A5020332354- Cellular Mechanics and Interactions
- Microtubule and mitosis dynamics
- Cardiomyopathy and Myosin Studies
- Muscle Physiology and Disorders
- Immunotherapy and Immune Responses
- Skin and Cellular Biology Research
- Cell Adhesion Molecules Research
- Blood properties and coagulation
- T-cell and B-cell Immunology
- Neurobiology and Insect Physiology Research
- Autophagy in Disease and Therapy
- melanin and skin pigmentation
- Reproductive System and Pregnancy
- Viral Infectious Diseases and Gene Expression in Insects
- Nuclear Structure and Function
- Reproductive Biology and Fertility
- Cancer Cells and Metastasis
- Silk-based biomaterials and applications
- Glycosylation and Glycoproteins Research
- Nerve injury and regeneration
- Fungal Infections and Studies
- Force Microscopy Techniques and Applications
- Cellular transport and secretion
- 3D Printing in Biomedical Research
- Muscle metabolism and nutrition
Rutgers, The State University of New Jersey
2009-2024
Rutgers Sexual and Reproductive Health and Rights
1991-2012
Nagoya University
1979-2000
Hiroshima University
1999
University of Arizona
1999
Vanderbilt University
1999
Rutgers Cancer Institute of New Jersey
1999
Cancer Institute of Florida
1999
Harvard University
1994
University of Guelph
1989
ROCK (Rho-kinase), an effector molecule of RhoA, phosphorylates the myosin binding subunit (MBS) phosphatase and inhibits activity. This inhibition increases phosphorylation light chain (MLC) II, which is suggested to induce RhoA-mediated assembly stress fibers focal adhesions. also known directly phosphorylate MLC in vitro; however, physiological significance this kinase activity unknown. It not clear whether alone sufficient for We have developed two reagents with opposing effects on...
We examined the role of regulatory myosin light chain (MLC) phosphorylation II in cell migration fibroblasts. Myosin kinase (MLCK) inhibition blocked MLC at periphery, but not center. MLCK-inhibited cells did assemble zyxin-containing adhesions maintained focal They generated membrane protrusions all around cell, turned more frequently, and migrated less effectively. In contrast, Rho-associated (ROCK) center, periphery. ROCK-inhibited assembled moved faster straight. On other hand,...
Fascin is an actin-bundling protein that found in membrane ruffles, microspikes, and stress fibers. The expression of fascin greatly increased many transformed cells, as well specialized normal cells including neuronal antigen-presenting dendritic cells. A morphological characteristic common to these expressing high levels the development protrusions which predominantly present. To examine whether contributes alterations microfilament organization at cell periphery, we have expressed LLC-PK1...
Phosphorylation of the regulatory light chain myosin II (RMLC) at Serine 19 by a specific enzyme, MLC kinase, is believed to control contractility actomyosin in smooth muscle and vertebrate nonmuscle cells. To examine how such phosphorylation regulated space time within cells during coordinated cell movements, including locomotion division, we generated phosphorylation-specific antibody. Motile fibroblasts with polarized shape exhibit bimodal distribution phosphorylated along direction...
Abstract Multiple isoforms of tropomyosin (TM) rat cultured cells show differential effects on actin-severing activity gelsolin. Flow birefringence measurements have revealed that with high Mr values (high TMs) partially protect actin filaments from fragmentation by gelsolin, while tropomyosins low (low no significant protection even when the been fully saturated TMs. We also examined effect nonmuscle caldesmon severing gelsolin because 83-kDa stimulates binding cell TMs...
Abstract Using a newly developed method for microfilament isolation (Matsumura, F., Yamashiro-Matsumura, S. and Lin, J. J.-C. (1983) Biol. Chem. 258, 6636-6644), we have analyzed protein composition of microfilaments in normal transformed rat tissue culture cells. They include REF-52 (an established embryo cell line) cells, by DNA viruses (SV40 or adenovirus type 5), kidney cells RNA (Kirsten Rous sarcoma virus). Microfilaments from contain three major tropomyosins (apparent Mr = 40,000,...
Citron kinase is a Rho-effector protein that related to Rho-associated kinases of ROCK/ROK/Rho-kinase family. Both ROCK and citron are suggested play role in cytokinesis. However, no substrates known for kinase. We found phosphorylated regulatory light chain (MLC) myosin II at both Ser-19 Thr-18 vitro. Unlike ROCK, however, did not phosphorylate the binding subunit phosphatase, indicating it does inhibit phosphatase. expression domain resulted an increase MLC di-phosphorylation. Furthermore,...
We have developed a new method for the rapid isolation of tropomyosin-containing microfilaments from cultured cells using anti-tropomyosin monoclonal antibodies. Anti-tropomyosin antibodies induce bundle formation microfilaments, which can be easily collected by low speed centrifugation. Electron microscopic studies isolated show periodic localization tropomyosin along nonmuscle with 33-34 nm repeat. Furthermore, ability to activate Mg2+-ATPase activity skeletal muscle myosin almost same...
Pancreatic ductal adenocarcinoma (PDAC) is often lethal because it highly invasive and metastasizes rapidly. The actin-bundling protein fascin has been identified as a biomarker of advanced PDAC regulates cell migration invasion in vitro. We investigated expression its role progression mice.
