Abstract Metastasis remains the leading cause (90%) of cancer-related mortality, especially in metastatic triple-negative breast cancer (TNBC). Improved understanding molecular drivers cascade is crucial, to find accurate prognostic markers for invasiveness after chemotherapy treatment. Current treatments include doxorubicin and paclitaxel, inducing various effects, such as unfolded protein response (UPR). The key regulator UPR 78-kDa glucose-regulated (GRP78), which associated with disease,...

// Annat Raiter 1, * , Rinat Yerushalmi 2, Britta Hardy 1 Felsenstein Medical Research Center, Tel Aviv University School of Medicine, Rabin Petach Tikva, 49100, Israel 2 Oncology Institute, These authors contributed equally to this work Correspondence to: Hardy, e-mail: bhardy@post.tau.ac.il Keywords: triple negative breast cancer cells, drug induced cell surface GRP78, apoptosis Received: July 16, 2014      Accepted: October 05, Published: 24, 2014 ABSTRACT Breast...

A preliminary study investigating immunotherapy strategies for aggressive triple negative breast cancer (TNBC) revealed an overexpression of genes involved in the release extracellular vesicles (EVs). Proteins expressed by EVs play a role reprogramming tumor microenvironment and impeding effective responses to immunotherapy. Galectin 3 (Gal3), found space cells, downregulates T-cell receptor expression. Gal3 binds several receptors, including CD45, which is required activation. Previously,...

Impaired angiogenesis is one of the features ischemic diseases. We have previously identified, by screening a phage display peptide library, that induces in endothelial cells under hypoxic conditions binding cell’s membrane heat shock protein GRP78. Protein data base search identified 4 amino acids (HWRR) synthetic present on ADAM15 metalloprotease domain, considered to be involved neovascularization. Three peptides were synthesized according sequence placing HWRR at different positions....

The 78-kDa glucose-regulated protein (GRP78) is a stress induced heat shock which, under limiting conditions, functions as cell surface signaling receptor. Tumor cells are considered to be subjected physiologically stressful microenvironment due their excessive growth. role of GRP78 in tumor survival has been notable interest. present study aimed assess the potential prognostic and predictive value expression breast cancer cells. Cell cytoplasmic was examined by immunohistochemical staining...

Background The identification of new biomarkers is required for early diagnosis colorectal carcinoma patients (CRC), since about 20% these are initially diagnosed with a distant metastatic disease. GRP78, heat shock protein, functions also as cell surface signaling receptor cells under physiological stress. GRP78 was found to be expressed on the various tumor cells. presence autoantibodies in cancer patient's serum correlated poor prognosis. In this study we aimed identify anti-GRP78...

Breast cancer subtypes have not shown significant response to current immunomodulatory therapies. Although most are treatable, triple negative breast (TNBC), an aggressive highly metastatic cancer, comprising 10–20% of cancers, remains unmet medical need. New strategies needed in order overcome flaws the responsiveness TNBC Our aims were: first, determine efficacy a novel peptide, C24D, on and second, elucidate molecular mechanism by which C24D induces immune-modulating tumor killing. Using...

Background: The emergence of new SARS-CoV-2 variants, which evade immunity, has raised the urgent need for multiple vaccine booster doses vulnerable populations. In this study, we aimed to estimate BNT162b2 effectiveness against spread coronavirus variants in a hemodialysis population. Methods: We compared humoral and cell-mediated immunity 100 dialysis patients 66 age-matched volunteers, before 2-3 weeks following first dose. Participants were assessed anti-spike (RBD) antibody titer,...

Monoclonal antibodies were raised against Daudi B-lymphoblastoid cell line membranes. An mAb (BAT) was selected for its ability to stimulate human and murine lymphocyte proliferation. BAT induced cytotoxicity in lymphocytes natural killer cell-sensitive -resistant tumor lines. A single intravenous administration of mice that had been inoculated with various tumors (e.g., B16 melanoma, 3LL carcinoma, methylcholanthrene fibrosarcoma) resulted striking antitumor effects as manifested by...

To achieve a cure for metastatic breast cancer, further understanding of molecular drivers the cascade is essential. Currently, chemotherapy regimens include doxorubicin and paclitaxel which act in part by inducing unfolded protein response (UPR). The master regulator UPR, glucose regulated 78 (GRP78), localizes on surface tumor cells associated with disease. Cyclic AMP responsive element binding 3-like 1 (CREB3L1), member cancer metastasis suppressor that acts cyclic to promote expression...

CD45, the predominant transmembrane tyrosine phosphatase in leukocytes, is required for efficient induction of T cell receptor signaling and activation. We recently reported that CD45-intracellular signals peripheral blood mononuclear cells (PBMCs) triple negative breast cancer (TNBC) patients are inhibited. also C24D, an immune modulating therapeutic peptide, binds to CD45 on immune-suppressed resets functionality system via pathway. Various studies have demonstrated viruses can interfere...

Intravenous immunoglobulins (IVIg) preparations can be beneficial therapeutic agents for the treatment of tumor metastases as has been shown in both human and animal studies. Operating mechanisms have not yet completely elucidated. Some proposed entail stimulation production IL-12, a cytokine that exhibits anti-angiogenic activities, well inhibition endothelial cells proliferation vascular growth factor (VEGF) secretion. The aim present study was to investigate whether an IVIg preparation...

Abstract Background Breast cancer treatment is tailored to the specific subtype. Often, systemic given prior surgery. Chemotherapy induces significant endoplasmic reticulum (ER) stress-mediated cell death and upregulation of 78-kDa glucose-regulated protein (GRP78). We hypothesized that chemotherapy ER stress not only in tumor tissue but also immune cells, which may affect response anti-cancer treatment. Methods determined surface expression GRP78 on 15 different peripheral blood mononuclear...

BAT is a monoclonal antibody produced against membranes of Daudi cells that induces anti-tumor activity in mice variety solid murine and human tumors, mediated by its immune stimulatory properties on lymphocytes. The present study analyzes the effect leukemia/lymphoma using BCL1 model BALB/C mice. binds to leukemia recognizes 48 kDa protein similar antigen cells. Mice inoculated with were treated either direct injections or adoptive transfer lymphocytes from BAT-injected Administration was...