A5031763870- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Wildlife Ecology and Conservation
- Reproductive Physiology in Livestock
- Animal Genetics and Reproduction
- Animal Behavior and Welfare Studies
- Ichthyology and Marine Biology
- Genetic and phenotypic traits in livestock
- Fish Ecology and Management Studies
- Animal Behavior and Reproduction
- Reproductive biology and impacts on aquatic species
- Veterinary Medicine and Surgery
- Marine and fisheries research
- Genetic diversity and population structure
- Primate Behavior and Ecology
- Seed Germination and Physiology
- Turtle Biology and Conservation
- Veterinary Equine Medical Research
- Genetic Mapping and Diversity in Plants and Animals
- Sexual Differentiation and Disorders
- Ovarian function and disorders
- Fish biology, ecology, and behavior
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Marine animal studies overview
- Neuroendocrine regulation and behavior
Cornell University
2021
Royal Oak Community Coalition
2021
California Animal Hospital
2021
Zoo Miami
2021
New York State College of Veterinary Medicine
2021
Smithsonian Conservation Biology Institute
2001-2013
National Zoological Park
2001-2003
Smithsonian Institution
1998-2001
Leibniz Institute for Zoo and Wildlife Research
1998
United States Department of Agriculture
1997-1998
The rediscovery of remnant Florida panthers (Puma concolor coryi) in southern swamplands prompted a program to protect and stabilize the population. In 1995, conservation managers translocated eight female pumas (P. c. stanleyana) from Texas increase depleted genetic diversity, improve population numbers, reverse indications inbreeding depression. We have assessed demographic, population-genetic, biomedical consequences this restoration experiment show that panther numbers increased...
Zoos and other ex situ wildlife institutions can play an important role in species conservation by maintaining populations for education research, as sources potential re-introduction or reinforcement, ambassadors financial support of conservation. However, many regional zoo associations are realizing that current captive unsustainable, with programs failing to meet demographic genetic goals ensure long-term viability. Constraints on population size due limited space often mandate delayed...
A new method for the cryopreservation of mouse spermatozoa was developed using a modified egg-yolk TES–Tris diluent containing 0.1% sodium lauryl sulfate and 1.25% (v/v) glycerol (mouse sperm cryoprotectant, MSC). Epididymal collected from 10-week-old CBA males were frozen at rate 5°C min−1 to 4°C 50°C −70°C programmable cell freezer. percentage (25%) regained motility after thawing. In vitro fertilization with frozen–thawed resulted in 50% oocytes developing two-cell stage. These embryos...
Hormone analysis is a precise and widely accepted tool for monitoring reproductive function responses to stressors. Although hormones are present can be measured in various biological matrices, non-invasive methods have gained popularity over the past 30 years as more practical approach assessing ovarian, testicular and, recently, adrenocortical activity intractable wildlife species. Non-invasive hormone also has been key understanding mechanisms related observed behaviours of captive...
The origins of giraffe's imposing stature and associated cardiovascular adaptations are unknown. Okapi, which lacks these unique features, is closest relative provides a useful comparison, to identify genetic variation underlying long neck system. genomes giraffe okapi were sequenced, through comparative analyses genes pathways identified that exhibit changes likely contribute features. Some in the HOX, NOTCH FGF signalling pathways, regulate both skeletal development, suggesting evolved...
About 1500 preantral follicles can be recovered from a single cat ovary by mechanical dissection. This is potentially rich source of genetic material if ova could preserved and grown in vitro, especially rare or endangered species that die abruptly are ovariectomized for medical reasons. The aims this study were to examine cryoprotectant toxicity then the potential successfully cryopreserving follicles. In initial trial, isolated (40–90 μm) exposed dimethylsulfoxide, glycerol, 1,2-propandiol...
Understanding the fundamental reproductive biology of a species is first step toward identifying parameters that are critical for reproduction and development assisted techniques. Ejaculates were collected from aquarium (n = 24) in situ 34) sand tiger sharks Carcharias taurus. Volume, pH, osmolarity, sperm concentration, motility, status, morphology, plasma membrane integrity assessed each ejaculate. Semen with highest proportion motile was between April June both indicating seasonal cycle....
