A5002750439- Allergic Rhinitis and Sensitization
- Food Allergy and Anaphylaxis Research
- Contact Dermatitis and Allergies
- Asthma and respiratory diseases
- Monoclonal and Polyclonal Antibodies Research
- Plant Reproductive Biology
- Dermatology and Skin Diseases
- Essential Oils and Antimicrobial Activity
- Parasitic infections in humans and animals
- Immune Cell Function and Interaction
- Entomological Studies and Ecology
- Congenital Anomalies and Fetal Surgery
- Parasitic Infections and Diagnostics
- Protein purification and stability
- T-cell and B-cell Immunology
- Fuel Cells and Related Materials
- Turfgrass Adaptation and Management
- Diphtheria, Corynebacterium, and Tetanus
- EU Law and Policy Analysis
- Actinomycetales infections and treatment
- Electrochemical sensors and biosensors
- Nuclear Receptors and Signaling
- Plant Disease Resistance and Genetics
- Erythrocyte Function and Pathophysiology
- Infectious Diseases and Mycology
University of Buenos Aires
2023
Istituto Superiore di Sanità
1997-2017
Città di Castello Hospital
1996
University of Bari Aldo Moro
1991
Assessment of sensitization by allergen-specific IgE testing and skin prick (SPT) are primary tools in routine clinical diagnosis allergies. To perform a correct diagnosis, it is critical that the allergen reagent used contains an adequate amount all relevant components. This study aimed at evaluating commercially available mite extracts for vivo from eight manufacturers.Eight Dermatophagoides pteronyssinus farinae were analysed total protein content Bradford major ELISA. SDS-PAGE,...
Abstract Background The Italian severe/uncontrolled asthma ( SUA ) web‐based registry encompasses demographic, clinical, functional, and inflammatory data; it aims to raise awareness, identifying specific phenotypes promoting optimal care. Methods Four hundred ninety three adult patients from 27 centers (recruited in 2011‐2014) were analyzed. Results Mean age was 53.8 years. more frequently female (60.6%), with allergic (83.1%). About 30% showed late onset of diagnosis/symptoms (>40...
An enzyme-linked immunosorbent assay (ELISA) was adapted for the indirect serological measurement of anti-Echinococcus antibodies in human hydatid disease. Both tube method and microtitration procedure were used successfully. However, test with a purified fluid fraction appears to be choice. ELISA results are comparable those found hemagglutination agar gel methods (double diffusion immunoelectrophoresis), but false positive observed sera patients schistosomiasis or liver cirrhosis. has...
A rapid method for the purification of major 43-kDa allergen Cupressus arizonica pollen, Cup a 1, was developed.The salient feature wash pollen in acidic buffer, followed by an extraction proteins and their chromatography. Immunoblotting, ELISA, lectin binding were tested on both crude extract purified 1. Biochemical analyses performed to assess 1 isoelectric point, its partial amino-acid sequence, glycan composition.Immunochemical analysis confirmed that allergenic reactivity is maintained...
The BSP090 project aims at establishing European Pharmacopoeia Reference Substances in combination with the corresponding ELISA methods for quantification of major allergens allergen products. Two sandwich ELISAs proved suitable Bet v 1, birch pollen allergen, preceding phases BSP090.Two 1-specific systems were compared respect to accuracy and precision a ring trial including 13 laboratories. Model samples containing recombinant rBet 1.0101 as well native extracts measured independently...
Summary Background Cypress pollinosis is an important cause of respiratory allergies. Recently, the Cupressus arizonica major allergen, Cup a1, has been cloned and expressed. The native counterpart this allergen purified characterized by our group. It suggested that sugar moieties play a role in vitro IgE binding on pollen extract. Objective To characterize immunoreactivity recombinant comparison with its counterpart. evaluate carbohydrate IgE‐mediated histamine release from basophils using...
Carbohydrate epitopes are capable of binding human IgE from allergic subjects and these play a role in the cross-reactivity between allergens unrelated sources. A monoclonal antibody (5E6), specific for carbohydrate epitope detectable on components Cupressus arizonica pollen extract, has been produced characterized. To study relationship recognized by subjects. investigate presence such determinant extracts 21 species belonging to 16 taxonomically related families, means antibody....
The in vitro proliferative response to separated immunologically relevant components of Parietaria judaica pollen extract (PjE) was investigated by proliferation assay and limiting dilution analysis, peripheral blood mononuclear cells from Parietaria‐allergic subjects nonallergic controls. In the same subjects, profile antibody PjE fractions also studied immunoblotting evaluate functional significance allergen‐induced T‐cell activation two groups. estimated frequency PjE‐reactive T...
The presence of specific IgE antibodies to invertebrates is common among patients with rhinitis and asthma. Tropomyosin has been described as an invertebrate cross-reactive allergen. We have recently characterized allergenic extract from silverfish (Lepisma saccharina). Since this insect could be a new source tropomyosin in the indoor environment, we thought important clone characterize it.Recombinant was cloned by means immunoblotting tropomyosin-specific monoclonal antibodies, rabbit...
A major allergen of Parietaria officinalis , a species responsible for large number respiratory allergies in Mediterranean areas, has been identified and characterized. This (Pol) was found the fraction which precipitates between 70 100% ammonium sulphate saturation. Pol showed molecular weight 15,000 daltons as determined by SDS‐PAGE HPLC. The pI pH region 4‐6, IEF showing four bands. Two bands were shown CIE, CRIE immuno‐blotting; contaminants or aggregates also revealed latter technique...
Cross‐reactivity between the different components in Parietaria judaica pollen extract has been investigated by polyclonal as well monoclonal antibodies before and after chemical deglycosylation obtained trifluoromethanesulphonic acid (TFMS) treatment of extract. In western blotting a rabbit antiseruni, injecting purified Par j I, was able to recognise many native However, its reactivity restricted, extract, major allergen alone, indicating that cross‐reactivity due sugar moieties. Moreover,...