A5001640638- SARS-CoV-2 and COVID-19 Research
- DNA Repair Mechanisms
- Microtubule and mitosis dynamics
- Genomics and Chromatin Dynamics
- Viral gastroenteritis research and epidemiology
- Fungal and yeast genetics research
- Viral Infections and Outbreaks Research
- RNA and protein synthesis mechanisms
- interferon and immune responses
- Microbial Metabolism and Applications
- Computational Drug Discovery Methods
- Microbial Inactivation Methods
- Magnetic and Electromagnetic Effects
- Bacteriophages and microbial interactions
- Cellular Mechanics and Interactions
- Transgenic Plants and Applications
- CRISPR and Genetic Engineering
- Viral Infections and Immunology Research
- RNA modifications and cancer
- Bacterial Genetics and Biotechnology
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- melanin and skin pigmentation
- Skin and Cellular Biology Research
- Microfluidic and Bio-sensing Technologies
The Francis Crick Institute
2020-2021
Chromosome 18 Registry & Research Society
2020
Cancer Research UK
2004-2009
University of Cambridge
1997-2000
MRC Laboratory for Molecular Cell Biology
1998
University College London
1998
The Honourable Society of Lincoln's Inn
1997
University of California, Berkeley
1997
National Institute for Medical Research
1984-1985
The Cdc6 protein is essential for the assembly of pre-replicative complexes (pre-RCs) at origins DNA replication in budding yeast Saccharomyces cerevisiae. This reaction blocked vivo by cyclin-dependent kinase Cdc28p, together with its regulatory subunits, B type cyclins that are present throughout S, G2, and M phases. Because destruction consequent inactivation exit from mitosis, pre-RC formation can only occur after passage through mitosis. Therefore, has been proposed to be coupling S...
The coronavirus disease 2019 (COVID-19) pandemic, which is caused by severe acute respiratory syndrome 2 (SARS-CoV-2), a global public health challenge. While the efficacy of vaccines against emerging and future virus variants remains unclear, there need for therapeutics. Repurposing existing drugs represents promising potentially rapid opportunity to find novel antivirals SARS-CoV-2. encodes at least nine enzymatic activities that are potential drug targets. Here, we have expressed,...
SARS-CoV-2 is responsible for COVID-19, a human disease that has caused over 2 million deaths, stretched health systems to near-breaking point and endangered economies of countries families around the world. Antiviral treatments combat COVID-19 are currently lacking. Remdesivir, only antiviral drug approved treatment can affect severity, but better needed. encodes 16 non-structural proteins (nsp) possess different enzymatic activities with important roles in viral genome replication,...
The COVID-19 pandemic has emerged as the biggest life-threatening disease of this century. Whilst vaccination should provide a long-term solution, is pitted against constant threat mutations in virus rendering current vaccines less effective. Consequently, small molecule antiviral agents would be extremely useful to complement program. causative agent novel coronavirus, SARS-CoV-2, which encodes at least nine enzymatic activities that all have drug targeting potential. papain-like protease...
The COVID-19 pandemic has presented itself as one of the most critical public health challenges century, with SARS-CoV-2 being third member Coronaviridae family to cause a fatal disease in humans. There is currently only antiviral compound, remdesivir, that can be used for treatment COVID-19. To identify additional potential therapeutics, we investigated enzymatic proteins encoded genome. In this study, focussed on viral RNA cap methyltransferases, which play key roles enabling protein...
AbstractThe Dbf4p/Cdc7p protein kinase is essential for the activation of replication origins during S phase. The catalytic subunit, Cdc7p, present at constant levels throughout cell cycle. In contrast, we show here that regulatory Dbf4p, oscillate Dbf4p absent from cells G1and accumulates and G2 phases. rapidly degraded time chromosome segregation remains highly unstable pre-Start G1 rapid degradation requires a functional anaphase-promoting complex (APC). Mutation sequence in N terminus...
Mutation of the d y e gene Escherichia coli results in sensitivity to dyes, envelope protein changes, loss expression alkaline phosphatase, and reduced transcription sex factor F genes.We have determined DNA sequence a 1.4-kilobase pair fragment encompassing gene.The coding was identified as an open reading frame for M, 27,346.A 64 residues at amino terminus extremely acidic, with 12 aspartic plus glutamic acid only 2 lysine arginine residues.A 19 adjacent near center identical, except one...
