Amphiphilicity is the most critical parameter in self-assembly of surfactant-like peptides (SLPs), regulating way by which hydrophobic attraction holds together. Its effects go beyond supramolecular assembly and may also trigger different cell responses bioactive peptide-based nanostructures. Herein, we investigate cellular nanostructures based on isomeric SLPs composed arginine (R) phenylalanine (F). Two amphipathic designs were studied: a diblock construct F4R4 its bolaamphiphile analog...

A series of tripodal (three-arm) lysine-based peptides were designed and synthesized their self-assembly properties in aqueous solution antimicrobial activity investigated. We compare the behaviors homochiral (KKY)3K a homologue containing bulky aromatic fluorenylmethoxycarbonyl (Fmoc) group Fmoc-(KKY)3K, heterochiral analogues k (d-Lys), (kkY)3K Fmoc-(kkY)3K. The molecular conformation solutions probed using various spectroscopic techniques, along with small-angle X-ray scattering (SAXS)...

ABSTRACT Lipoic acid is a biocompatible compound with antioxidant activity that of considerable interest in cosmetic formulations, and the disulfide group N‐terminal ring confers redox activity. Here, we study self‐assembly aspects bioactivity lipopeptide (peptide amphiphile) comprising KTTKS collagen‐stimulating pentapeptide sequence conjugated to an lipoic chain, lipoyl‐KTTKS. Using SAXS, SANS cryo‐TEM, lipoyl‐KTTKS found form population curly fibrils (wormlike micelles) above critical...

Toll-like receptor (TLR) agonists are of interest in immunotherapy and cancer vaccines. The most common TLR2 based on Pam2Cys or Pam3Cys. In the former, two palmitoyl (Pam) fatty acids linked to a glycerylcysteine motif by ester linkages. Pam3Cys is analogous but contains an extra Pam group α-amine. Here, we compare self-assembly aqueous solution parent Pam2CysOH amino acid conjugates that Pam2CysSK4 Pam3CysSK4 which potent bearing CysSK4 peptide sequence. All four exhibit critical...

The self‐assembly in aqueous solution of three Fmoc‐amino acids with hydrophobic (aliphatic or aromatic, alanine phenylalanine) hydrophilic cationic residues (arginine) is compared. critical aggregation concentrations were obtained using intrinsic fluorescence probe measurements, and conformation was probed circular dichroism spectroscopy. Self‐assembled nanostructures imaged cryo‐transmission electron microscopy small‐angle X‐ray scattering (SAXS). Fmoc‐Ala found to form remarkable...

The interaction of the surfactant-like peptide (SLP) R

Cell-penetrating peptides (CPPs) are a topical subject potentially exploitable for creating nanotherapeutics the delivery of bioactive loads. These compounds often classified into three major categories according to their physicochemical characteristics: cationic, amphiphilic, and hydrophobic. Among them, group hydrophobic CPPs has received increasing attention in recent years due toxicity concerns posed by highly cationic CPPs. The hexapeptide PFVYLI (P, proline; F, phenylalanine; V,...

The emergence of drug-resistant pathogenic microorganisms has become a public health concern, with demand for strategies to suppress their proliferation in healthcare facilities. present study investigates the physicochemical and antimicrobial properties carbon dots (CD-MR) derived from methyl red azo dye. morphological structural analyses reveal that such significant fraction graphitic nitrogen structures, providing wide emission range. Based on low cytotoxicity against mammalian cells...

A heptamer containing the longest noncationic amino acid sequence of<italic>Penetratin</italic>is shown to form peptiplexes with DNA. The structure is characterized from molecular nanoscopic scale and are assist intracell delivery of

One of the most remarkable examples cell-penetrating peptides (CPPs) is Penetratin, a 16-mer fragment derived from Drosophila Antennapedia homeobox. Understanding structure Penetratin/DNA complexes key factor for successful design new vectors gene delivery and may assist in optimizing molecular carriers based on CPPs. Herein, we present comprehensive study nanoscale noncovalent formed between Penetratin DNA. The strong cationic nature peptide makes it very efficient agent condensing DNA...

