A5068483012- Signaling Pathways in Disease
- Biochemical and Molecular Research
- Gut microbiota and health
- Protein Kinase Regulation and GTPase Signaling
- Plant Molecular Biology Research
- Curcumin's Biomedical Applications
- Retinal Development and Disorders
- Metabolism, Diabetes, and Cancer
- Plant Parasitism and Resistance
- Toxin Mechanisms and Immunotoxins
- Plant Genetic and Mutation Studies
- Quinazolinone synthesis and applications
- PI3K/AKT/mTOR signaling in cancer
- Bioactive Compounds and Antitumor Agents
- Cancer Mechanisms and Therapy
- Cellular transport and secretion
- RNA regulation and disease
- Beetle Biology and Toxicology Studies
- Enzyme Structure and Function
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Ocular Oncology and Treatments
- Medicinal Plants and Neuroprotection
- Pancreatic function and diabetes
- Bone Tumor Diagnosis and Treatments
- Nanoparticles: synthesis and applications
University of North Carolina at Chapel Hill
2020-2025
University of North Carolina Health Care
2024
Nanyang Technological University
2014-2016
Heterotrimeric guanine nucleotide–binding proteins (G proteins) that function as molecular switches for cellular growth and metabolism are activated by GTP inactivated hydrolysis. In uveal melanoma, a conserved glutamine residue critical hydrolysis in the G protein α subunit is often mutated Gα q or 11 to either leucine proline. contrast, other mutations subtypes rare. To uncover mechanism of genetic selection functional role this residue, we analyzed all possible substitutions multiple...
Heterotrimeric G proteins (Gα, Gβ and Gγ) act downstream of G-protein-coupled receptors (GPCRs) to mediate signaling pathways that regulate various physiological processes human disease conditions. While Gαi its yeast homolog Gpa1 were previously postulated function as intracellular pH sensors, the pH-sensing capabilities underlying mechanism remain be established. Our research shows variations in significantly affect structure stability Gαi-GDP. Specifically, at lower end range, protein...
The nuclear immunophilin FKBP25 interacts with chromatin-related proteins and transcription factors is suggested to interact nucleic acids. Currently the structural basis of acid binding by unknown. Here we determined magnetic resonance (NMR) solution structure full-length human studied its interaction DNA. revealed that N-terminal helix-loop-helix (HLH) domain C-terminal FK506-binding (FKBD) each other both domains are involved in DNA binding. HLH forms major-groove interactions basic FKBD...
Human FKBP25 (hFKBP25) is a nuclear immunophilin and interacts with several proteins, hence involving in many events. Similar to other FKBPs, FK506 binding domain (FKBD) of hFKBP25 also binds immunosuppressive drugs such as rapamycin FK506, albeit lower affinity for the latter. The molecular basis underlying this difference could not be addressed due lack crystal structure hFKBD25 complex FK506. Here, we report determined at 1.8 Å resolution its comparison hFKBD25-rapamycin complex, bringing...
is the third most common cause of nosocomial infections. Linezolid (LNZ) a reserve antibiotic recommended for infections caused by vancomycin resistant
Heterotrimeric G proteins (Gα, Gβ and Gγ) act downstream of G-protein-coupled receptors (GPCRs) to mediate signaling pathways that regulate various physiological processes human disease conditions. Previously, Gαi its yeast homolog Gpa1 have been reported function as intracellular pH sensors, yet the sensing capabilities underlying mechanism remain be established. Herein, we identify a network within Gαi, evaluate consequences modulation on structure stability G-protein. We find changes over...