A5025232317- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Evolution and Genetic Dynamics
- CRISPR and Genetic Engineering
- Monoclonal and Polyclonal Antibodies Research
- Microbial Metabolic Engineering and Bioproduction
- Advanced Fluorescence Microscopy Techniques
- RNA modifications and cancer
- Genomics and Phylogenetic Studies
- Gastrointestinal motility and disorders
- Enzyme Structure and Function
- Heat shock proteins research
- Cancer Research and Treatments
- Congenital gastrointestinal and neural anomalies
- Protein Structure and Dynamics
- Mass Spectrometry Techniques and Applications
- Genetics, Bioinformatics, and Biomedical Research
- Diverticular Disease and Complications
- Molecular Biology Techniques and Applications
- Cell Image Analysis Techniques
- Viral gastroenteritis research and epidemiology
- Genetic diversity and population structure
- Algal biology and biofuel production
- Advanced Statistical Process Monitoring
Uppsala University
2005-2024
Small molecule neurotransmitters are essential for the function of nervous system, and neurotransmitter imbalances often connected to neurological disorders. The ability quantify such is important provide insights into biochemical mechanisms underlying disorder. This proof-of-principle study presents online quantification small neurotransmitters, specifically acetylcholine, γ-aminobutyric acid (GABA) glutamate, in rat brain tissue sections using nanospray desorption electrospray ionization...
Experimental evolution under controlled laboratory conditions is becoming increasingly important to address various evolutionary questions, including, for example, the dynamics and mechanisms of genetic adaptation different growth stress conditions. In such experiments, mutations typically appear that increase fitness tested (medium adaptation), but are not necessarily interest specific research question. Here, we have identified appeared during serial passage E. coli S. enterica in four...
Coral reefs are colored by eukaryotic chromoproteins (CPs) that homologous to green fluorescent protein. CPs differ from proteins (FPs) intensely absorbing visible light give strong colors in ambient light. This endows with certain advantages over FPs, such as instrument-free detection uncomplicated ultra-violet damage or background fluorescence, efficient Förster resonance energy transfer (FRET) quenching, and photoacoustic imaging. Thus, have found utility genetic markers teaching,...
Abstract Ribosome mediated mRNA translation is central to life. The cycle of translation, however, has been characterized mostly using reconstituted systems, with only few techniques applicable for studies in the living cell. Here we describe a live-cell ribosome-labeling method, which allows us characterize whole processes finding and translating an mRNA, single-molecule tracking techniques. We find that more than 90% both bacterial ribosomal subunits are engaged at any particular time, 30S...
The distribution of fitness effects mutations is a factor fundamental importance in evolutionary biology. We determined the 510 mutants that each carried between 1 and 10 (synonymous nonsynonymous) hisA gene, encoding an essential enzyme l-histidine biosynthesis pathway Salmonella enterica. For full set mutants, was bimodal with many apparently neutral lethal mutations. subset 81 single, nonsynonymous most appeared at high expression levels, whereas low levels only few were neutral....
Mechanistic details of the signal recognition particle (SRP)-mediated insertion membrane proteins have been described from decades in vitro biochemical studies. However, dynamics pathway inside living cell remain obscure. By combining vivo single-molecule tracking with numerical modeling and simulated microscopy, we constructed a quantitative reaction-diffusion model SRP cycle. Our results suggest that SRP-ribosome complex finds its target, membrane-bound translocon, through combination...
Abstract The bacterial chaperone Trigger factor (TF) binds to ribosome-nascent chain complexes (RNCs) and co-translationally aids the folding of proteins in bacteria. Decades studies have given a broad, but often conflicting, description substrate specificity TF, its RNC-binding dynamics, competition with other factors, such as Signal Recognition Particle (SRP). Previous kinetics experiments were conducted on stalled RNCs reconstituted systems, consequently, may not represent interaction TF...
When a new activity evolves by changes in pre-existing enzyme this is likely to reduce the original activity, generating functional trade-off. The properties of trade-off will affect continued evolution both functions. If strong, gene duplication and subsequent divergence would be favored whereas if weak bi-functional could evolve that performs We previously showed when HisA was evolved under selection for TrpF functions, mainly proceeded via duplication-divergence specialization, implying...
The bacterial chaperone Trigger factor (TF) binds to ribosome-nascent chain complexes (RNCs) and cotranslationally aids the folding of proteins in bacteria. Decades studies have given a broad, but often conflicting, description substrate specificity TF, its RNC-binding dynamics, competition with other factors, such as Signal Recognition Particle (SRP). Previous kinetics experiments were commonly conducted on stalled RNCs reconstituted systems, consequently, may not be representative...
Bacteria are known to display extensive metabolic diversity and many studies have shown that they can use an repertoire of small molecules as carbon- energy sources. However, it is less clear what extent a bacterium expand its existing capabilities by acquiring mutations that, for example, rewire pathways. To investigate this capability potential evolution novel phenotypes, we sampled large populations mutagenized Salmonella enterica select very rare mutants grow on minimal media containing...
ABSTRACT Ribosome mediated mRNA translation is central to life as we know it. The cycle of has, however, not been characterized in a living cell. Here have developed live-cell ribosome-labeling method, which allows us characterize the whole processes finding an and translating it, using single-molecule tracking techniques. We find that more than 90% both bacterial ribosomal subunits are engaged elongation at any particular time, neither subunits, general, continues from one open reading...