A5010387659- Enzyme Structure and Function
- Diet, Metabolism, and Disease
- Protein Structure and Dynamics
- Mass Spectrometry Techniques and Applications
- Amino Acid Enzymes and Metabolism
- X-ray Spectroscopy and Fluorescence Analysis
- Glycosylation and Glycoproteins Research
- Biochemical and Structural Characterization
- DNA Repair Mechanisms
- Biofuel production and bioconversion
- Ion-surface interactions and analysis
- Enzyme Production and Characterization
- Monoclonal and Polyclonal Antibodies Research
- Aldose Reductase and Taurine
- Synthesis and Biological Evaluation
- Biochemical and Molecular Research
- Advanced Electron Microscopy Techniques and Applications
- Fullerene Chemistry and Applications
- Nanoparticles: synthesis and applications
- Photosynthetic Processes and Mechanisms
- Advanced Glycation End Products research
- Kidney Stones and Urolithiasis Treatments
- Oral and gingival health research
- Chemical Reaction Mechanisms
- Multicomponent Synthesis of Heterocycles
Deutsches Elektronen-Synchrotron DESY
2022-2024
Max Delbrück Center
2019-2021
Helmholtz-Zentrum Berlin für Materialien und Energie
2019-2021
University of Oxford
2017-2019
Philipps University of Marburg
2019
University of Eastern Finland
2013-2016
Finland University
2013
Traditionally small-molecule crystallographers have not usually observed or recognized significant radiation damage to their samples during diffraction experiments. However, the increased flux densities provided by third-generation synchrotrons resulted in increasing numbers of observations this phenomenon. The diversity types systems means it is yet possible propose a general mechanism for radiation-induced sample decay, however characterization effects will permit attempts understand and...
A type of protein/peptide pair known as Catcher/Tag spontaneously forms an intermolecular isopeptide bond which can be applied for biomolecular click reactions. Covalent protein conjugation using pairs has turned out to a valuable tool in biotechnology and biomedicines, but it is essential increase the current toolbox orthogonal expand range applications further, example, controlled multiple-fragment ligation. We report here engineering novel ligation, aided by crystal structure minimal CnaB...
Abstract Micro-crystal electron diffraction (MicroED) has shown great potential for structure determination of macromolecular crystals too small X-ray diffraction. However, specimen preparation remains a major bottleneck. Here, we report simple method preparing MicroED specimens, named Preassis, in which excess liquid is removed through an EM grid with the assistance pressure. We show ice thicknesses can be controlled by tuning pressure combination grids appropriate carbon hole sizes....
Radiation damage still remains a major limitation and challenge in macromolecular X-ray crystallography. Some of the high-intensity radiation used for diffraction data collection experiments is absorbed by crystals, generating free radicals. These give rise to even at cryotemperatures (~100 K), which can lead incorrect biological conclusions being drawn from resulting structure, or prevent structure solution entirely. Investigation mitigation strategies effects caused has been extensive over...
In vitro toxicological studies together with atomistic molecular dynamics simulations show that occupational co-exposure C60 fullerene may strengthen the health effects of organic industrial chemicals. The chemicals studied are acetophenone, benzaldehyde, benzyl alcohol, m-cresol, and toluene which can be used as reagents or solvents in processes. Potential scenarios include a dust chemical vapor, solution aerosolized workplace air. Unfiltered filtered mixtures represent different where...
Xylose isomerase (XI) is an industrially important metalloprotein studied for decades. Its reaction mechanism has been postulated to involve movement of the catalytic metal cofactor several different conformations. Here, a dose-dependent approach was used investigate radiation damage effects on XI and their potential influence interpreted from X-ray derived structures. Radiation still one major challenges diffraction experiments causes both global site-specific damage. In this study,...
