A5056159531- Galectins and Cancer Biology
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Signaling Pathways in Disease
- Protein Tyrosine Phosphatases
- Immunotherapy and Immune Responses
- Tissue Engineering and Regenerative Medicine
- Toxin Mechanisms and Immunotoxins
- Cell Adhesion Molecules Research
- Immune Response and Inflammation
- Blood groups and transfusion
- Educational Research and Science Teaching
- Diabetes and associated disorders
- Erythrocyte Function and Pathophysiology
- Cardiomyopathy and Myosin Studies
- Medical Imaging and Pathology Studies
- Muscle Physiology and Disorders
- Veterinary medicine and infectious diseases
- Ethics and bioethics in healthcare
- Health and Medical Education
- Cancer Cells and Metastasis
- High Entropy Alloys Studies
Brigham and Women's Hospital
2022-2024
Harvard University
2022-2024
Brigham Young University
2019-2023
Abstract The RGD (Arg-Gly-Asp)-binding integrins αvβ6 and αvβ8 are clinically validated cancer fibrosis targets of considerable therapeutic importance. Compounds that can discriminate between homologous other integrins, stabilize specific conformational states, have high thermal stability could utility. Existing small molecule antibody inhibitors do not all these properties, hence new approaches needed. Here we describe a generalized method for computationally designing RGD-containing...
Although immune tolerance evolved to reduce reactivity with self, it creates a gap in the adaptive response against microbes that decorate themselves self-like antigens. This is particularly apparent carbohydrate-based blood group antigens, wherein can envelope structures similar human cells. In this study, we demonstrate innate lectin, galectin-4 (Gal-4), exhibits strain-specific binding and killing behavior towards display group-like Examination of preferences using combination microarrays...
Micropatterned suspension culture creates consistently sized and shaped cell aggregates but has not produced organotypic structures from stable cells, thus restricting its use in accurate disease modeling. Here, we show that structure is achieved hybrid via supplementation of soluble extracellular matrix (ECM). We created a viable lung organoid epithelial, endothelial, fibroblast human lines culture. demonstrate the importance ECM patterning with emergence lumen-like airspace showing...
Antibodies to red blood cell (RBC) alloantigens can increase morbidity and mortality in transfusion recipients. However, alloimmunization rates vary dramatically, with some patients never generating alloantibodies following transfusion, while others not only become alloimmunized, but may be prone additional subsequent transfusion. Previous studies suggest that CD4 T responses drive alloantibody formation recognize the same alloantigen engaged by B cells. as RBCs express numerous antigens,...
Microbial glycan microarrays (MGMs) populated with purified microbial glycans have been used to define the specificity of host immune factors toward microbes in a high throughput manner. However, limitation such arrays is that presentation may not fully recapitulate natural exists on microbes. This raises possibility interactions observed array, while often helpful predicting actual intact microbes, always accurately ascertain overall affinity factor for given microbe. Using galectin-8...
Limb-girdle muscular dystrophy type 2B (LGMD2B) is caused by mutations in the dysferlin gene, resulting non-functional dysferlin, a key protein found muscle membrane. Treatment options available for patients are chiefly palliative nature and focus on maintaining ambulation. Our hypothesis that galectin-1 (Gal-1), soluble carbohydrate binding protein, increases membrane repair capacity myogenic potential of dysferlin-deficient cells fibers. To test this hypothesis, we used recombinant human...
Abstract The RGD (Arg-Gly-Asp)-binding integrins αvβ6 and αvβ8 are clinically validated cancer fibrosis targets of considerable therapeutic importance. Compounds that can discriminate between the two closely related integrin proteins other integrins, stabilize specific conformational states, have sufficient stability enabling tissue restricted administration could utility. Existing small molecules antibody inhibitors do not all these properties, hence there is a need for new approaches. Here...
Two of the main pathologies characterizing dysferlinopathies are disrupted muscle membrane repair and chronic inflammation, which lead to symptoms weakness wasting. Here, we used recombinant human Galectin-1 (rHsGal-1) as a therapeutic for LGMD2B mouse models. Various redox multimerization states Gal-1 show that rHsGal-1 is most effective form in both increasing decreasing due its monomer-dimer equilibrium. Dose-response testing shows an 25-fold safety profile between 0.54 13.5 mg/kg Bla/J...
Los ensayos clínicos representan el penúltimo escalón de la jerarquía evidencia científica y son esenciales en desarrollo nuevas terapias. Por ello, rigurosidad diseño las consideraciones éticas deben estar implícitos todo proceso construcción evaluación conocimiento. Durante investigación científica, componente ético además ser crucial también cumple con un rol prioritario sobre cualquier tipo intereses. Al momento diseñar estudio su posterior evaluación, comité responsable cumplir ética...
Abstract Micropatterned suspension culture creates consistently sized and shaped cell aggregates but has not produced organotypic structures from stable cells, thus restricting its use in accurate disease modeling. Here, we show that structure is achieved hybrid via supplementation of soluble extracellular matrix (ECM). We created a viable lung organoid epithelial, endothelial, fibroblast human lines culture. demonstrate the importance ECM patterning with emergence lumen-like airspace...