A5020523350- Banana Cultivation and Research
- Plant Stress Responses and Tolerance
- Plant tissue culture and regeneration
- CRISPR and Genetic Engineering
- Plant Gene Expression Analysis
- Chromosomal and Genetic Variations
- Chronic Lymphocytic Leukemia Research
- Advanced biosensing and bioanalysis techniques
- Plant biochemistry and biosynthesis
- Transgenic Plants and Applications
- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Plant Pathogens and Fungal Diseases
- GABA and Rice Research
- Plant-Microbe Interactions and Immunity
- Pancreatic function and diabetes
- Immunodeficiency and Autoimmune Disorders
- Plant and soil sciences
Guangdong Academy of Agricultural Sciences
2015-2024
Ministry of Agriculture and Rural Affairs
2019-2020
Institute of Fruit Tree and Tea Science
2020
Hunan Agricultural University
2011-2019
Banana is an important staple crop and widely grown in more than 130 countries around the world. Plant height one of major characteristics crops (Wang et al., 2018); however, commercial banana varieties 2 m, higher are frequently challenged by weak lodging severe damages caused typhoons storms. Dwarf suitable for mechanized plant maintenance fruit harvesting (Dash Rai, 2016); thus, development semi-dwarf dwarf to current farming systems. Gibberellin (GA) most determinants (Sasaki 2002),...
Abstract Background To date, CRISPR/Cas9 RNP editing tools have not been applied to the genetic modification of banana. Here, establishment a PEG-mediated banana protoplast transformation system makes it possible build an efficient DNA-free method for site-directed mutagenesis system. Results Protoplasts constitute versatile platform transient expression in plant science. In this study, we established This was further optimized successfully delivering and CRISPR/Cas12a plasmids...
Current banana cultivation is facing a serious threat known as Panama disease or Fusarium wilt, which caused by the oxysporum (Dale et al., 2017). To date, development of wilt-resistant still faces challenges various sexual reproduction barriers, such high sterility, complex genetic background, polypoid, and parthenogenesis (Ghag 2014), making it extremely difficult to develop new disease-resistant bananas via cross-breeding (Czislowski 2018). In recent years, host-induced gene silencing...
Tobacco ( Nicotiana tabacum L.) is an important industrial crop, which sensitive to chilling stress. seedlings that have been subjected stress readily flower early, seriously affects the yield and quality of their leaves. Currently, there has progress in elucidating molecular mechanisms by tobacco responds However, little known about phosphorylation mediated chilling. In this study, transcriptome, proteome phosphoproteome were analyzed elucidate responses shoot root (4 °C for 24 h). A total...
Abstract Low temperature is one of the key environmental stresses, which greatly affects global banana production. However, little known about phosphoproteomes in Musa spp . and their regulatory roles response to cold stress. In this study, we conducted a comparative phosphoproteomic profiling cold-sensitive Cavendish Banana relatively tolerant Dajiao under Phosphopeptide abundances five phosphoproteins involved MKK2 interaction network, including MKK2, HY5, CaSR, STN7 kinesin-like protein,...
Banana is an important tropical fruit with high economic value. One of the main species (cultivar Cavendish) susceptible to low temperature, while another close relative specie (Dajiao), has considerably higher cold tolerance. We have reported that some membrane proteins appear involved in tolerance Dajiao via antioxidation mechanism. To investigate early stress response Dajiao, here we applied comparative proteomics analysis for both cold-sensitive Cavendish and cold-tolerant subjected at...
Heat stress is a prevalent factor that significantly damages crops, especially with the ongoing global warming and increasing frequency of extreme weather events. Tobacco particularly sensitive to temperature fluctuations, experiencing reduced yield quality under high temperatures. However, underlying molecular mechanisms heat resistance in tobacco remain poorly understood. This study comprehensively analyzed biochemical, transcriptomic, metabolomic responses on root shoot cultivar K326...
Abstract Banana ( Musa spp.) is an important tropical crop. industry under biotic and abiotic stresses such as Fusarium wilt, typhoon, cold stress. Genetic engineering offers a powerful strategy to create germplasm of banana with enhanced resistance. The safety genetically modified organisms has become bottleneck restricting the popularization application technology. In this study, candidate promoter, LEAFY (LFY) for expression flower initiation in Arabidopsis , was cloned constructed...
Abstract Background: To date, CRISPR/Cas9 RNPs editing tools have not been applied to genetic modification of banana. Here, the establishment PEG-mediated banana protoplast transformation system makes it possible build an efficient DNA- free method for site-directed mutagenesis system. Results: Protoplasts are a versatile platform transient expression in plant science. In this study, we established This was further optimized successfully delivering plasmids and CRISPR/Cas12a ,...