A5052797532- Enzyme Structure and Function
- X-ray Diffraction in Crystallography
- Protein Structure and Dynamics
- Crystallization and Solubility Studies
- Glycosylation and Glycoproteins Research
- Mitochondrial Function and Pathology
- Microbial Metabolic Engineering and Bioproduction
- Advanced Fluorescence Microscopy Techniques
- Bacterial Genetics and Biotechnology
- Advanced biosensing and bioanalysis techniques
- Microbial bioremediation and biosurfactants
- ATP Synthase and ATPases Research
- Cancer Research and Treatments
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Genomics and Phylogenetic Studies
- Cellular transport and secretion
- Metabolism and Genetic Disorders
- Drug Transport and Resistance Mechanisms
- Protein Degradation and Inhibitors
- Neuropeptides and Animal Physiology
- Biochemical and biochemical processes
- Peroxisome Proliferator-Activated Receptors
- Receptor Mechanisms and Signaling
St. Jude Children's Research Hospital
2022-2024
University of Connecticut
2017-2019
Indian Institute of Technology Bombay
2013-2017
β-arrestins bind G protein-coupled receptors to terminate protein signaling and facilitate other downstream pathways. Using single-molecule fluorescence resonance energy transfer imaging, we show that β-arrestin is strongly autoinhibited in its basal state. Its engagement with a phosphopeptide mimicking phosphorylated receptor tail efficiently releases the from N domain assume distinct conformations. Unexpectedly, find binding receptor, phosphorylation barcode identical isolated...
Abstract Of the four separate PE biosynthetic pathways in eukaryotes, one occurs mitochondrial inner membrane (IM) and is executed by phosphatidylserine decarboxylase (Psd1). Deletion of Psd1 lethal mice compromises function. We hypothesize that this reflects inefficient import non-mitochondrial into IM. Here, we test re-wiring metabolism yeast re-directing to outer or endomembrane system show can cross IMS both directions. Nonetheless, synthesis IM critical for cytochrome bc 1 complex (III)...
Cardiolipin mediates dynamic receptor-channel interactions within the mitochondrial TIM23 protein import complex.
Pseudomonas putida CSV86, a plasmid-free strain possessing capability to transfer the naphthalene degradation property, has been explored for its metabolic diversity through genome sequencing. The analysis of draft sequence CSV86 (6.4 Mb) revealed presence genes involved in naphthalene, salicylate, benzoate, benzylalcohol, p-hydroxybenzoate, phenylacetate and p-hydroxyphenylacetate on chromosome thus ensuring stability catabolic potential. Moreover, metabolism phenylpropanoid homogentisate,...
Pseudomonas putida CSV86, a soil isolate, preferentially utilizes naphthalene over glucose as source of carbon and energy. We present the draft genome sequence, which is 6.4 Mb in size; analysis suggests chromosomal localization genes coding for utilization. The operons other aromatic compounds might also be annotated another study.
The effective elimination of xenobiotic pollutants from the environment can be achieved by efficient degradation microorganisms even in presence sugars or organic acids. Soil isolate Pseudomonas putida CSV86 displays a unique ability to utilize aromatic compounds prior glucose. draft genome and transcription analyses revealed that glucose uptake benzoate transport metabolism genes are clustered at glc ben loci, respectively, as two distinct operons. When grown on plus benzoate, displayed...
Glucose transport in Pseudomonas putida CSV86 is mediated via a periplasmic glucose-binding protein (GBP)-dependent putative glucose ABC transporter. Here we describe homology model and functional characterization of GBP from (ppGBP). A whole-cell [(14)C]-glucose uptake study revealed that transported by the high-affinity intracellular phosphorylative pathway. ppGBP was cloned, over-expressed Escherichia coli purified to apparent homogeneity. The ppGBPs both E. were found be specific for...
Biochemical data and genomic analysis indicate the involvement of a putative ABC transporter for glucose transport in Pseudomonas putida CSV86. The periplasmic solute binding proteins are known to confer substrate specificity transporters by specifically transferring them their cognate inner membrane assembly. Periplasmic protein from CSV86 (ppGBP) was found be specific. gene encoding ppGBP cloned. Recombinant overexpressed purified homogeneity. recombinant showed activity 752 pmol/mg...
The vast majority of proteins that reside within mitochondria are encoded in nuclear DNA, synthesized on cytosolic ribosomes, and imported into the organelle. Within mitochondrion several types complex machineries mediate sorting, translocation integration incoming polypeptides based their targeting information. Among these translocases, TIM23 Complex mediates import biogenesis most resident proteins, which with cleavable amino‐terminal sequences. is a dynamic multi‐subunit assembly serves...