A5033986617- Fecal contamination and water quality
- Parasitic Infections and Diagnostics
- Legionella and Acanthamoeba research
- Viral gastroenteritis research and epidemiology
- Vibrio bacteria research studies
- Water Treatment and Disinfection
- Child Nutrition and Water Access
- Amoebic Infections and Treatments
- Wastewater Treatment and Reuse
- Bacteriophages and microbial interactions
- Pediatric health and respiratory diseases
- Cerebrospinal fluid and hydrocephalus
- Virus-based gene therapy research
- Salmonella and Campylobacter epidemiology
- Viral Infections and Immunology Research
- Pharmaceutical and Antibiotic Environmental Impacts
- Metallurgical Processes and Thermodynamics
- Nuclear reactor physics and engineering
- Travel-related health issues
- Animal Virus Infections Studies
- Aquaculture disease management and microbiota
- Hepatitis Viruses Studies and Epidemiology
- Environmental Justice and Health Disparities
- Molecular Biology Techniques and Applications
- Biosensors and Analytical Detection
Centers for Disease Control and Prevention
2014-2024
Waterborne Environmental (United States)
2015-2024
University of Cincinnati
2022-2023
Cincinnati Children's Hospital Medical Center
2023
Neighborhood House
2023
National Center for Emerging and Zoonotic Infectious Diseases
2012-2021
Center for Surveillance, Epidemiology, and Laboratory Services
2018-2021
AID Atlanta
2021
Epidemic Intelligence Service
2020
University of North Carolina at Chapel Hill
1998-2019
Provision of safe water in the United States is vital to protecting public health (1). Public agencies U.S. states and territories* report information on waterborne disease outbreaks CDC through National Outbreak Reporting System (NORS) (https://www.cdc.gov/healthywater/surveillance/index.html). During 2013-2014, 42 drinking water-associated† were reported, accounting for at least 1,006 cases illness, 124 hospitalizations, 13 deaths. Legionella was associated with 57% these all Sixty-nine...
ABSTRACT Noroviruses (NoV), which are members of the family Caliciviridae , most important cause outbreaks acute gastroenteritis worldwide and commonly found in shellfish grown polluted waters. In present study, we developed broadly reactive one-step TaqMan reverse transcription (RT)-PCR assays for detection genogroup I (GI) GII NoV fecal samples, as well samples. The specificity sensitivity all steps were systematically evaluated, final format, monoplex validated by using RNA extracted from...
Abstract Provision of safe drinking water in the United States is a great public health achievement. However, new waterborne disease challenges have emerged (e.g., aging infrastructure, chlorine-tolerant and biofilm-related pathogens, increased recreational use). Comprehensive estimates burden for all exposure routes (ingestion, contact, inhalation) sources (drinking, recreational, environmental) are needed. We estimated total illnesses, emergency department (ED) visits, hospitalizations,...
Advances in water management and sanitation have substantially reduced waterborne disease the United States, although outbreaks continue to occur. Public health agencies U.S. states territories* report information on CDC Waterborne Disease Outbreak Surveillance System (http://www.cdc.gov/healthywater/surveillance/index.html). For 2011-2012, 32 drinking water-associated were reported, accounting for at least 431 cases of illness, 102 hospitalizations, 14 deaths. Legionella was responsible 66%...
Wastewater surveillance, the measurement of pathogen levels in wastewater, is used to evaluate community-level infection trends, augment traditional surveillance that leverages clinical tests and services (e.g., case reporting), monitor public health interventions (1). Approximately 40% persons infected with SARS-CoV-2, virus causes COVID-19, shed RNA their stool (2); therefore, trends SARS-CoV-2 infections, both symptomatic asymptomatic (2) can be tracked through wastewater testing (3-6)....
ABSTRACT A quantitative real-time TaqMan PCR assay for detection of human adenoviruses (HAdV) was developed using broadly reactive consensus primers and a probe targeting conserved region the hexon gene. The correctly identified 56 representative adenovirus prototype strains field isolates from all six species (A to F). Based on infectious units, able detect as few 0.4 0.004 units serotype 2 (AdV2) AdV41, respectively, with results obtained in less than 90 min. Using genomic equivalents, 5...
