A5002768538- Soil Carbon and Nitrogen Dynamics
- Soil and Unsaturated Flow
- Plant Water Relations and Carbon Dynamics
- Crop Yield and Soil Fertility
- Soil and Water Nutrient Dynamics
- CRISPR and Genetic Engineering
- Horticultural and Viticultural Research
- RNA and protein synthesis mechanisms
- Peatlands and Wetlands Ecology
- Reproductive System and Pregnancy
- Advanced biosensing and bioanalysis techniques
- Hydrology and Watershed Management Studies
- Mass Spectrometry Techniques and Applications
- Hungarian Social, Economic and Educational Studies
- Soil erosion and sediment transport
- Bioenergy crop production and management
- Analytical Chemistry and Chromatography
- Pasture and Agricultural Systems
- Pregnancy and preeclampsia studies
- RNA regulation and disease
- Agriculture Sustainability and Environmental Impact
- Neurogenesis and neuroplasticity mechanisms
- Glycosylation and Glycoproteins Research
- Galectins and Cancer Biology
- Soil Management and Crop Yield
HUN-REN Research Centre for Natural Sciences
2015-2024
Centre for Agricultural Research
2014-2024
Agrárközgazdasági Intézet
2021-2024
Institute of Molecular Life Sciences
2016-2023
University of Birmingham
2019-2022
Institute of Organic Chemistry
2012-2022
Hungarian Research Network
2022
Hungarian Academy of Sciences
2007-2020
Sapienza University of Rome
2020
Institute for Soil Sciences
2005-2019
The propensity for off-target activity of Streptococcus pyogenes Cas9 (SpCas9) has been considerably decreased by rationally engineered variants with increased fidelity (eSpCas9; SpCas9-HF1). However, a subset targets still generate considerable effects. To deal specifically these targets, we generated new "Highly enhanced Fidelity" nuclease (HeFSpCas9s) containing mutations from both eSpCas9 and SpCas9-HF1 examined improved side to decipher the factors that affect their specificities...
Abstract The widespread use of Cas12a (formerly Cpf1) nucleases for genome engineering is limited by their requirement a rather long TTTV protospacer adjacent motif (PAM) sequence. Here we have aimed to loosen these PAM constraints and generated new mutant variants the four orthologs that are active in mammalian plant cells, combining mutations corresponding RR RVR with altered specificities. LbCas12a-RVRR showing highest activity was selected an in-depth characterization its preferences...
Climate change is expected to have a vigorous impact on soils and ecosystems due elevated temperature changes in precipitation (amount frequency), thereby altering biogeochemical hydrological cycles. Several phenomena associated with climate anthropogenic activity affect indirectly via ecosystem functioning (such as higher atmospheric CO 2 concentration N deposition). Continuous interactions between determine the transformation transport processes. Long-term gradual abiotic environmental...
Cpf1s, the RNA-guided nucleases of class II clustered regularly interspaced short palindromic repeats system require a motive called protospacer adjacent motif (PAM) to be present next targeted sequence for their activity. The TTTV PAM As- and LbCpf1 is relatively rare in genome higher eukaryotic organisms. Here, we show that two other Cpf1 nucleases, Fn- MbCpf1, which have been reported utilize shorter, more frequently occurring (TTN) when tested vitro, carry out efficient modification...
Galectins are potent immunomodulators that regulate maternal immune responses in pregnancy and prevent the rejection of semi-allogeneic fetus also occurs miscarriages. We previously identified a gene cluster on Chromosome 19 expresses subfamily galectins, including galectin-13 (Gal-13) galectin-14 (Gal-14), which emerged anthropoid primates. These galectins expressed only by placenta induce apoptosis activated T lymphocytes, possibly contributing to shifted balance pregnancy. The placental...
Abstract This paper sets out the future potential and challenges for developing an interdisciplinary, mixed-method Citizen Social Science approach to researching urban emotions. It focuses on stress, which is increasingly noted as a global mental health challenge facing both urbanised rapidly urbanising societies. The reviews existing use of mobile psychophysiological or biosensing within environments—as means ‘capturing’ geographies Methodological reflections are included primary research...
The objective of this study was to investigate the effect soil tillage and crop type on respiration (Rs) in a typical Central European agricultural site characterized by rotation. weekly Rs supporting environmental variables were measured under different types (including winter summer crops) for 5 consecutive years mouldboard ploughing (MP) no-tillage (NT) treatments. long-term mean 0.093 mg CO2 m−2 s−1 NT versus 0.086 MP. Soil significantly higher treatment regardless weather conditions...
