A5090858552- Neuroscience and Neuropharmacology Research
- Mitochondrial Function and Pathology
- Photoreceptor and optogenetics research
- Neural dynamics and brain function
- Alzheimer's disease research and treatments
- Diet and metabolism studies
- Memory and Neural Mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Ion channel regulation and function
- Bone and Dental Protein Studies
- Anesthesia and Neurotoxicity Research
- Neurogenesis and neuroplasticity mechanisms
- Retinal Development and Disorders
- Photochromic and Fluorescence Chemistry
- Supramolecular Self-Assembly in Materials
- Receptor Mechanisms and Signaling
- Neuroscience and Neural Engineering
- Genetics and Neurodevelopmental Disorders
- Advanced Memory and Neural Computing
- Amino Acid Enzymes and Metabolism
- Gender Roles and Identity Studies
- Epilepsy research and treatment
University of Geneva
2013-2015
McGill University Health Centre
2011
Montreal General Hospital
2011
University of Lausanne
2004-2008
Glutamate-evoked Na+ increase in astrocytes has been identified as a signal coupling synaptic activity to glucose consumption. Astrocytes participate multicellular signaling by transmitting intercellular Ca2+ waves. Here we show that waves are also evoked activation of single cultured cortical mouse parallel with waves; however, there spatial and temporal differences. Indeed, maneuvers inhibit inhibition the Na+/glutamate cotransporters or enzymatic degradation extracellular glutamate...
Neurons recruit numerous mechanisms to facilitate the development of synaptic connections. However, little is known about activity-dependent that control timing and fidelity this process. Here we describe a novel pathway used by neurons regulate glutamate receptors at maturing central synapses. This relies on communication between astrocytes ability release factor SPARC (secreted protein, acidic rich in cysteine). expression dynamically regulated plays critical role determining level AMPARs....
Astrocytes show a complex structural and physiological interplay with neurons respond to neuronal activation <i>in vitro</i> vivo</i> intracellular calcium elevations. These changes enable astrocytes modulate synaptic transmission plasticity through various mechanisms. However, the response pattern of single depolarization events still remains unresolved. This information is critical for fully understanding coordinated network neuron–glial signaling in brain. To address this, we developed...
Several gene mutations linked to intellectual disability in humans code for synaptic molecules implicated small GTPase signaling. This is the case of Rac/Cdc42 effector p21-activated kinase 3 (PAK3). The mechanisms responsible defects and consequences mutation on development wiring brain networks remain unknown. Here we show that expression PAK3 mutants, suppression PAK3, or inhibition function rat hippocampal slice cultures interfere with activity-mediated spine dynamics. Inhibition...
The proper formation and maintenance of functional synapses in the central nervous system (CNS) requires communication between neurons astrocytes ability to release neuromodulatory molecules. Previously, we described a novel role for astrocyte-secreted matricellular protein SPARC (Secreted Protein, Acidic Rich Cysteine) regulating α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs) plasticity at developing synapses. is highly expressed by microglia during CNS development...
Abstract Astrocytes can experience large intracellular Na + changes following the activation of ‐coupled glutamate transport. The present study investigated whether cytosolic are transmitted to mitochondria, which could therefore influence their function and contribute overall regulation. Mitochondrial (Na ) were monitored using ‐sensitive fluorescent probe CoroNa Red (CR) in intact primary cortical astrocytes, as opposed classical isolated mitochondria preparation. mitochondrial...
Glial cells play multiple, diverse roles in the central nervous system (CNS), ranging from basal support of neuronal function to close partnership with synapse. Growing experimental evidence shows importance glia for proper brain functioning and their involvement injury disease. Astrocytes are most intriguing among glial family. It is well known that they provide energetic substrates neurons, take up neurotransmitters, maintain ion homeostasis. Recent research has revealed can also release...
Neuroscience studies require technologies able to deliver compounds with both scale and timing compatibility morphological physiological synaptic properties. In this light, two-photon flash photolysis has been extensively used successfully apply glutamate or other neurotransmitters at the level. However, set of commercially available caged is restricted incompatible demanding high cell specificity. The gain in specificity especially relevant challenging when studying neuron-glia interactions...
Abstract Alzheimer's disease (AD) is becoming increasingly prevalent worldwide. It represents one of the greatest medical challenges as no pharmacologic treatments are available to prevent progression. Astrocytes play crucial functions within neuronal circuits by providing metabolic and functional support, regulating interstitial solute composition, modulating synaptic transmission. In addition these physiological functions, growing evidence points an essential role astrocytes in...
Abstract Alzheimer’s disease (AD) is becoming increasingly prevalent worldwide. It represents one of the greatest medical challenge as no pharmacologic treatments are available to prevent progression. Astrocytes play crucial functions within neuronal circuits by providing metabolic and functional support, regulating interstitial solute composition, modulating synaptic transmission. In addition these physiological functions, growing evidence points an essential role astrocytes in...