Fascin is a 55-58-kDa actin-bundling protein, the actin binding of which regulated by phosphorylation (Yamakita, Y., Ono, S., Matsumura, F., and Yamashiro, S. (1996) J. Biol. Chem. 271, 12632-12638). To understand mechanism fascin-actin interactions, we dissected region its regulatory site human fascin. First, found that C-terminal half constitutes an domain. Partial digestion recombinant fascin with trypsin yielded fragment molecular masses 32, 30, 27 kDa. The 32- 27-kDa fragments purified...
Phosphorylation of the regulatory light chain myosin II (MLC) controls contractility actomyosin in nonmuscle and muscle cells. It has been reported that cdc2 phosphorylates MLC vitro at Ser-1 or Ser-2 Thr-9 which protein kinase C (Satterwhite, L. L., M. J. Lohka, K. Wilson, T. Y. Scherson, Cisek, Corden, D. Pollard. 1992 Cell Biol. 118:595-605). We have examined vivo phosphorylation during mitosis after release mitotic arrest. Phosphate incorporation cells is found to be 6-12 times greater...
Human fascin is an actin-bundling protein that thought to be involved in the assembly of actin filament bundles present microspikes as well membrane ruffles and stress fibers. We have found human phosphorylated vivo upon treatment with 12-O-tetradecanoylphorbol-13-acetate, a tumor promoter. The phosphorylation gradually increased from 0.13 0.30 mol/mol during 2 h treatment, concomitant disappearance fibers, ruffles, microspikes. can also vitro by kinase C at same sites observed judged...
The 55-kD protein is a new actin-bundling purified from HeLa cells (Yamashiro-Matsumura, S., and F. Matsumura, 1985, J. Biol. Chem., 260:5087-5097). We have prepared monoclonal antibodies against the examined its intracellular localization, as well spatial relationships with other components of microfilaments in cultured by double-label immunofluorescence. localization similar to that actin. antibody stained strongly both microspikes stress fibers. was found basal portions extremities while...
From the
To examine functions of tropomyosin in microfilament organization, we have purified and partially separated the multiple isoforms by chromatography on hydroxylapatite.Analyses cross-linked dimers produced air oxidation revealed that all except isoform with apparent M, 35,000 form homodimers.Although these tropomyosins share many properties characteristic tropomyosin, structural analyses at a peptide level immunological shown five can be classified into two groups, i.e. higher (Mr = 40,000,...
Previous results from our laboratory have shown that 1) cultured rat cells contain two classes of tropomyosin (TM), one (high Mr TMs) with higher values and greater affinity for actin than the other (low TMs); 2) presaturation F-actin high TMs, but not low inhibits both actin-severing binding activities gelsolin; 3) nonmuscle caldesmon only enhances inhibitory effects TMs also makes capable inhibiting severing activity gelsolin (Ishikawa, R., Yamashiro, S., Matsumura, F. (1989) J. Biol....
Abstract Dendritic cells (DCs) play central roles in innate and adaptive immunity. Upon maturation, DCs assemble numerous veil-like membrane protrusions, disassemble podosomes, travel from the peripheral tissues to lymph nodes present Ags T cells. These alterations morphology motility are closely linked primary function of DCs, Ag presentation. However, it is unclear how what cytoskeletal proteins control maturation-associated alterations, particular, change cell migration. Fascin1, an...
Cell adhesion to individual macromolecules of the extracellular matrix has dramatic effects on subcellular localization actin-bundling protein fascin and ability cells form stable microspikes. The actin-binding activity is down-regulated by phosphorylation, we used two differentiated cell types, C2C12 skeletal myoblasts LLC-PK1 kidney epithelial cells, examine hypothesis that components fibronectin, laminin-1, thrombospondin-1 differentially regulates phosphorylation. In both treatment with...
At mitosis, focal adhesions disassemble and the signal transduction from is inactivated. We have found that components of including adhesion kinase (FAK), paxillin, p130CAS (CAS) are serine/threonine phosphorylated during mitosis when all three proteins tyrosine dephosphorylated. Mitosis-specific phosphorylation continues past cytokinesis reversed post-mitotic cell spreading. two significant alterations in FAK-mediated mitosis. First, association FAK with CAS or c-Src greatly inhibited,...
Growth cones at the distal tips of growing nerve axons contain bundles actin filaments distributed throughout lamellipodium and that project into filopodia. The regulation bundling by specific binding proteins is likely to play an important role in many growth cone behaviors. Although protein, fascin, has been localized cones, little information available on its functional significance. We used large snail Helisoma determine whether fascin was involved temporal changes during morphogenesis....
A novel human mRNA whose expression is induced over 200-fold in B lymphocytes by latent Epstein-Barr virus (EBV) infection was reverse transcribed, cloned, and sequenced. The predicted to encode a protein containing four peptides which precisely match amino acid sequences from previously identified 55-kDa actin-bundling protein, p55. In vitro translation of the cDNA results binds actin filaments presence purified p55 HeLa cells. undetectable non-EBV-infected B- T-cell lines or myelomonocytic...