The study of red blood cell (RBC) aggregation is great interest because its implications for human health. Altered RBC can lead to microcirculatory problems as in vascular pathologies, such hypertension and diabetes, due a decrease the erythrocyte surface electric charge an increase ligands present plasma. process was studied stasis situation (free shear stresses), using optical chip based on laser transmission technique. Kinetic curves under different conditions were obtained, allowing...

Traditional techniques to evaluate the aggregation of red blood cells by optical methods require large sample volume and provide parameters that vary significantly from one method another. A simplified variant a chip system previously developed Shin et al. (2009)¹ based on light transmission for measuring erythrocyte is presented. Through detailed analysis intensity versus time curves, relevant information about its variables obtained. Parameters more accuracy diagnosis patients...

Possible changes in the erythrocyte membrane, by vitro interaction with newborn larvae of T. spiralis (NL), were evaluated analyzing alterations aggregation digital image analysis and laser transmission a new optical chip aggregometer. NL obtained from CBi mice infected spiralis. RBCs samples healthy donors where exposed to (concentration (3000 ± 500) larvae/mL) assess its effect on RBC aggregation. Individual cell Coefficient (CCA) parameter (S) calculated digitally processing aggregate...

Hair cells of the auditory and vestibular systems are capable detecting sub-nanometer deflections due to air-borne or ground-borne vibrations. Further, hair bundles some end organs known spontaneously oscillate without external stimulation, indicating presence internal active processes. Ionic influx upon deflection elicits changes in somatic potential, leading a conversion mechanical input electrical signals. While transduction process has been well-studied cells, possible distortions cell...

Biophysical models describing complex cellular phenomena typically include systems of nonlinear differential equations with many free parameters. While experimental measurements can fix some parameters, those internal processes frequently remain inaccessible. Hence, a proliferation parameters risks overfitting the data, limiting model's predictive power. In this study, we develop systematic methods, applying statistical analysis and dynamical-systems theory, to reduce parameter count in...

In the last few decades, a large number of medicinal plants popularly used for treatment diabetes have been studied around world (Chang et al., 2013). The chemical characterization plant components and biological, pharmacological or toxicological activity different extracts well studied; however, more research is necessary on relationship between phytochemical their specific therapeutic action. Trigonelline an alkaloid present in leaves Bauhinia sp., known as “cow-hoof”, also coffee...

the bloodstream, until its encystment in muscle cell [3].A key to understanding parasitic infections is study of interactions established between two organisms involved: host and parasite.The existence more than 50 sialic acid structures recognized, which are located at end molecules

Biophysical models describing complex, cellular phenomena typically include systems of nonlinear differential equations with many free parameters. While experimental measurements can fix some parameters, those internal processes frequently remain inaccessible. Hence, a proliferation parameters risks overfitting the data, limiting model's predictive power. In this study, we develop robust methods, applying statistical analysis and dynamical-systems theory, to reduce biophysical complexity. We...

The study of erythrocyte (RBC) aggregation is great interest because its implications on human health, alterations in can lead to microcirculatory problems. An optical-chip based system was developed using laser transmission techniques order evaluate and characterize RBC aggregation. Studies are carried out with vitro altered by non-enzymatic glycosylation. Several samples were analyzed, computational data processing, characteristic parameters found, describing kinetics improve the early...

We investigated the erythrocyte interaction by means of biospeckle laser analysis. The Discrete Wavelet Transform was used to decompose sample activity in order better identify cells behavior. Correlation Coefficient calculated.

In this work, we studied the nature of molecular bonds involved in red blood cell aggregation process by coherent anti-Stokes Raman spectroscopy technique. Images were acquired with a commercial Leica TCS SP8 CARS confocal microscope (Leica Microsystems GmbH, Wetzlar, Germany) temporally and spatially overlapping pulses two sources focal plane microscope. A pump wavelength 810 nm to 817 was used for mode simultaneously Stokes beam at 1064 excite vibratory resonance symmetric hydrocarbon...

Hair cells of the auditory and vestibular systems are capable detecting sounds that induce sub-nanometer vibrations. These specialized convert mechanical deflections due to sound waves into electrical signals relayed brain through afferent neurons. This transduction involves mechanically gated ion channels open following deflection mechanoreceptive hair bundles reside on top these cells. Moreover, internal energy-consuming processes in bundle can amplify even generate innate oscillations...

We analyze the erythrocyte aggregation by biospeckle laser phenomenon and calculate characteristic parameters to develop non-invasive hemorheological techniques. Biospeckle is more complex than other light-based techniques, such as light transmission, providing information about dynamics of scatterers, their size, morphology. Dextran 500 kDa combined with plasma phosphate-buffered saline was used induce controlled in vitro aggregation. Red blood cell studied using an objective speckle setup...