Agrobacterium tumefaciens (At) strain C58 contains an oxidative enzyme pathway that can function on both d-glucuronic and d-galacturonic acid. The corresponding gene coding for At keto-deoxy-d-galactarate (KDG) dehydratase is located in the same cluster as those uronate dehydrogenase (At Udh) galactarolactone cycloisomerase Gci) which we have previously characterized. Here, present kinetic characterization crystal structure of KDG dehydratase, catalyzes next step, decarboxylating hydrolyase...
Aldose–aldose oxidoreductase (Cc AAOR) is a recently characterized enzyme from the bacterial strain Caulobacter crescentus CB15 belonging to glucose-fructose oxidoreductase/inositol dehydrogenase/rhizopine catabolism protein (Gfo/Idh/MocA) family. Cc AAOR catalyses oxidation and reduction of panel aldose monosaccharides using tightly bound NADP(H) cofactor that regenerated in catalytic cycle. Furthermore, can also oxidize 1,4-linked oligosaccharides. In present study, we novel crystal...
Abstract Micro-crystal electron diffraction (MicroED) has shown great potential for structure determination of macromolecular crystals too small X-ray diffraction. However, specimen preparation remains a major bottleneck. Here, we report simple method preparing MicroED specimens, named Preassis, in which excess liquid is removed through an EM grid with the assistance pressure. We show ice thicknesses can be controlled by tuning pressure combination grids appropriate hole sizes. Importantly,...
Abstract magnified image Imidazole‐4(5)‐carboxaldehyde and 4(5)‐cyanoimidazole were N‐benzylated N‐methylated using benzyl chloride methyl iodide on zinc oxide (ZnO), alumina, KF/alumina under basic conditions without solvent. Triethylamine (Et 3 N) or potassium carbonate was added as base in the reactions ZnO alumina. also benzylated silica carbon nanotubes. The effect of bases solids product distribution 1,4‐ 1,5‐substituted compounds investigated. In some cases, ratios different for...
Abstract A type of protein/peptide pair known as Catcher/Tag spontaneously forms an intermolecular isopeptide bond which can be applied for biomolecular click reactions. Covalent protein conjugation using pairs has turned out to a valuable tool in biotechnology and biomedicines, but it is essential increase the current toolbox orthogonal expand range applications further, example, controlled multiple‐fragment ligation. We report here engineering novel ligation, aided by crystal structure...
d-Galacturonic acid is the main component of pectin. It could be used to produce affordable renewable fuels, chemicals and materials through biotechnical conversion. Keto-deoxy-d-galactarate (KDG) dehydratase an enzyme in oxidative pathway d-galacturonic Agrobacterium tumefaciens (At). converts 3-deoxy-2-keto-l-threo-hexarate α-ketoglutaric semialdehyde. At KDG was crystallized by hanging-drop vapour-diffusion method. The crystals belonged monoclinic space group C2, with unit-cell parameters...
We report the use of streaming data interfaces to perform fully online processing for serial crystallography experiments, without storing intermediate on disk. The system produces Bragg reflection intensity measurements suitable scaling and merging, with a latency less than 1 s per frame. Our uses CrystFEL software in combination ASAP::O framework. In series user experiments at PETRA III, frames from 16 megapixel Dectris EIGER2 X detector were searched peaks, indexed integrated maximum...
Abstract Protein/peptide pairs, called Catcher/Tag are applied for biological isopeptide-bond mediated Click-reactions. Covalent protein ligation using pairs has turned out to be a valuable tool in biotechnology and biomedicines. It is essential increase the current toolbox of expand range applications further, e.g., multiple-fragment that requires several orthogonal ligases. We report here engineering novel ligation, aided by new crystal structure minimal CnaB domain from Lactobacillus...
The author wishes to make the following corrections this paper [1]:[...]
Abstract Micro-crystal electron diffraction (MicroED) has shown great potential for structure determination of macromolecular crystals too small X-ray diffraction. However, specimen preparation remains a major bottleneck. Here, we report simple method preparing MicroED specimens, named Preassis, in which excess liquid is removed through an EM grid with the assistance pressure. We show ice thicknesses can be controlled by tuning pressure combination grids appropriate hole sizes. Importantly,...