A5026401395- Plant Virus Research Studies
- Microbial Community Ecology and Physiology
- Wheat and Barley Genetics and Pathology
- Gut microbiota and health
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Insect Pheromone Research and Control
- Plant Disease Resistance and Genetics
- Plant Pathogens and Fungal Diseases
- Mycotoxins in Agriculture and Food
- Plant tissue culture and regeneration
- Insect and Arachnid Ecology and Behavior
- Chromosomal and Genetic Variations
- Plant and Fungal Interactions Research
- Insect and Pesticide Research
- Inflammatory Bowel Disease
- Metabolomics and Mass Spectrometry Studies
- Agronomic Practices and Intercropping Systems
- Forest Insect Ecology and Management
- Environmental DNA in Biodiversity Studies
- Plant and animal studies
- Viral gastroenteritis research and epidemiology
- Protist diversity and phylogeny
- Vibrio bacteria research studies
- Molecular Biology Techniques and Applications
Dalhousie University
2014-2024
Université Laval
1993-2019
Palo Alto Veterans Institute for Research
2018
University of California, Irvine
2018
Fukuoka University
2018
Forêt Méditerranéenne
2014
Agriculture and Agri-Food Canada
2001-2013
Université Toulouse III - Paul Sabatier
2007-2011
Laboratoire de Microbiologie et Génétique Moléculaires
2005-2011
Université de Toulouse
2009-2010
Sequence-based approaches to study microbiomes, such as 16S rRNA gene sequencing and metagenomics, are uncovering associations between microbial taxa a myriad of factors. A drawback these is that the necessary library preparation bioinformatic analyses complicated continuously changing, which can be barrier for researchers new field. We present three essential components conducting microbiome experiment from start finish: first, simplified step-by-step custom protocol requires limited lab...
Identifying differentially abundant microbes is a common goal of microbiome studies. Multiple methods are used interchangeably for this purpose in the literature. Yet, there few large-scale studies systematically exploring appropriateness using these tools interchangeably, and scale significance differences between them. Here, we compare performance 14 differential abundance testing on 38 16S rRNA gene datasets with two sample groups. We test amplicon sequence variants operational taxonomic...
Increasing global temperatures are having a profound impact in the Arctic, including dramatic loss of multiyear sea ice 2007 that has continued to present. The majority life Arctic is microbial and consequences climate-mediated changes on marine food webs, which responsible for biogeochemical cycling support higher trophic levels, unknown. We examined communities over time by using high-throughput sequencing DNA collected between 2003 2010 from subsurface chlorophyll maximum (SCM) layer...
Although the multiplication of bacteriophages (phages) has a substantial impact on biosphere, comparatively little is known about how external environment affects phage production. Here we report that sub-lethal concentrations certain antibiotics can substantially stimulate host bacterial cell's production some virulent phage. For example, low dosage cefotaxime, cephalosporin, increased an uropathogenic Escherichia coli strain's PhiMFP by more than 7-fold. We name this phenomenon...
High-depth sequencing of universal marker genes such as the 16S rRNA gene is a common strategy to profile microbial communities. Traditionally, sequence reads are clustered into operational taxonomic units (OTUs) at defined identity threshold avoid errors generating spurious units. However, there have been numerous bioinformatic packages recently released that attempt correct determine real biological sequences single nucleotide resolution by amplicon variants (ASVs). As more researchers...
trans-8, trans-10-Dodecadien-1-ol is a sex attractant of the codling moth, Laspeyresia pomonella. Antennal responses (electroantennograms) to series monounsaturated compounds were used in determining location and configuration two double bonds. The synthetic compound very attractive male moths field.
In aquatic environments, fungal communities remain little studied despite their taxonomic and functional diversity. To extend the ecological coverage of this group, we conducted an in-depth analysis sequences within our collection 3.6 million V4 18S rRNA pyrosequences originating from 319 individual marine (including sea-ice) freshwater samples libraries generated diverse projects studying Arctic temperate biomes in past decade. Among ~1.7 post-filtered reads highest phylogenetic quality,...
Crohn's disease (CD) has an unclear etiology, but there is growing evidence of a direct link with dysbiotic microbiome. Many gut microbes have previously been associated CD, these mainly confounded patients' ongoing treatments. Additionally, most analyses CD microbiomes focused on in stool samples, which yield different insights than profiling biopsy samples. We sequenced the 16S rRNA gene (16S) and carried out shotgun metagenomics (MGS) from intestinal biopsies 20 treatment-naïve control...
Meromictic lakes are useful biogeochemical models because of their stratified chemical gradients and separation redox reactions down the water column. Perennially ice-covered meromictic particularly stable, with long term constancy in density profiles. Here we sampled Lake A, a deep lake at latitude 83°N High Arctic Canada. Sampling was before (May) after (August) an unusual ice-out event during warm 2008 summer. We determined bacterial archaeal community composition by high-throughput 16S...
In metagenomic analyses of microbiomes, one the first steps is usually taxonomic classification reads by comparison to a database previously taxonomically classified genomes. While different studies comparing methods have determined that tools are 'best', there two been used most to-date: Kraken (
Sex pheromone specificity is the only obvious reproduction isolating mechanism for two tortricid species and gelechiid species. Pheromones of are cis-9-tetradecenyl acetate trans-9-tetradecenyl acetate. Male response one inhibited by other This could be important sympatric evolutionary saltation.
The tail fiber adhesins are the primary determinants of host range in T4-type bacteriophages. Among indispensable virion components, sequences long genes and their associated among most variable. predominant form adhesin phages is not even version gene encoded by T4, archetype superfamily, but rather a small unrelated protein (gp38) closely related such as T2 T6. This gp38 has modular design: its N-terminal attachment domain binds at tip fiber, whereas C-terminal specificity determines...
The fundamental role of bacteria in global biogeochemical cycles warrants a thorough understanding the factors controlling bacterial community structure. In this study, integrated effect seasonal differences and spatial distribution on structure diversity were investigated at regional scale. We conducted comprehensive survey, with 451 samples Scotian Shelf sector Northwest Atlantic Ocean during spring fall 2014 2016, to analyze effects physicochemical gradients Throughout region,...
The Escherichia coli bacteriophage T4 has served as a classic system in phage biology for more than 60 years. Only recently have phylogenetic analyses and genomic comparisons demonstrated the existence of large, diverse, widespread superfamily T4-like phages environment. We report here on major capsid protein (MCP) sequences that were obtained by targeted polymerase chain reaction (PCR) marine environmental samples. This analysis was then expanded to include 1,000s new genes from metagenomic...
Abstract The sex pheromone of the female eastern spruce budworm was identified as trans -11-tetradecenal by a combination electroantennogram techniques, laboratory bioassays, and chemical analysis. Subsequent field trapping showed that this compound is potent attractant for both male Choristoneura fumiferana (Clem.) C . occidentalis Free, thus supporting its identification suggesting it also major component in system