A5063265997- Aquaculture disease management and microbiota
- Invertebrate Immune Response Mechanisms
- Vibrio bacteria research studies
- Insect symbiosis and bacterial influences
- Mosquito-borne diseases and control
- Bacteriophages and microbial interactions
- Salmonella and Campylobacter epidemiology
- Aquaculture Nutrition and Growth
- Viral gastroenteritis research and epidemiology
- Plant Virus Research Studies
- Bacterial biofilms and quorum sensing
- Animal Genetics and Reproduction
- Viral Infectious Diseases and Gene Expression in Insects
- Identification and Quantification in Food
- Insect Resistance and Genetics
- Glycosylation and Glycoproteins Research
- Animal Virus Infections Studies
Srinakharinwirot University
2013-2023
The gene encoding the capsid protein of Macrobrachium rosenbergii nodavirus (MrNV) was cloned into pGEX-6P-1 expression vector and then transformed Escherichia coli strain BL21. After induction, protein-glutathione-S-transferase (GST-MrNV; 64 kDa) produced. recombinant separated using SDS-PAGE, excised from gel, electro-eluted used for immunization monoclonal antibody (MAb) production. Four MAbs specific to were selected could be detect natural MrNV infections in M. by dot blotting, Western...
Here, two monoclonal antibodies (MAbs) specific to different epitopes on ToxB, a toxin produced by Vibrio parahaemolyticus that causes acute hepatopancreatic necrosis disease (VPAHPND ), were employed develop rapid strip test. One MAb was conjugated colloidal gold bind ToxB at the application pad, and another used capture MAb-protein complexes test line (T) nitrocellulose strip. To validate performance, downstream control (C) of goat anti-mouse immunoglobulin G antibody free conjugate MAb....
The simple immunoprecipitation method was used to isolate tilapia immunoglobulin (Ig) for immunization in order produce monoclonal antibodies (MAbs) specific Ig. First, the antiserum against bovine serum albumin (BSA) prepared by peritoneal injection of BSA into tilapia, and anti-BSA precipitate form Ig/BSA immune complex. complex then injected Swiss mice hybridoma production. After fusion, three clones producing MAbs antibody were selected dot blot Western blot. All (101A, 59G, 11A) bound...
Abstract Vibrio vulnificus is one of the major cause foodborne illness related to seafood consumption. This study aimed develop monoclonal antibodies (MAbs) enable rapid detection and monitoring food contamination this pathogen. Five groups MAbs against V . were generated from a mouse immunized with clinical isolate , heat formalin‐inactivated whole cells. The first two species‐specific recognized all 40 isolates bacterium without cross‐reactivity other Vibiro non‐ species, but bound...
The capsid protein (CP) gene of extra small virus (XSV) expressed in Escherichia coli as a 42 kDa glutathione S-transferase (GST)-fusion (GST-XCP) or 20 His6-fusion (His6-XCP) were purified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), combined, and used to immunize Swiss mice produce monoclonal antibodies (MAbs). Using dot blot, Western immunohistochemistry (IHC) methods, 4 MAbs specific the XSV CP detected freshwater prawn Macrobrachium rosenbergii without...
A portion of the VP26 gene (VP26F109) encoding a structural protein white spot syndrome virus was expressed, purified by SDS-PAGE and used for immunization Swiss mice monoclonal antibody (MAb) production. Three groups MAbs specific to different epitopes on were selected; these can be detect natural WSSV infection in Penaeus vannamei using dot blotting, Western blotting or immunohistochemistry without cross-reaction with other shrimp tissues common viruses. The detection sensitivity ranged...
Abstract A nanogold‐based immunochromatographic strip test (VCG test) for the detection of all 70 isolates Vibrio cholerae including O1, O139, and non‐O1/O139 (NVCs) was developed using two monoclonal antibodies (MAbs), namely VC‐63 VC‐201 , which bound specifically to 10 15 kDa proteins. Direct V . in experimentally spiked fresh seafood samples such as shrimp, blood clam, mussel, oyster could be achieved with sensitivities 7 CFU/ml, similar that dot blotting each MAb. The sensitivity...
Abstract Two monoclonal antibodies (mAbs) specific to Salmonella were produced from a mouse immunized with combination of heat‐killed whole‐cell and formalin‐fixed enterica serovar Enteritidis ( S. Enteritidis) as the antigens. The mAb SE‐13D bound specifically only Enteritidis, while Sal‐06G various members serogroups B, C, D, E, I. Neither demonstrated any cross‐reactivity other bacteria. two mAbs belong IgM class IgG2a subclass, respectively. Using dot‐blot assay, detection sensitivity...