SARS-CoV-2 is a coronavirus that emerged in 2019 and rapidly spread across the world causing deadly pandemic with tremendous social economic costs. Healthcare systems worldwide are under great pressure, there an urgent need for effective antiviral treatments. The only currently approved treatment COVID-19 remdesivir, inhibitor of viral genome replication. proliferation relies on enzymatic activities non-structural proteins (nsp), which makes them interesting targets development new With aim...
Journal Article New vectors for expression of the E.coli lacZ gene in Dictyostelium Get access A.J. Harwood, Harwood ICRF Clare Hall LaboratoriesBlanche Lane, South Mimms, Potters Bar, Herts EN6 3LD, UK Search other works by this author on: Oxford Academic PubMed Google Scholar L. Drury Nucleic Acids Research, Volume 18, Issue 14, 25 July 1990, Page 4292, https://doi.org/10.1093/nar/18.14.4292 Published: 1990 history Received: 17 May
The coronavirus disease 2019 (COVID-19) global pandemic has turned into the largest public health and economic crisis in recent history impacting virtually all sectors of society. There is a need for effective therapeutics to battle ongoing pandemic. Repurposing existing drugs with known pharmacological safety profiles fast cost-effective approach identify novel treatments. COVID-19 etiologic agent severe acute respiratory syndrome 2 (SARS-CoV-2), single-stranded positive-sense RNA virus....
Deletions of the Escherichia coli K-12 chromosome between trpR and thr render bacterium sensitive to dye toluidine blue (Dye-), if male (Hfr or F'), strain is sterile (Fex-), failing donate F' chromosomal markers resistant male-specific phages as a consequence its inability elaborate F pili. A 6-kilobase SalI fragment E. DNA cloned into plasmid pBR322 has been shown complement both Dye- Fex- phenotypes. Insertion transposon gamma delta (Tn1000) specific part this invariably results in...
The use of an electrical field to permeabilize cells reversibly (electroporation) has become a valuable technique for transference DNA into both eukaryotic and prokaryotic cells. Many species bacteria have been successfully electroporated () many strains E. coli are routinely electrotransformed efficiencies 109 1010 transformants/µg DNA. Frequencies transformation can be as high 80% the surviving capacities nearly 10 µg transforming DNA/mL possible ().
Mutations of the dye gene on E. coli chromosome result in sensitivity to toluidine blue and, male cells, cause loss F pili, producing sterility conjugation. Compared with its dye+ parent, a strain deleted for (delta dye) showed an altered wide range dyes and antibiotics which affect different intracellular processes, hence it appeared likely that barrier properties cell envelope were impaired. Unlike mutants known be defective LPS structure, there no correlation between hydrophobicity...
Abstract Origins of eukaryotic DNA replication are ‘licensed’ during G1 phase the cell cycle by loading six related minichromosome maintenance (MCM) proteins into a double hexameric ring around double-stranded DNA. In S phase, some hexamers (MCM DHs) converted active CMG ( C dc45- M CM- G INS) helicases which nucleate assembly bidirectional forks. The remaining unfired MCM DHs act as ‘dormant’ origins to provide backup replisomes in event fork stalling. fate is unknown. Here we show that...
Summary Dominant mutations of the cet gene Escherichia coli result in tolerance to colicin E2 and increased amounts an inner membrane protein with M r 42 000. We have cloned + sequenced its DNA, revealing that product, coded by longest open‐reading frame, has 49772, five predicted transmembrane structures towards carboxy terminus one at amino terminus. demonstrated locus does fact code for is present mutants, we shown this amount Cet enhanced transcription. The be same operon as phoM gene,...
A mutant of Dictyostelium that is aberrant in the process tip formation (dtfA-: defective A) has been isolated by gene tagging. The dtfA predicted to encode a protein 163 kDa. There are no extensive sequence homologies between DTFA and previously identified proteins, but four short N-terminal motifs show partial homology repeats found mammalian mucins. Immunofluorescence reveals lattice-like arrangement at cell surface. When developing on bacterial lawn, cells strain (dtfA- cells) aggregate...
Origins of eukaryotic DNA replication are ‘licensed’ during G1 phase the cell cycle by loading six related minichromosome maintenance (MCM) proteins into a double hexameric ring around double-stranded DNA. In S phase, some hexamers (MCM DHs) converted active CMG (Cdc45-MCM-GINS) helicases which nucleate assembly bidirectional forks. The remaining unfired MCM DHs act as ‘dormant’ origins to provide backup replisomes in event fork stalling. fate is unknown. Here we show that cannot remove DHs....