A5059049743- Photosynthetic Processes and Mechanisms
- DNA Repair Mechanisms
- Microtubule and mitosis dynamics
- Cell Image Analysis Techniques
- Medical Imaging Techniques and Applications
- Advanced Fluorescence Microscopy Techniques
- Chromosomal and Genetic Variations
- Radiopharmaceutical Chemistry and Applications
- Photoacoustic and Ultrasonic Imaging
- Reproductive Biology and Fertility
- Cancer therapeutics and mechanisms
- Advanced MRI Techniques and Applications
- Protein Structure and Dynamics
- Advanced NMR Techniques and Applications
- DNA and Nucleic Acid Chemistry
- Protist diversity and phylogeny
University of North Carolina at Chapel Hill
2015-2021
Fluorescence microscopy is a powerful approach for studying subcellular dynamics at high spatiotemporal resolution; however, conventional fluorescence techniques are light-intensive and introduce unnecessary photodamage. Light-sheet (LSFM) mitigates these problems by selectively illuminating the focal plane of detection objective using orthogonal excitation. Orthogonal excitation requires geometries that physically limit numerical aperture (NA), thereby limiting both light-gathering...
The size of mitotic chromosomes is coordinated with cell in a manner dependent on nuclear trafficking. In this study, we conducted an RNA interference screen the Caenorhabditis elegans nucleome strain carrying exceptionally long chromosome and identified centromere-specific histone H3 variant CENP-A DNA decatenizing enzyme topoisomerase-II (topo-II) as candidate modulators size. holocentric organism C. elegans, positioned periodically along entire length chromosomes, mitosis, these genomic...
Understanding how the crowded and complex cellular milieu affects protein stability dynamics has only recently become possible by using techniques such as in-cell nuclear magnetic resonance. However, combination of stabilizing destabilizing interactions makes simple predictions difficult. Here we show potential Danio rerio oocytes an resonance model that can be widely used to measure dynamics. We demonstrate in eukaryotic oocytes, which are 3–6-fold less than other cell types, attractive...
Fluorescence microscopy is a powerful approach for studying sub-cellular dynamics at high spatiotemporal resolution; however, conventional fluorescence techniques are light-intensive and introduce unnecessary photodamage. Light sheet (LSFM) mitigates these problems by selectively illuminating the focal plane of detection objective using orthogonal excitation. Orthogonal excitation requires geometries that physically limit numerical aperture (NA), thereby limiting both light-gathering...