A5092206581- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Big Data and Business Intelligence
- Scientific Computing and Data Management
- Research Data Management Practices
Uppsala University
2023-2025
University of Florence
2023
Reporter systems are widely used to study biomolecular interactions and processes in vivo, representing one of the basic tools characterize synthetic regulatory circuits. Here, we developed a method that enables monitoring RNA–protein through reporter system bacteria with high temporal resolution. For this, Real-Time Protein Expression Assay (RT-PEA) technology for real-time fluorescent protein, while having growing on solid media. Experimental results were analyzed by fitting three-variable...
The kinetics of the interaction between Musashi-1 (MSI1) and RNA have been characterized using surface plasmon resonance biosensor analysis. Truncated variants human MSI1 encompassing two homologous recognition motifs (RRM1 RRM2) in tandem (aa 1–200), RRMs isolation 1–103 aa 104–200, respectively) were produced. proteins injected over sensor surfaces with immobilized RNA, varying sequence length, one or RRM binding motifs. interactions individual all well described by a 1:1 model. variant...
Background: Data management is fast becoming an essential part of scientific practice, driven by open science and FAIR (findable, accessible, interoperable, reusable) data sharing requirements. Whilst plans (DMPs) are clear to experts stewards, understandings their purpose creation often obscure the producers data, which in academic environments PhD students. Methods: Within RNAct EU Horizon 2020 ITN project, we engaged 10 early-stage researchers (ESRs) a training project aimed at...