A5052117451- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Cytokine Signaling Pathways and Interactions
- RNA Research and Splicing
- Protease and Inhibitor Mechanisms
- Immune Cell Function and Interaction
- Animal Genetics and Reproduction
- Mast cells and histamine
- Cellular Mechanics and Interactions
- T-cell and B-cell Immunology
- Cell Adhesion Molecules Research
- RNA and protein synthesis mechanisms
- Electrochemical Analysis and Applications
Universidad Argentina de la Empresa
2019
Consejo Nacional de Investigaciones Científicas y Técnicas
2019
Universidad Maimónides
2013-2015
Weizmann Institute of Science
2002-2004
In recent years, reagentless aptamer biosensors, named aptasensors, have shown significant advancements. Particularly, electrochemical aptasensors could change the field of biosensors in this era, where digitalization seems to be a common goal many fields. Biomedical devices are integrating electronic technologies for detecting pathogens, biomolecules, small molecules, and ions, physical-chemical properties nucleic acid aptamers makes them very interesting these devices. Aptamers can easily...
Abstract Aggregation of type 1 Fcϵ receptors (FcϵRI) on mast cells activates a biochemical cascade that culminates in secretion inflammatory mediators, as well changes cell morphology and adhesion properties. Some the intracellular components involved early coupling events are still unidentified. Here we show two adaptor proteins, downstream tyrosine kinases (Dok)‐1 Dok‐2, FcϵRI rat mucosal‐type RBL‐2H3 line. Dok‐1 is found to be constitutively associated with FcϵRI, even untreated cells,...
Aptamer ligands for myelin basic protein (MBP) were obtained using the systematic evolution of ligand by exponential enrichment (SELEX) method. Two clones isolated from a pool oligonucleotides and tested MBP targeting. Using purified MBP, we demonstrated binding activity aptamers also showed affinity specific length. Moreover, one selected aptamer competitively inhibited an MBP-specific antibody to was found more sensitive than commercial antibody. In addition, ability detect myelin-rich...