A5092858805- Cell Image Analysis Techniques
- Optical Coherence Tomography Applications
- Retinal Imaging and Analysis
- Photoacoustic and Ultrasonic Imaging
- Single-cell and spatial transcriptomics
Polytechnique Montréal
2023-2024
Optical coherence tomography (OCT) has shown an affinity for imaging white matter using intrinsic signals. Combined with automated vibratome and mosaic imaging, serial blockface histology (SBH) can yield whole-brain images at high resolution. A current drawback of SBH is the lack real-time information that complicates localization brain structures during imaging. To address this, imaged slices be registered to a pre-existing 3D volume provide more contextual acquisition. image registration...
Previous studies have shown that the optical coherence tomography (OCT) signal in white matter (WM) is affected by WM fiber bundles orientation with respect to microscope's axis. In this paper, we aim exploit contrast mechanism generate a multi-orientation representation of microstructure whole mouse brains. To achieve this, serial blockface histology set-up has been developed combined spectral domain OCT equipped long-range 10x magnification objective, achieving near isotropic resolution 3...
Abstract Neurovascular interactions (NVIs) are critical in establishing vascular patterning, barrier function, and regulating cerebral blood flow, thereby maintaining neuronal homeostasis brain health. While developmental programs activity play a central role cortex angiogenesis during postnatal period, whether cellular heterogeneity causes region-specific angiogenic regulation remains unknown. Here, we combine spatial endothelial single-cell RNAseq (scRNAseq) transcriptomic analysis to...