Background: Due to the possible biomedical potential of nanoparticles, titanium dioxide nanoparticles (TiO2 NPs) have received great attention in cancer research. Although selectivity cytotoxicity with TiO2 NPs various cells is clinically significant comparisons and non-cancer been limited. Therefore, we here studied exposure colorectal (CRCs) human umbilical vein endothelial (HUVECs). Methods: After characterization NPs, culture treatment (HCT116, HT29 HUVEC), viability was assessed by MTT...

We studied the functionality of antioxidant system in laboratory rat cardiomyocytes and blood under psycho-emotional stress. It was found that 40-day isolation violation diurnal cycle among animals were accompanied by intensification lipid peroxidation process marked with a reduced activity enzymes, such as catalase superoxide dismutase activity. The results suggested stress oxidative stress, causing reduction intensity energy metabolism cardiomyocytes, which further strengthened fact...

We have studied biochemical changes occurring in brain cell mitochondria of white rats on the background stress induced by 30-day isolation and disruption circadian rhythms. It was ascertained that due to long-lasting stress, there occurs activation oxidative processes as well inhibition anti-oxidant system activity, causing development energy deficiency cells. The above-mentioned become reason for opening mitochondrial permeability transition pore (MPTP), which, its turn, signals start...

We have experimentally demonstrated that the emission of visible light from polymer matrix doped with luminescent dye and gold nanoparticles (GNPs) can be enhanced use surface plasmon coupling. GNPs enhance luminescence intensity nearby because interactions between dipole moments field GNPs. The electric charge on distance molecules a significant effect intensity, this enhancement depends strongly upon excitation wavelength pumping laser source. In particular, by matching frequency to source...

Disturbances in natural Circadian rhythm are well-known stress factors, affecting a range of metabolic pathways the living body including brain. Hence, discovery compounds that could help to prevent and cure adverse changes is very important. One recently discussed substances creatine, believed have anti-stressor properties. Recent paper describes impact intraperitoneally injected creatine (140 mg/kg) into rats with disturbed circadian for an extended period time (30 days). Markedly,...

We have studied the influences that prolonged isolation and disruption of circadian cycle on behavioral activity hormonal status among animals. It has been showed such conditions result in development stress, decreases occur both isoforms rat hippocampus creatine kinase (CK). established kinetic parameters (Vmax, Km) this hippocampal enzyme nature modifications produced by protocol. suggested disrupted natural lower CK-BB animals due to structural changes a decrease ATP substrates. Lowered...

Parkinson's disease (PD) is the second most prevalent neurodegenerative disorder, following Alzheimer's disease, impacting approximately 1% of individuals aged 60 and older.It characterized by degeneration dopaminergic neurons in SNpc, resulting both motor non-motor symptoms.Current treatments are limited, PD's molecular basis involves alpha-synuclein aggregation, ubiquitination dysregulation, oxidative stress.Nicotine, found tobacco, has been associated with reduced PD risk epidemiological...

Distribution of the Mg²⁺-dependent, HCO₃⁻-stimulated ATPase (HCO₃⁻-ATPase) was investigated in sub-cellular fractions tissue human thyroid gland. It found that especially high enzymatic activity is characteristic mitochondria and endoplasmic reticulum. Correlation between various pathologies HCO₃⁻-ATPase demonstrated. The evaluated using difference active passive ATPase. During development pathologies, gland showed uneven alterations activity. Intriguingly, enzyme kinetic parameters are also...

We studied the functionality of antioxidant system in laboratory rat cardiac muscle cells and blood under psycho-emotional stress.It was found that 40-day isolation violation diurnal cycle among animals were accompanied by intensification lipid per oxidation process shown a rise quantity one primary final products this -diene conjugates thiobarbituric acid active compounds, including malonedialdehydes marked with reduced activity enzymes, such as catalase superoxide dismutase activity.Based...

The functional states of pro- and antioxidant systems in blood heart muscle cells rats with long-term emotional stress have been studied. It has shown that daily rhythm disorders produce psycho-emotional animals that, this is accompanied by quantitative changes physiological parameters hormones the blood. In present study, it was observed such increased lipid peroxidation cells. Also, activities enzymes, superoxide dismutase, catalase were diminished, indicating deterioration system....

For the last few decades, aggression has been linked to various modifications in cellular metabolic pathways.It also shown that patients undergoing extended creatine (Cr) treatment display increased aggressiveness.However, molecular mechanisms underlying a correlation between two phenomena have not sufficiently identified.To gain deeper understanding of changes resulting from supplementation Cr into non-aggressive animals, we compared some behavioral and biochemical characteristics...

Plasma membrane of human thyroid gland, apart from the transport Mg-independent HCO3—ATPase, has been found to contain also HCO3--ATPase ecto-ATPase type, whose activity is not conditioned by Mg-ion and with substrate in form free ATP (ecto-HCO3--ATPase). Activity such kind both healthy cells those affected carcinoma. However, latter cells, this characteristic enzyme much higher than norm. We studied certain kinetic properties HCO3--ATPase, namely relation its quantitative content HCO...