A5100412746- Parasitic Infections and Diagnostics
- Amoebic Infections and Treatments
- Viral gastroenteritis research and epidemiology
- Coccidia and coccidiosis research
- Helminth infection and control
- Toxoplasma gondii Research Studies
- Dermatological diseases and infestations
- Parasites and Host Interactions
- Clostridium difficile and Clostridium perfringens research
- Parasite Biology and Host Interactions
- Congenital Anomalies and Fetal Surgery
- Malaria Research and Control
- Study of Mite Species
- Legionella and Acanthamoeba research
- HIV Research and Treatment
- Parasitic infections in humans and animals
- Enterobacteriaceae and Cronobacter Research
- Pneumocystis jirovecii pneumonia detection and treatment
- Fecal contamination and water quality
- Pediatric health and respiratory diseases
- Veterinary medicine and infectious diseases
- Herpesvirus Infections and Treatments
- Microbial infections and disease research
- Cytomegalovirus and herpesvirus research
- Child Nutrition and Water Access
South China Agricultural University
2010-2025
Yancheng Third People's Hospital
2023-2025
Nantong University
2023-2025
Nanjing Medical University
2025
Jiangsu Vocational College of Medicine
2025
Zhejiang University of Technology
2025
Key Laboratory of Guangdong Province
2020-2024
Second Xiangya Hospital of Central South University
2023-2024
Central South University
2023-2024
Second Affiliated Hospital of Fujian Medical University
2022-2024
The current outbreak of coronavirus disease-2019 (COVID-19) poses unprecedented challenges to global health1. new responsible for this outbreak—severe acute respiratory syndrome 2 (SARS-CoV-2)—shares high sequence identity SARS-CoV and a bat coronavirus, RaTG132. Although bats may be the reservoir host variety coronaviruses3,4, it remains unknown whether SARS-CoV-2 has additional species. Here we show that which name pangolin-CoV, isolated from Malayan pangolin 100%, 98.6%, 97.8% 90.7% amino...
ABSTRACT Biological data support the hypothesis that there are multiple species in genus Cryptosporidium , but a recent analysis of available genetic suggested is insufficient evidence for differentiation. In order to resolve controversy taxonomy this parasite genus, we characterized small-subunit rRNA genes parvum baileyi muris and serpentis performed phylogenetic . Our study revealed contains phylogenetically distinct C. which consistent with biological characteristics host specificity...
To address the source of infection in humans and public health importance Giardia duodenalis parasites from animals, nucleotide sequences triosephosphate isomerase (TPI) gene were generated for 37 human isolates, 15 dog 8 muskrat 7 isolates each cattle beavers, 1 isolate a rat rabbit. Distinct genotypes found humans, cattle, dogs, muskrats, rats. TPI small subunit ribosomal RNA (SSU rRNA) G. microti muskrats also analyzed. Phylogenetic analysis on confirmed formation distinct groups....
To assess the genetic diversity in Cryptosporidium parvum, we have sequenced small subunit (SSU) rRNA gene of seven spp., various isolates C. parvum from eight hosts, and a isolate desert monitor. Phylogenetic analysis SSU sequences confirmed multispecies nature genus Cryptosporidium, with at least four distinct species (C. baileyi, muris, serpentis). Other previously defined by biologic characteristics, including wrairi, meleagridis, felis, monitor isolate, clustered together or within...
Cryptosporidium parvum is usually considered to be the pathogen responsible for human cryptosporidiosis. We genotyped in 132 stool specimens from 80 Peruvian children, representing 85 infection episodes, using techniques that differentiate species and C. genotypes. Five types of were identified: (67), bovine (8), dog (2) genotypes, meleagridis (7), felis (1). Twenty-five (29%) episodes associated with diarrhea. There was no significant difference age, antecedent stunting, percentage...
ABSTRACT Cryptosporidium parvum and hominis isolates from human immunodeficiency virus-infected patients, cattle, wild ruminants were characterized by PCR DNA sequencing analysis of the 60-kDa glycoprotein gene. Seven alleles identified, three corresponding to C. four . One new allele was found (IId), one (IIb) had only been in Portugal. Isolates cattle clustered two alleles. In contrast, seven alleles, showing extensive allelic diversity.
To understand the transmission of Cryptosporidium infection in children, fecal specimens from 62 Kuwaiti children with gastrointestinal symptoms found to be positive by microscopy were genotyped and subtyped a small subunit rRNA-based PCR-restriction fragment length polymorphism analysis 60-kDa glycoprotein-based DNA sequencing tool. The median age infected was 4.5 years, 77% infections occurred during cool season November April. Fifty-eight (94%) had parvum, three (5%) hominis, one (1%)...
ABSTRACT. The structure and infectivity of the oocysts a new species Cryptosporidium from feces humans are described. Oocysts structurally indistinguishable those parvum . passed fully sporulated, lack sporocysts, measure 4.4–5.4 μm (mean = 4.86) × 4.4–5.9 5.2μm) with length to width ratio 1.0–1.09 1.07) (n 100). were not infectious for ARC Swiss mice, nude Wistar rat pups, puppies, kittens or calves, but neonatal gnotobiotic pigs. Pathogenicity studies in pig model revealed significant...
Abstract To determine whether clinical manifestations are associated with genotypes or subtypes of Cryptosporidium spp., we studied a 4-year longitudinal birth cohort 533 children in Peru. A total 156 infection episodes were found 109 children. Data from first infections showed that C. hominis was diarrhea, nausea, vomiting, general malaise, and increased oocyst shedding intensity duration. In contrast, parvum, meleagridis, canis, felis diarrhea only. subtype families identified (Ia, Ib, Id,...
Molecular diagnostic tools have been used increasingly in the characterization of transmission cryptosporidiosis and microsporidiosis developing countries. However, few studies examined distribution Cryptosporidium species Enterocytozoon bieneusi genotypes AIDS patients receiving antiretroviral therapy. In present study, 683 HIV-positive National Free Antiretroviral Therapy Program China matched HIV-negative controls were enrolled. subtypes detected differentiated by PCR DNA sequencing. The...
A review was conducted to examine published works that focus on the complex epidemiology of Enterocytozoon bieneusi infection in humans. Studies prevalence these emerging microsporidian pathogens humans, developed and developing countries, different clinical spectra E. intestinal children, settings, risk factors associated with have been reviewed. This paper also analyses impact recent application PCR-based molecular methods for species-specific identification genotype differentiation has...
Abstract The outbreak of 2019-nCoV in the central Chinese city Wuhan at end 2019 poses unprecedent public health challenges to both China and rest world 1 . new coronavirus shares high sequence identity SARS-CoV a newly identified bat 2 While bats may be reservoir host for various coronaviruses, whether has other hosts is still ambiguous. In this study, one isolated from Malayan pangolins showed 100%, 98.2%, 96.7% 90.4% amino acid with E, M, N S genes, respectively. particular,...