A5080100384- Cancer therapeutics and mechanisms
- DNA Repair Mechanisms
- Synthesis and Biological Evaluation
- Drug Transport and Resistance Mechanisms
- RNA Interference and Gene Delivery
- Bioactive Compounds and Antitumor Agents
- Carcinogens and Genotoxicity Assessment
- Amino Acid Enzymes and Metabolism
- Cancer-related Molecular Pathways
- DNA and Nucleic Acid Chemistry
- Virus-based gene therapy research
- Cancer Research and Treatments
- Education and Critical Thinking Development
- Effects of Radiation Exposure
- Microbial Natural Products and Biosynthesis
- Advanced Fluorescence Microscopy Techniques
- Histone Deacetylase Inhibitors Research
- Service-Learning and Community Engagement
- Natural Compound Pharmacology Studies
- Higher Education Research Studies
- Acute Lymphoblastic Leukemia research
- 14-3-3 protein interactions
- Pediatric Hepatobiliary Diseases and Treatments
- Neuroendocrine regulation and behavior
- Radiation Therapy and Dosimetry
University of Calgary
2011-2024
University of Toronto
2024
O'Brien Institute
2019
Moffitt Cancer Center
2015-2016
University of Florida
2015-2016
National Cancer Institute
2016
Center for Cancer Research
2016
Newcastle University
2013
Queen's University
1992-2001
University of Colorado Health
2000-2001
The doxorubicin-selected lung cancer cell line H69AR is resistant to many chemotherapeutic agents. However, like most tumor samples from individuals with this disease, it does not overexpress P-glycoprotein, a transmembrane transport protein that dependent on adenosine triphosphate (ATP) and associated multidrug resistance. Complementary DNA (cDNA) clones corresponding messenger RNAs (mRNAs) overexpressed in cells were isolated. One cDNA hybridized an mRNA of 7.8 8.2 kilobases was 100-...
The requirement for the serine/threonine protein kinase ATM in coordinating cellular response to DNA damage induced by ionizing radiation has been studied extensively. Many of anti-tumor chemotherapeutics clinical use today cause double strand breaks; however, few have evaluated their ability modulate ATM-mediated pathways. We investigated doxorubicin, a topoisomerase II-stabilizing drug. Using several ATM-proficient and ATM-deficient cell lines, we observed ATM-dependent nuclear...
The metabolism and effects of (+)-S- (-)-R-abscisic acid (ABA) some metabolites were studied in maize (Zea mays L. cv Black Mexican Sweet) suspension-cultured cells. Time-course studies metabolite formation performed both cells medium via analytical high-performance liquid chromatography. Metabolites isolated identified using physical chemical methods. At 10 [mu]M concentration 28[deg] C, (+)-ABA was metabolized within 24 h, yielding natural (-)-phaseic [(-)-PA] as the major product....
Myxoma virus (MYXV) is a promising oncolytic agent and highly effective against immortalized glioma cells but less brain tumor initiating (BTICs), which are believed to mediate development/recurrence. MYXV encodes various proteins attenuate host cell apoptosis, including an antiapoptotic Bcl-2 homologue known as M011L. Such may limit the ability of kill BTICs, have heightened resistance apoptosis. We hypothesized that infecting BTICs with M011L-deficient construct would overcome BTIC MYXV.We...
Topoisomerase II (topo II) is a ubiquitous enzyme that essential for cell survival through its role in regulating DNA topology and chromatid separation. Topo can be poisoned by common chemotherapeutics (such as doxorubicin etoposide), leading to the accumulation of cytotoxic enzyme-linked double-stranded breaks. In contrast, nonbreak-inducing topo catalytic inhibitors have also been described more limited use clinical chemotherapy. These agents, however, may alter efficacy regimens...
Brain tumor-initiating cells (BTICs) are stem-like hypothesized to form a disease reservoir that mediates tumor recurrence in high-grade gliomas. Oncolytic virotherapy uses replication-competent viruses target and kill malignant has been evaluated clinic for glioma therapy with limited results. Myxoma virus (MyxV) is safe highly effective oncolytic (OV) conventional models but, as seen other OVs, only modestly patient-derived BTICs. The objective of this study was determine whether MyxV...
Human DNA topoisomerase IIalpha (topo II), a ubiquitous nuclear enzyme, is essential for normal and neoplastic cellular proliferation survival. Several common anticancer drugs exert their cytotoxic effects through interaction with topo II. In experimental systems, altered II expression has been associated the appearance of drug resistance. This mechanism, however, does not adequately account clinical cases resistance to II-directed drugs. Modulation by protein-protein interactions represents...
The ATP-binding cassette transporter multidrug resistance protein 1 (MRP1) confers to a number of clinically important chemotherapeutic agents. proximal promoter region <i>MRP1</i> is GC-rich and contains binding sites for members the Sp1 family <i>trans</i>-acting factors that seem be basal expression. As an approach searching other elements may contribute expression, we have sequenced functionally compared promoters murine rat <i>mrp1</i> genes with human gene. All three are GC-rich,...
Inquiry-based learning provides students with an opportunity to take ownership of their while developing important higher order skills necessary for designing innovative solutions complex modern health problems. In our undergraduate sciences program, critical thinking, creativity, research and thinking are core program outcomes, thus inquiry-based is pillar curriculum. We have taken a staged approach, integrating (IBL) into each year four-year degree that scaffolds structure independence...
Haloacetonitriles (HANs), a class of nitrogen-containing disinfection by-products found in treated drinking water, are cytotoxic and genotoxic to mammalian cells. However, most cell toxicity data has been ascertained using transformed or cancer-derived animal human lines with an ambiguous relevance health. In this study, we evaluated the cytotoxicity individual chloro-, bromo-, iodo-acetonitrile (ClCH2CN, BrCH2CN, ICH2CN) their mixtures normal tissue-derived epithelium-derived RPE-1hTERT The...