A5076589761- HIV Research and Treatment
- Virus-based gene therapy research
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Herpesvirus Infections and Treatments
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Probiotics and Fermented Foods
- Viral gastroenteritis research and epidemiology
- Animal Disease Management and Epidemiology
- Animal Virus Infections Studies
- Antimicrobial Peptides and Activities
- Gut microbiota and health
- Dermatology and Skin Diseases
- Veterinary Oncology Research
- Virology and Viral Diseases
- Hematopoietic Stem Cell Transplantation
- Rabies epidemiology and control
- Asthma and respiratory diseases
- Allergic Rhinitis and Sensitization
- Transgenic Plants and Applications
- Bacteriophages and microbial interactions
- Vector-Borne Animal Diseases
- Food Allergy and Anaphylaxis Research
- Monoclonal and Polyclonal Antibodies Research
Colorado State University
1996-2024
North Carolina State University
2005-2019
Memphis Zoo
2009
Chembio (United States)
2009
Howard Hughes Medical Institute
2009
National Animal Disease Center
2009
Albert Einstein College of Medicine
2009
Agricultural Research Service
2009
North Central State College
2005
ARUP Laboratories (United States)
2000
Feline immunodeficiency virus (FIV) is similar to human type 1 virologically and induces a clinical syndrome in cats comparable humans. To determine the lymphoid target cells of FIV, populations CD4+ lymphocytes, CD8+ CD21+ lymphocytes (B cells) were enriched more than 96.5% purity then analyzed for FIV provirus by semiquantitative DNA amplification. We found CD4+, CD8+, B lymphocytes. In infected <4 months, proviral burden was greatest cells, followed cells. 5 years, The total > log10...
ABSTRACT Oral vaccines that elicit a mucosal immune response may be effective against human immunodeficiency virus type 1 (HIV-1) because its transmission occurs mainly at the mucosa. The aim of this study was to construct recombinant Lactobacillus for oral delivery HIV-1 and evaluate their immunogenicity. A acidophilus strain expressing Gag on bacterial cell surface established by fusion with signal peptide anchor motif mucus binding protein (Mub) from L. or without coexpression Salmonella...
ABSTRACT Lactobacillus species are commensal bacteria that have long been recognized as probiotic microbes and generally regarded safe (GRAS) for human consumption. We investigated the use of L. gasseri a vaccine vector oral immunization against mucosal pathogens. Recent research has shown immune response to different lactobacilli can vary widely depending on or subspecies being studied. While some seem induce tolerance, others an adaptive response. This study characterized systemic...
Abstract: The objectives of the present study were to characterize and compare repertoire cytokine‐genes transcribed in skin homogenates obtained from normal dogs with atopic dermatitis (AD) using a reverse‐transcriptase polymerase chain reaction canine‐specific cytokine‐gene primers. Whereas IL‐4 IL‐5 transcripts detected more commonly biopsy homogenates, IL‐2 mRNA was amplified often control specimens. IFN‐γ 5/29 specimens, 4 them only dog chronic lesions. One‐fourth samples exhibited...
ABSTRACT Display of heterologous antigens on the cell surface is considered a useful technique for vaccine delivery by recombinant lactobacilli. In this study, two Lactobacillus acidophilus derivatives displaying Salmonella flagellin (FliC) were constructed using different anchor motifs. one instance, FliC protein was fused to C-terminal region envelope proteinase (PrtP) and bound wall electrostatic bonds. other case, same antigen conjugated mucus binding (Mub) covalently associated with an...
The FeLV-FAIDS strain of feline leukemia virus consistently induces fatal immunodeficiency. To investigate the immunopathogenesis and viral genetic determinants responsible for induction immunodeficiency disease in vivo, we have generated chimeras between two major genomes original isolate, designated common form clone 61E variant 61C, which were molecularly cloned directly from DNA same animal tissue. Each three 61E/C chimeras, containing at minimum a 34-amino-acid segment (including...
Surface layer proteins of probiotic lactobacilli are theoretically efficient epitope-displaying scaffolds for oral vaccine delivery due to their high expression levels and surface localization. In this study, we constructed genetically modified Lactobacillus acidophilus strains expressing the membrane proximal external region (MPER) from human immunodeficiency virus type 1 (HIV-1) within context major S-layer protein, SlpA. Intragastric immunization mice with recombinants induced...
The feline thymus is a target organ and site of viral replication during the acute stage immunodeficiency virus (FIV) infection. This was demonstrated by histologic, immunohistologic, flow cytometric, virologic tests. Thymic lesions developed after 28 days postinoculation (p.i.) included thymitis, premature cortical involution, medullary B-cell hyperplasia with germinal center formation epithelial distortion. Alterations in thymocyte subsets also developed. Fewer CD4+ CD8- cells were...
Cytauxzoonosis is an emerging infectious disease of domestic cats (Felis catus) caused by the apicomplexan protozoan parasite Cytauxzoon felis. The growing epidemic, with its high morbidity and mortality points to need for a protective vaccine against cytauxzoonosis. Unfortunately, causative agent has yet be cultured continuously in vitro, rendering traditional development approaches beyond reach. Here we report use comparative genomics computationally experimentally interpret C. felis...
ABSTRACT Independent studies have demonstrated different cell tropisms for molecular clones of feline immunodeficiency virus (FIV). In this report, we examined three clones, FIV-pF34, FIV-14, and FIV-pPPR, replication in Crandell kidney (CrFK) cells, peripheral blood mononuclear cells (PBMC), macrophage cultures. Importantly, tropism these was also vivo. FIV-pF34 efficient CrFK but severely restricted PBMC, whereas FIV-pPPR vigorous PBMC cells. FIV-14 productive both PBMC. Interestingly, all...
Type 1 and 2 cytokine mRNA responses were measured at various time periods in lymphoid compartments during the acute stage (first 4 months) of feline immunodeficiency virus (FIV) infection laboratory cats. Cytokine correlated with replication. Virus was detected plasma tissue from day 14 postinfection (p.i.) onward, peaked 56 to 70 days, declined greatly by days. replication highest thymus, followed spleen, mesenteric lymph nodes, cervical nodes. Baseline levels nodes lowest upregulation...
Lesional skin of cats with allergic dermatitis has a cellular infiltrate and CD4/CD8 ratio comparable to that in humans atopic dermatitis. CD4+ helper T cells particular belonging the Th2 subset play an important role disease pathogenesis humans. We investigated cytokine pattern situ, special emphasis on putative presence producing interleukin 4 (IL4), Immunohistochemical procedures were used determine lesional nonlesional can produce IL4, as occurs had significantly more IL4+ (P = 0.001)...
ABSTRACT Recombinant Listeria monocytogenes has many attractive characteristics as a vaccine vector against human immunodeficiency virus (HIV). Wild-type and attenuated strains expressing HIV Gag have been shown to induce long-lived mucosal systemic T-cell responses in mice. Using the feline (FIV) model of we evaluated recombinant L. challenge system. Five cats were immunized with that expresses FIV delivers an Env-expressing DNA (LM gag /pND14-Lc- env ). Control either sham or wild-type...