ABSTRACT The ability to simultaneously concentrate diverse microbes is an important consideration for sample collection methods that are used emergency response and environmental monitoring when drinking water may be contaminated with array of unknown microbes. This study focused on developing a concentration method using ultrafilters different combinations chemical dispersant (sodium polyphosphate [NaPP]) surfactants. Tap samples were seeded bacteriophage MS2, Escherichia coli ,...
Ultrafiltration (UF) is increasingly being recognized as a potentially effective procedure for concentrating and recovering microbes from large volumes of water treated wastewater. Because their very small pore sizes, UF membranes are capable simultaneously viruses, bacteria, parasites based on size exclusion. In this study, UF-based sampling was used to recover representatives these three microbial classes seeded into 100-liter samples tap collected eight cities covering six hydrologic...
Background. Naegleria fowleri is a climate-sensitive, thermophilic ameba found in the environment, including warm, freshwater lakes and rivers. Primary amebic meningoencephalitis (PAM), which almost universally fatal, occurs when N. fowleri–containing water enters nose, typically during swimming, migrates to brain via olfactory nerve. In 2011, 2 adults died Louisiana hospitals of infectious after brief illnesses.
Outbreaks of infection with hepatitis E virus (HEV) are frequently attributed to contaminated drinking water, even if direct evidence for this is lacking.We conducted several epidemiologic investigations during a large HEV outbreak in Uganda.Of 10,535 residents, 3218 had infection; these, 2531 lived households >1 case. was not detected water or zoonotic sources. Twenty-five percent cases occurred > = 8 weeks after onset an index case the household. Households 2 were more likely have...
ABSTRACT Dead-end ultrafiltration (DEUF) is an alternative approach to tangential-flow hollow-fiber that can be readily employed under field conditions recover microbes from water. The hydraulics of DEUF and microbe recovery for a new method were investigated using 100-liter tap water samples. Pressure, flow rate, temperature four ultrafilter types. Based on hydraulic performance, the Asahi Kasei REXEED 25S was selected experiments. Microbe experiments performed MS2 bacteriophage,...
The implementation of treated municipal water systems in the 20th century led to a dramatic decrease waterborne disease United States. However, communities with deficient still experience outbreaks. In August 2004, we investigated an outbreak gastroenteritis on South Bass Island, Ohio, island 900 residents that is visited by >500,000 persons each year.To identify source illness, conducted case-control study and environmental investigation. A case was defined as diarrhea person who traveled...
Inactivation of infectious viruses during drinking water treatment is usually achieved with free chlorine. Many utilities in the United States now use monochloramine as a secondary disinfectant to minimize by-product formation and biofilm growth. The inactivation human adenoviruses 2, 40, 41 (HAdV2, HAdV40, HAdV41), coxsackieviruses B3 B5 (CVB3 CVB5), echoviruses 1 11 (E1 E11), murine norovirus (MNV) are compared this study. Experiments were performed 0.2 mg chlorine or monochloramine/liter...
There is a critical need for developing new malaria diagnostic tools that are sensitive, cost effective and capable of performing large scale diagnosis. The real-time PCR methods particularly robust screening they can be used in control elimination programs. We have designed novel self-quenching photo-induced electron transfer (PET) fluorogenic primers the detection P. falciparum Plasmodium genus by PCR. A total 119 samples consisting different species mixed infections were to test utility...
Untreated recreational water is that has not undergone a disinfection or treatment process to maintain good microbiological quality for recreation.recreational pathogens, toxins, chemicals aerosolized volatilized from into the surrounding air.Public health officials in 50 states, District of Columbia, U.S. territories, and Freely Associated States † can voluntarily report water-associated outbreaks CDC.This focuses on data two groups untreated outbreaks: 1) those began during 2000-2012 were...
Abstract Background Produce-associated outbreaks of Shiga toxin–producing Escherichia coli (STEC) were first identified in 1991. In April 2018, New Jersey and Pennsylvania officials reported a cluster STEC O157 infections associated with multiple locations restaurant chain. The Centers for Disease Control Prevention (CDC) queried PulseNet, the national laboratory network foodborne disease surveillance, additional cases began investigation. Methods A case was defined as an infection between...