The conformation and self-assembly of four lipopeptides, peptide amphiphiles comprising peptides conjugated to lipid chains, in aqueous solution have been examined. sequence all lipopeptides contains the integrin cell adhesion RGDS motif, cytocompatibility is also analyzed. Lipopeptides either tetradecyl (C14, myristyl) or hexadecyl (C16, palmitoyl) chains WGGRGDS GGGRGDS, that is, with a tryptophan-containing WGG triglycine GGG tripeptide spacer between bioactive motif alkyl chain. All...

Peptide-based hydrogels have attracted much attention due to their extraordinary applications in biomedicine and offer an excellent mimic for the 3D microenvironment of extracellular matrix. These hydrated matrices comprise fibrous networks held together by a delicate balance intermolecular forces. Here, we investigate hydrogelation behavior designed decapeptide containing tetraleucine self-assembling backbone fibronectin-related tripeptides near both ends strand. We observed that this...

Three model arginine-rich tripeptides RXR (X = W, F or non-natural residue 2-napthylalanine) were investigated as antimicrobial agents, with a specific focus to target Pseudomonas aeruginosa through membrane lysis. Activity against biofilms was related binding of the second messenger molecule, nucleotide bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP). Strong selective activity P. in planktonic form observed for RFR and RWR.

Despite numerous efforts over the last three decades, nucleic acid-based therapeutics still lack delivery platforms in clinical stage. Cell-penetrating peptides (CPPs) may offer solutions as potential vectors. We have previously shown that designing a "kinked" structure peptide backbone resulted CPP with efficient vitro transfection properties. Further optimization of charge distribution C-terminal part led to potent vivo activity resultant NickFect55 (NF55). Currently, impact linker amino...

Glutamic acid–rich peptides are crucial to a variety of biological processes, including glutamatergic neurotransmission and immunological defense. acid sequences often exhibit unusual organization into β 2 ‐type sheets, where bifurcated H bonds formed between glutamic side chains NH in amide on adjacent ‐ strands play paramount role for stabilizing the molecular assembly. Herein, we investigate self‐assembly supramolecular structure simplified models consisting alternating acid/phenylalanine...

Short and ultra-short peptides have recently emerged as suitable building blocks for the fabrication of self-assembled innovative materials. Peptide aggregation is strictly related to amino acids composing sequence their capability establish intermolecular interactions. Additional structural functional properties can also be achieved by peptide derivatization (e.g. with polymeric moieties, alkyl chains or other organic molecules). For instance, amphiphiles (PAs), containing one more tails on...

Bradykinin (BK) is a peptide hormone that plays crucial role in blood pressure control, regulates inflammation the human body, and has recently been implicated pathophysiology of COVID-19. In this study, we report strategy for fabricating highly ordered 1D nanostructures BK using DNA fragments as template self-assembly. We have combined synchrotron small-angle X-ray scattering high-resolution microscopy to provide insights into nanoscale structure BK-DNA complexes, unveiling formation...

Cell penetrating peptides (CPPs) have emerged as promising materials for the fabrication of synthetic nanovectors endowed with potential improving future landscape gene therapy.

There has been considerable interest in peptides which the Fmoc (9-fluorenylmethoxycarbonyl) protecting group is retained at N-terminus, since this bulky aromatic can drive self-assembly, and Fmoc-peptides are biocompatible have applications cell culture biomaterials. Recently, analogues of new amino acids with 2,7-disulfo-9-fluorenylmethoxycarbonyl (Smoc) groups developed for water-based peptide synthesis. Here, we report on self-assembly biocompatibility Smoc-Ala, Smoc-Phe Smoc-Arg as...

Peptide-polymer systems hold strong potential for applications in nanotherapeutics. Desmopressin, a synthetic analogue of the antidiuretic hormone arginine vasopressin, may serve as valuable case study this context since it is first-line treatment disorders affecting water homeostasis, including diabetes insipidus. It also has an established use hemostatic agent von Willebrand disease, and recently, its repurposing been suggested neoadjuvant certain types cancer. Despite well-documented...