A5034727479- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- RNA and protein synthesis mechanisms
- Plant nutrient uptake and metabolism
- RNA modifications and cancer
- Light effects on plants
- Genomics and Phylogenetic Studies
- Genomics and Chromatin Dynamics
- Phytoplasmas and Hemiptera pathogens
- RNA Research and Splicing
- Plant Reproductive Biology
University of Tennessee at Knoxville
2014-2024
Howard Hughes Medical Institute
2023
Indiana University Bloomington
2023
Indiana University
2023
The eukaryote-specific ribosomal protein of the small subunit eS6 is phosphorylated through target rapamycin (TOR) kinase pathway. Although this phosphorylation event responds dynamically to environmental conditions and has been studied for over 50 years, its biochemical physiological significance remains controversial poorly understood. Here, we report data from
The translation of mRNA into protein is tightly regulated by the light environment as well circadian clock. Although changes in translational efficiency have been documented at level mRNA-ribosome loading, underlying mechanisms are unclear. reversible phosphorylation ribosomal small subunit 6 (RPS6) has known for forty years, but biochemical significance this event remains unclear to day. Here, we confirm using a clock-deficient strain Arabidopsis thaliana that RPS6 (RPS6-P) controlled diel...
SUMMARY Nucleolus organizer regions (NORs) are eukaryotic chromosomal loci where ribosomal RNA (rRNA) genes clustered, typically in hundreds to thousands of copies. Transcription these rRNA by polymerase I and processing their transcripts results the formation nucleolus, sub‐nuclear domain which ribosomes assembled. Approximately 90 years ago, cytogenetic observations revealed that NORs inherited from different parents an interspecific hybrid sometimes differ morphology at metaphase. Fifty...
Arabidopsis thaliana has two ribosomal RNA (rRNA) gene loci, nucleolus organizer regions NOR2 and NOR4, whose complete sequences are missing in current genome assemblies. Ultralong DNA assembled using an unconventional approach yielded ~5.5- 3.9-Mbp for NOR4 the reference strain, Col-0. The distinct rRNA subtype compositions of NORs enabled positional mapping their active inactive regions, sequencing to identify subtype-specific transcripts subtypes associated with flow-sorted nucleoli....
Essentially all aboveground plant tissues develop from the stem cells in primary shoot apical meristem. Proliferation of cell population Arabidopsis meristem is tightly controlled by a feedback loop formed primarily homeodomain transcription factor WUSCHEL (WUS) and CLAVATA ligand-receptor system. In this study, it shown that mutation translation initiation factor, eIF3h, causes tendency to strikingly enlarged with elevated ectopic expression WUS CLAVATA3 (CLV3). Many mRNAs function...
ABSTRACT In eukaryotes, hundreds of ribosomal RNA (rRNA) genes are clustered at chromosomal loci called nucleolus organizer regions (NORs). Arabidopsis thaliana has two NORs, one on chromosome 2 ( NOR2 ) and the other 4 NOR4 ). Each NOR consists ∼ 400 rRNA gene copies. We recently showed that subtypes map to silenced during development, whereas those active. several DNA methylation mutants Arabidopsis, we show disruption silencing varying degrees. Significantly, highest correlates with a...
Abstract Arabidopsis thaliana has two ribosomal RNA gene loci, nucleolus organizer regions NOR2 and NOR4 , whose complete sequences remain undefined. Ultra-long DNA assembled using an unconventional approach yielded 5.5 3.9 Mbp for (in the reference strain, Col-0), revealing their distinct subtype compositions. sequencing identification of genes associated with flow-sorted nucleoli wild-type or silencing-defective mutant plants shows that most is comprised active whereas most, but not all,...
ABSTRACT The eukaryote-specific ribosomal protein of the small subunit eS6 is phosphorylated through Target rapamycin (TOR) kinase pathway. Although this phosphorylation event responds dynamically to environmental conditions and has been studied for over 50 years, its biochemical physiological significance remains controversial poorly understood. Here we report data from Arabidopsis thaliana , which indicate that plants expressing only a largely phospho-deficient isoform grow essentially...