University of Applied Sciences
2012
Graz University of Technology
2006-2011
Geotechnics (United Kingdom)
2005-2010
Federal Ministry of Finance
2009
University of Würzburg
2002
Rational (Germany)
1985
Darmstadt University of Applied Sciences
1980
Abstract Temperature‐gradient gel electrophoresis (TGGE) is applied to analyze conformational transitions and sequence variations of nucleic acids protein‐nucleic acid interactions. A linear highly reproducible temperature‐gradient established perpendicular or parallel the direction electrophoresis. The instrument consists an electrically insulated metal plate, which heated at one edge cooled other by two thermostating baths used as ancillary device for commercial horizontal instruments....
A program is described which calculates the thermal stability and denaturation behaviour of doublestranded DNAs RNAs up to a length 1000 base pairs. The algorithm based on recursive generation conditional priori probabilities for stacking. Output may be compared directly experimental results; thus used optimize nucleic acid fragments, primers conditions prior experiments like polymerase chain reactions, temperature-gradient gel electrophoresis, denaturinggradient electrophoresis...
The helix-coil transitions of the 16 octadecameric DNA duplexes dCGTCGTTTXACAACGTCG-dCGACGTTGTX'AAACGACG with A, T, G, and C for X 1 were measured by UV-absorption. This sequence was taken from former studies ui vivo determination efficiencies mismatch repair (Kramer, Kramer, Fritz (1984) Cell 38, 879–887). thermodynamic parameters double strand formation have been obtained evaluating partition function a stack model which allowed loop formation. As result mismatches could be classified into...
The performance of alignment programs is traditionally tested on sets protein sequences, which a reference known. Conclusions drawn from such benchmarks do not necessarily hold for the RNA problem, as was demonstrated in first benchmark published so far. For example, twilight zone - similarity range where quality drops drastically starts at 60 % RNAs comparison to 20 proteins. In this study we enhance previous benchmark.The sequence database are taken an increased number families avoid...
Abstract The hammerhead ribozyme (HHRz) is an autocatalytic RNA motif found in subviral plant pathogens and transcripts of repetitive DNA sequences animals. Here, we report the discovery characterization unique HHRzs encoded a genome. Two novel were identified on chromosome IV Arabidopsis thaliana database search, which took into account recently defined structural requirements. are expressed several tissues coexist vivo as both cleaved noncleaved species. In vitro, cleave efficiently at...
Strong viroid-caused pathogenesis was achieved in tomato cv. Rutgers by biolistic transfer of severe or lethal potato spindle tuber viroid (PSTVd) strains, while other genotypes (e.g., Moneymaker) were tolerant. With reciprocal hybrids between sensitive and tolerant genotypes, we show that plant depression dominates over tolerance. Biolistic the most pathogenic PSTVd strain AS1 to Nicotiana benthamiana, which is considered be a symptomless host, led strong reaction stunting, suggesting...
To defend against invading pathogens, plants possess RNA silencing mechanisms involving small RNAs (miRNAs, siRNAs). Also viroids - plant infectious, non-coding, unencapsidated cause the production of viroid-specific (vsRNA), but do escape cytoplasmic mechanism. Viroids with minor sequence variations can produce different symptoms in infected plants, suggesting an involvement vsRNAs symptom production. We analyzed by deep sequencing spectrum induced PSTVd strain AS1, which causes strong such...
Most lncRNAs display species-specific expression patterns suggesting that animal models of cancer may only incompletely recapitulate the regulatory crosstalk between and oncogenic pathways in humans. Among these pathways, Sonic Hedgehog (SHH) signaling is aberrantly activated several human entities. We unravel aberrant primate-specific lncRNA HedgeHog Interacting Protein-AntiSense 1 (HHIP-AS1) a hallmark SHH-driven tumors including medulloblastoma atypical teratoid/rhabdoid tumors. HHIP-AS1...
A model of functional elements critical for replication and infectivity the potato spindle tuber viroid (PSTVd) was proposed earlier: a thermodynamically metastable structure containing specific hairpin (HP II) in (−)-strand intermediate is essential template activity during (+)-strand synthesis. We present here detailed kinetic analysis on how PSTVd (−)-strands fold synthesis by sequential folding into variety structures that rearrange only slowly distribution thermodynamic equilibrium....
Abstract Motivation: Alignment of RNA has a wide range applications, for example in phylogeny inference, consensus structure prediction and homology searches. Yet aligning structural or non-coding RNAs (ncRNAs) correctly is notoriously difficult as these sequences may evolve by compensatory mutations, which maintain base pairing but destroy sequence homology. Ideally, alignment programs would take into account. The Sankoff algorithm the simultaneous solution was proposed 20 years ago suffers...
The two satellite RNAs associated with CYMV infections were sequenced. larger (sCYMV-L1) has only linear molecules 1145 nucleotides long, a poly(A) tail, long open reading frame (ORF) coding for protein of M r 39636 resembling in composition those other large nepovirus RNAs, 5′ leader sequence 16 and 3′ non-coding region 40 nucleotides. In vitro translation sCYMV-L1 yielded product size that corresponded to predicted from the sequence. smaller (sCYMV-S1) is 457 no ORF significant length...