A5006148882- Cellular transport and secretion
- Endoplasmic Reticulum Stress and Disease
- Fungal and yeast genetics research
- Glycosylation and Glycoproteins Research
- Lysosomal Storage Disorders Research
- Medical Imaging Techniques and Applications
- Mitochondrial Function and Pathology
- Lipid metabolism and biosynthesis
- Sphingolipid Metabolism and Signaling
- Cancer, Hypoxia, and Metabolism
Cornell University
2024
Kagawa University
2021-2022
MCCs are linear invaginations of the yeast plasma membrane that form stable microdomains. Although over 20 proteins localized in MCCs, it is not well understood how these coordinately maintain normal MCC function. Pil1 a core eisosome protein and responsible for MCC-invaginated structures. In addition, six-tetraspan (6-Tsp) classified into two families, Sur7 family Nce102 family. To understand coordinated function proteins, single multiple deletion mutants 6-Tsp were generated their...
Saccharomyces cerevisiae LIP1 encodes a regulatory subunit that forms complex with the ceramide synthase catalytic subunits, Lag1/Lac1, which is localized on membrane of endoplasmic reticulum. To understand underlying mechanism sphingolipid biosynthesis, we generated strains upon replacing chromosomal promoter Tet-off promoter, enables expression in Dox-dependent manner. The lip1-1 strain, obtained through substitution, exhibits severe growth inhibition and remarkable decrease synthesis...
ABSTRACT The oligosaccharide needed for protein N -glycosylation is assembled on a lipid carrier via multi-step pathway. Synthesis initiated the cytoplasmic face of endoplasmic reticulum (ER) and completed luminal side after transbilayer translocation heptasaccharide intermediate. More than 30 Congenital Disorders Glycosylation (CDGs) are associated with this pathway, including RFT1-CDG which results from defects in membrane Rft1. Rft1 essential viability yeast mammalian cells was proposed...
The oligosaccharide needed for protein N-glycosylation is assembled on a lipid carrier via multistep pathway. Synthesis initiated the cytoplasmic face of endoplasmic reticulum (ER) and completed luminal side after transbilayer translocation heptasaccharide intermediate. More than 30 congenital disorders glycosylation (CDGs) are associated with this pathway, including RFT1-CDG which results from defects in membrane Rft1. Rft1 essential viability yeast mammalian cells was proposed as...
Abstract Global control for the synthesis of lipids constituting a bilayer cell membranes is known to be with small number transcription factors called master transcriptional regulators, which target wide range genes encoding lipid metabolism enzymes and/or their regulators. Although regulators glycerophospholipids and sterols have been identified in both yeast mammals, this aspect sphingolipid not yet understood. In present study, we C2H2-type zinc finger factor, Com2, as regulator budding...