Extracellular matrix plays an important role in regulating cell growth and differentiation. The biomimetic approach of cell-based tissue engineering is based on mirroring this vivo micro environment for developing a functional engineered construct. In study, we treated normal culture plates with selected extracellular components consisting glycosaminoglycans such as chondroitin-4-sulphate, dermatan sulphate, chondroitin-6-sulphate, heparin hyaluronic acid. Mesenchymal stem cells isolated...

Gaps in our understanding of genetic susceptibility to malignant hyperthermia (MH) limit the application and interpretation diagnosis condition. Our aim was define prevalence role variants three genes implicated MH largest comprehensively phenotyped cohort worldwide.We initially included one individual from each positive family tested UK Unit since 1971 detect RYR1, CACNA1S, or STAC3. Screening for has been ongoing 1991 involved a range techniques, most recently next generation sequencing....

Nowadays, the industry uses 3D Scanners for reverse engineering, new product design, rapid manufacturing, multimedia, architecture, inspection, and quality control. The scanning process converts a real object into digital format. This paper's essential purpose is to show use of blue light Scanner/COMET redesign carburetor body. paper identifies different issues involved in processes help future users. COMET does body by which COLIN software used measurements, editing, analyzing acquired...

Abstract Background: Variants in RYR1 are associated with the majority of cases malignant hyperthermia (MH), a form heat illness pharmacogenetically triggered by general anesthetics, and they have also been exertional (EHI). CACNA1S has implicated MH. The authors applied targeted next-generation sequencing approach to identify variants cohort unrelated patients diagnosed MH susceptibility. They provide first comprehensive report these two genes survivors EHI. Methods: DNA extracted from...

Pfizer in the USA has pioneered an orally active drug called Nirmatrelvir (PF-07321332) targeting main protease (Mpro) protein of SARS-CoV-2. However, current scenario demands additional scaffolds with greater potency and binding affinity. Various computational techniques were employed to detect new scaffold comparison Mpro. These included shape-based screening, molecular docking, dynamic simulation (MDS), ADMET studies. The query molecule for screening ZINC database was PF-07321332. In...

Abstract Oxy‐bromination of cyclic amines to corresponding α,α’‐dibromocycloamide(s) using bromide‐bromate couple in aqueous acetic acid and at 60 °C (oil bath) is presented the current work. A various types were successfully oxybrominated during this Separation desired product by filtration therefore avoiding need column chromatography, use as safe stable source oxy‐bromination agent, mild reaction conditions so on are merits present research method. In silico ADMET target prediction...

<title>Abstract</title> Background Encephalitis, an inflammatory disorder of the brain caused by infections or autoimmune responses, is still a major global health concern due to its high morbidity, mortality, and long-term neurological consequences. The available therapy alternatives are usually confined, conventional drug discovery procedures both resource-intensive time-consuming. These challenges underscore critical need for novel tools speed up development, which could revolutionize...

Abstract Introduction The absence of mutation or promoter hypermethylation in the BRCA2 gene majority breast cancer cases has indicated alternative ways its involvement, deregulated expression being one possibility. We show how a polymorphism 5' untranslated region (UTR) can serve as such factor. Based on hypothesis that variants genes involved same pathway influence risk provided for cancer, status p53 codon 72 was also investigated and possible interaction between polymorphisms examined....

The essential oils are fragrant products whose complex compositions obtained from various parts of plants by dry or steam distillation. Plants with variable biological activities have been explored worldwide. presence a large number phenols, terpenes and other aromatic compounds make more precise in their mode action. Because this, they known to possess many like antimicrobial, antioxidant anti-inflammatory etc. In this article, we will review the published literature summarizing chemistry...

DNA is one of the most important biomolecules living cells which carries genetic information from generation to generation. Many endogenous and exogenous agents may disrupt structure DNA. Change in cellular genome can lead errors replication, transcription protein synthesis. damage occurs naturally or result a metabolic hydrolytic process release some very active chemical entities like free radicals, Reactive Oxygen Species (ROS), Nitrogen Intermediate (RNI), Carbonyl (RCS), lipid...

Abstract Mitochondrial DNA alterations in recent years have been suggested as modifier events, providing a possible proliferative advantage to the tumor cells. In order provide further insight into process of tumorigenesis, study whole mitochondria genome was conducted 134 tissue samples obtained from 2 unrelated cancers (tumor and adjacent normal tissues 36 breast cancer 31 esophageal squamous cell carcinoma (ESCC) patients) with known p53 somatic mutation background. Fifteen (41.66%) 12...

Abstract Background- Sporadic breast cancer like many other cancers is proposed to be a manifestation of abnormal genetic and epigenetic changes. For the past decade our laboratory has identified genes involved in DNA damage response (DDR), apoptosis immunesurvelliance pathways influence sporadic risk north Indian population. Further enhance knowledge at level, we performed methylation study involving 17 gene promoter regions belonging (DDR) death receptor apoptotic pathway 162 paired normal...

Abstract: Nanomaterial-based therapeutics is an emerging tool for the treatment of numerous types cancer. Various polymeric, lipid and inorganic nanoparticles (NPs) result in a wider series applications cancer diagnosis therapeutics. The NPs properties are due to high surface area volume ratio, plasmon resonance, absorption visible spectrum light scattering. These unique characteristics arise their optical conjugation/surface modification smaller size. In therapeutics, based products used as...

Repurposing of drugs/natural or synthetic chemicals is a promising approach to identify the new therapeutic indication/use and mode action. In pharmaceuticals, this process used save time cost for drug discovery with reduced risk failure. present studies, repurposing natural molecule: sabinene (major phytochemical in cardamom) was characterize biological activities using silico as well vitro approaches. similarity searching demonstrated that (+)-3-carene possessed maximum structural...