A combination of flow cytometric sperm sorting X and Y chromosome-bearing (X sperm) computer-assisted analysis (CASA) for measuring motility allows assessment motion parameters in the two populations. Bull were separated into populations by cytometry following staining with DNA-binding dye Hoechst 33,342. The differed depending on concentration. Decreasing concentration resulted higher velocities straighter trajectories. concentrations control (stained-unsorted unstained-unsorted)...
Abstract An antiserum, designated R4 and raised against denatured hamster acrosomes, was shown to localize specifically the acrosomal region of hamster, rat, mouse, human spermatozoa, inhibit both sperm–oocyte binding in vitro. Following screening a testis λgt11 cDNA expression library with antiserum R4, series clones were isolated. One (cDNA 134) selected based on ability β‐galactosidase fusion protein sperm–zona The also penetration zona‐free oocytes by spermatozoa. Sequence analysis...
Abstract Non-lethal methods for semen collection from elasmobranchs to better understand species reproduction has accompanied the development of artificial insemination. Ejaculates (n = 82) collected whitespotted bamboo sharks Chiloscyllium plagiosum 19) were assessed and cold-stored raw or extended at 4 °C. Females 20) inseminated with fresh 24–48 h paternity offspring determined microsatellite markers. Insemination females 10) resulted in 80 hatchlings 27.6% fertility. 24 4) 48 1) 17...
This study characterized seasonal changes in circulating LH and testosterone semen production quality the Northern pintail duck. Plasma were measured blood samples collected weekly throughout year from eight males exposed to natural fluctuations day length temperature. Semen was evaluated these same April–June, months when spermatozoa produced. (based on sperm concentration normal morphology) peaked 0–2 weeks after onset decreased sharply before cessation late June. Nadir concentrations July...
Teratospermia (>60% of morphologically abnormal spermatozoa) is well documented in felids. Even normal spermatozoa from teratospermic ejaculates have reduced ability to undergo tyrosine phosphorylation, acrosome react, and bind penetrate oocytes compared with normospermic (<40% counterparts. However, it unknown whether fertilization deficiencies originate at a nuclear level. This study examined failure also was attributable sperm chromatin, using the chromatin structure assay (SCSA), vitro...
To characterize physical examination, plasma biochemical, and ultrasonographic findings in aquarium-housed, managed semiwild, wild southern stingrays (Hypanus americanus) with without reproductive disease.Southern from aquarium (n = 48), lagoon (managed semiwild; 34), (12) habitats.Limited, opportunistic prosections were performed of presumed anatomically normal compared for aquarium-housed disease. Ultrasonographic video data both groups used to assign a score (1 5) indicating increasing...
Northern pintail duck semen and sperm traits were characterized, the fertility of cold-stored spermatozoa was investigated using artificial insemination. Excellent quality ejaculates containing high proportions motile collected from drakes within 20 s by a massage technique. Semen in Beltsville poultry extender, pooled (4 degrees C) for 0, 24, 48 or 72 h. Hens inseminated with 100 microl twice week, eggs assessed fertilization hatch success. Fertilization success similar (P > 0.05) 0...
Abstract Although critical to the conservation of white rhinoceros, captive breeding has proven challenging because poor and irregular reproductive health many rhinos, social interactions may play a significant role. This research investigated spatial relationships two groups southern rhinoceros ( Ceratotherium simum ) by examining frequency companion changes, number space maintenance vocalizations made per hour each reproductively mature female, dominant/subordinate interactions. The...
Abstract Most ungulate species are herd animals. In captivity, and increasingly so in the wild, space constraints limit natural behaviors associated with group dynamics, possibly resulting inbreeding and/or overpopulation. This situation has necessitated research regarding contraception of various hoofstock. Differing management situations mandate different protocols to achieve optimal results. Fertility control hoofstock been achieved through a number contraceptive methods predominantly...