Mitotic rounding during cell division is critical for preventing daughter cells from inheriting an abnormal number of chromosomes, a condition that occurs frequently in cancer cells. Cells must significantly expand their apical area and transition polygonal to circular shape achieve robust mitotic epithelial tissues, which where most cancers initiate. However, how mechanically regulate within packed tissues unknown. Here, we analyze using newly developed multi-scale subcellular element...

Epithelial sheets define organ architecture during development. Here, we employed an iterative multiscale computational modeling and quantitative experimental approach to decouple direct indirect effects of actomyosin-generated forces, nuclear positioning, extracellular matrix, cell-cell adhesion in shaping Drosophila wing imaginal discs. Basally generated actomyosin forces generate epithelial bending the disc pouch. Surprisingly, acute pharmacological inhibition ROCK-driven contractility...

A nonlinear computational model, based on solving the Navier-Stokes equation, is used to study motion of a 5 MW spar buoy floating wind turbine in moderate and extreme sea states with irregular waves. The main advantages using current model are that there no limitation platform motion, hydrodynamic loads do not rely experimental data, can be predicted. work extends previously developed for regular periodic waves tension leg turbine. Free decay tests performed, pitch, heave, surge natural...

The dynamic motion of floating wind turbines is studied using numerical simulations. full three-dimensional Navier–Stokes equations are solved on a regular structured grid level set method for the free surface and an immersed boundary turbine platform. tethers, tower, nacelle, rotor weight included reduced-order models, resulting in efficient approach that can handle nearly all nonlinear hydrodynamic forces platform, while imposing no limitation platform motion. Wind speed assumed constant,...

Stem cell maintenance in multilayered shoot apical meristems (SAMs) of plants requires strict regulation growth and division. Exactly how the complex milieu chemical mechanical signals interact central region SAM to regulate division plane orientation is not well understood. In this paper, simulations using a newly developed multiscale computational model are combined with experimental studies suggest test three hypothesized mechanisms for direction anisotropic expansion corpus. Simulations...

Budding yeast, Saccharomyces cerevisiae, serves as a prime biological model to study mechanisms underlying asymmetric growth. Previous studies have shown that prior bud emergence, polarization of conserved small GTPase Cdc42 must be established on the cell membrane budding yeast. Additionally, such contributes delivery wall remodeling enzymes and hydrolase from cytosol through membrane, change mechanical properties wall. This leads hypothesis its associated proteins at least indirectly...

In the present paper, a hybrid Computational Fluid Dynamics (CFD) and Boundary Integral Element Method (BIEM) framework is developed in order to study response of moored Multibody wave Energy Device (MED) panchromatic sea state. The relevant results are surge heave responses MED. Numerical Analysis Framework (NAF) includes two different models; first model uses Navier-Stokes equations describe flow field solved with an in-house CFD code quantify viscous damping effect, while second...

Abstract The exact mechanism controlling cell growth remains a grand challenge in developmental biology and regenerative medicine. Drosophila wing disc tissue serves as an ideal biological model to study mechanisms involved regulation. Most existing computational models for studying focus specifically on either chemical signals or mechanical forces. Here we developed multiscale chemical-mechanical investigate the regulation based dynamics of morphogen gradient. By comparing spatial...

The dynamic motion of floating wind turbines is studied using computational simulations. full three-dimensional Navier-Stokes equations are solved on a regular structured grid, level set method for the free surface and modified immersed boundary to model turbine platform. tethers, tower, nacelle rotor weight include reduced order models, resulting in an efficient numerical approach. Wind modeled as constant thrust force. Other aerodynamic loading, gyroscopic effects, aeroelastic effects not...

A computational fluid dynamics (CFD) based numerical wave tank (NWT) is developed and verified to study load effects on fixed free floating offshore structures. The model solving Navier–Stokes equations a structured grid, level set method for tracking the surface, an immersed boundary studying wave–structure interaction. This paper deals with establishing verifying CFD-based NWT. Various concerns that arise during this establishment are discussed, namely of reflection which might affect...

Abstract Mitotic rounding during cell division is critical for preventing daughter cells from inheriting an abnormal number of chromosomes, a condition that occurs frequently in cancer cells. Cells must significantly expand their apical area and transition polygonal to circular shape achieve robust mitotic epithelial tissues, which where most cancers initiate. However, how mechanically regulate within packed tissues unknown. Here, we analyze using newly developed multi-scale subcellular...

Abstract Reproducibility is a significant challenge in (epi)genomic research due to the complexity of experiments composed traditional biochemistry and informatics. Recent advances have exacerbated this as high-throughput sequencing data generated at an unprecedented pace. Here, we report development P latform for E pi- G enomic R esearch (PEGR), web-based project management platform that tracks quality controls from conception publication-ready figures, compatible with multiple assays...

The ability to aggregate experimental data analysis and results into a concise interpretable format is key step in evaluating the success of an experiment. This critical determines baselines for reproducibility requirement dissemination. However, practice it can be difficult consolidate analyses that encapsulates broad range datatypes available life sciences. We present STENCIL, web templating engine designed organize, visualize, enable sharing interactive visualizations. STENCIL leverages...

Abstract Epithelial sheets play important roles in defining organ architecture during development. Here, we employed an iterative experimental and multi-scale computational modeling approach to decouple direct indirect effects of actomyosin-generated forces, nuclear positioning, extracellular matrix (ECM), cell-cell adhesion shaping Drosophila wing imaginal discs, a powerful system for elucidating general principles epithelial morphogenesis. Basally generated actomyosin forces are found...

The dynamic motion of floating wind turbines is studied using numerical simulations.The full Navier-Stokes equations are solved on a regular structured grid, level set method for the free surface and an immersed boundary to model turbine platform.The response tension leg platform moderate amplitude waves examined effect key design parameter quantified in this initial study.It shown that pitch surge amplitudes larger than heave motions, increased ballast weight helps stabilize motions reduce...