Cpf1 nucleases have recently been repurposed for site-specific genome modification. Two members of the family, AsCpf1 from Acidaminococcus sp. and LbCpf1 Lachnospiraceae bacterium were shown to induce higher indel frequencies than SpCas9 when examining four randomly-selected target sequences each type nuclease. Whether they are a real match Cas9 nucleases, however, remains be verified. Here, we used homology directed repair, either single strand annealing (SSA) or homologous recombination...
Abstract Soil hydraulic properties are among the most importantparameters that determine soil quality and its capabilityto serve ecosystem. Land use can significantlyinfluence properties, including conditions;however, additional factors, such as changes in climate(temperature precipitation), further influencethe land effects on properties. Inorder to develop possible adaptation measures mitigateany negative of climatic changes,it is important study impact changesin In this paper,we summarize...
Abstract Increased fidelity mutants of the SpCas9 nuclease constitute most promising approach to mitigating its off-target effects. However, these variants are effective only in a restricted target space, and many them reported work less efficiently when applied clinically relevant, pre-assembled, ribonucleoprotein forms. The low tolerance 5′-extended, 21G-sgRNAs contributes, great extent, their decreased performance. Here, we report generation Blackjack variant that shows increased yet...
Termed as galectin-13, placental protein 13 (PP13) is exclusively expressed in the placenta of anthropoid primates. Research on PP13 normal and pathologic pregnancies show alteration concentrations pregnancy affected by preeclampsia or gestational diabetes. Galectins are also described potent immunomodulators, regulates T cell function placenta. Therefore, this study aims to investigate effects neutrophils; a type often ignored pregnancy, but present uterus from early stages pregnancy. Since...
Abstract. Terrestrial biogeochemical models are essential tools to quantify climate–carbon cycle feedback and plant–soil relations from local global scale. In this study, a theoretical basis is provided for the latest version of Biome-BGCMuSo model (version 6.2). branch original Biome-BGC with large number developments structural changes. Earlier versions performed poorly in terms soil water content (SWC) dynamics different environments. Moreover, lack detailed nitrogen representation was...
Abstract With continued advancements in portable eye-tracker technology liberating experimenters from the restraints of artificial laboratory designs, research can now collect gaze data real-world, natural navigation. However, field lacks a robust method for achieving this, as past approaches relied upon time-consuming manual annotation eye-tracking data, while previous attempts at automation lack necessary versatility in-the-wild navigation trials consisting complex and dynamic scenes....
Land use and management affect soil hydrological processes, the impacts can be further enhanced accelerated due to climate change. In this study, we analyzed possible long-term effects of different land types on processes based future climatic scenarios. Soil moisture temperature probes were installed at four sites, a cropland, vineyard, meadow, forest area. Based modeling changes in water content (SWC) using HYDRUS 1D model, found that precipitation have more pronounced effect than air...
Miscarriages affect 50–70% of all conceptions and 15–20% clinically recognized pregnancies. Recurrent pregnancy loss (RPL, ≥2 miscarriages) affects 1–5% Nevertheless, our knowledge about the etiologies pathophysiology RPL is incomplete, thus, reliable diagnostic/preventive tools are not yet available. Here, we aimed to define diagnostic value three placental proteins for RPL: human chorionic gonadotropin free beta-subunit (free-β-hCG), pregnancy-associated plasma protein-A (PAPP-A), growth...
This paper presents and compares two different strategies in the numerical simulation of passive microfluidic mixers based on chaotic advection. In addition to flow velocity field calculations, concentration distributions molecules trajectories microscale particles were determined compared evaluate performance applied modeling approaches proposed geometries. A staggered herringbone type micromixer (SHM) was selected studied order demonstrate finite element issues. The microstructures...
Understanding the roles of natural drivers and anthropogenic activities in greenhouse gas (GHG) emissions arable fields is crucial for adopting most appropriate agricultural management. This study investigated effect two tillage treatments mouldboard ploughing (MP) no-tillage (NT), environmental factors (soil water content temperature, carbon nitrogen forms) on soil dioxide (CO2) nitrous oxide (N2O) emissions. The research was conducted chernozem under winter wheat cultivation. Besides field...
A mathematical model was applied for the Bükk Mountains (Hungary) to evaluate effects of climate change on soil water balance elements and regime. Model runs using SWAP were performed combinations four distinctive types three land use systems arable land, grassland, forest. The temporal variation regime under changing climatic conditions examined considering no cover occurring in future. data consisted predictions two regional models, Swiss CLM Swedish RCA. RCA results showed 45% 50% 5% 14%...