A5030020707- Multiple Sclerosis Research Studies
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Viral gastroenteritis research and epidemiology
- Galectins and Cancer Biology
- SARS-CoV-2 and COVID-19 Research
- Glycosylation and Glycoproteins Research
- Peripheral Neuropathies and Disorders
- Hepatitis Viruses Studies and Epidemiology
- Viral Infections and Immunology Research
- Immunotherapy and Immune Responses
- Toxin Mechanisms and Immunotoxins
- Amyotrophic Lateral Sclerosis Research
- Circular RNAs in diseases
- RNA regulation and disease
- Immune Cell Function and Interaction
- Rheumatoid Arthritis Research and Therapies
- T-cell and B-cell Immunology
- RNA Research and Splicing
- Blood groups and transfusion
- Skin and Cellular Biology Research
- RNA Interference and Gene Delivery
- Diabetes and associated disorders
- Xenotransplantation and immune response
- Tryptophan and brain disorders
Brigham and Women's Hospital
2016-2024
Harvard University
2016-2024
Neurology, Inc
2021-2022
Tel Aviv University
2018-2022
Massachusetts General Hospital
2016-2022
Novartis (Switzerland)
2022
Emory University
2021
Center for Neuro-Oncology
2021
Christian Medical College & Hospital
2010-2020
Amrita Institute of Medical Sciences and Research Centre
2019-2020
Neurodegeneration and astrocytic activation are pathologic hallmarks of progressive multiple sclerosis (MS) can be quantified by serum neurofilament light chain (sNfL) glial fibrillary acidic protein (sGFAP). We investigated sNfL sGFAP as tools for stratifying patients with MS based on progression disease activity status.We leveraged our Comprehensive Longitudinal Investigation at the Brigham Women's Hospital (CLIMB) natural history study, which includes clinical, MRI data samples collected...
To assess the value of annual serum neurofilament light (NfL) measures in predicting 10-year clinical and MRI outcomes multiple sclerosis (MS).We identified patients our center's Comprehensive Longitudinal Investigations MS at Brigham Women's Hospital (CLIMB) study enrolled within 5 years disease onset, with blood samples up to 10 (n = 122). Serum NfL was measured using a single molecule array (SIMOA) assay. An automated pipeline quantified brain T2 hyperintense lesion volume (T2LV)...
Background. Probiotics have a possible role in the treatment of pediatric acute gastroenteritis. We report effect probiotic Lactobacillus rhamnosus GG (LGG) on intestinal function, immune response, and clinical outcomes Indian children with cryptosporidial or rotavirus diarrhea. Methods. Children gastroenteritis aged 6 months to 5 years, testing positive for either Cryptosporidium species stool (coinfections were excluded), randomized LGG (ATCC 53103) placebo, once daily 4 weeks. Baseline...
Amyotrophic lateral sclerosis (ALS) is a debilitating neurologic disorder with poor survival rates and no clear biomarkers for disease diagnosis prognosis.We compared serum microRNA (miRNA) expression from patients ALS healthy controls multiple Alzheimer disease. We also correlated miRNA in cross-sectional longitudinal cohorts of clinical parameters.We identified 7 miRNAs (miR-192-5p, miR-192-3p, miR-1, miR-133a-3p, miR-133b, miR-144-5p, miR-19a-3p) that were upregulated 6 (miR-320c,...
To identify circulating microRNAs (miRNAs) linked to disease stage and disability in multiple sclerosis (MS).Sera from 296 participants including patients with MS, other neurologic diseases (Alzheimer amyotrophic lateral sclerosis), inflammatory (rheumatoid arthritis asthma) healthy controls (HCs) were tested. miRNA profiles determined using LNA (locked nucleic acid)-based quantitative PCR. Patients MS categorized according disability. In the discovery phase, 652 miRNAs measured sera 26 20...
Bioprosthetic heart valves (BHVs) are commonly used to replace severely diseased but their susceptibility structural valve degeneration (SVD) limits use in young patients. We hypothesized that antibodies against immunogenic glycans present on BHVs, particularly the xenoantigens galactose-α1,3-galactose (αGal) and N-glycolylneuraminic acid (Neu5Gc), could mediate deterioration through calcification. established a large longitudinal prospective international cohort of patients (n = 1668, 34 ±...
Abstract The multifaceted nature of multiple sclerosis requires quantitative biomarkers that can provide insights related to diverse physiological pathways. To this end, proteomic analysis deeply-phenotyped serum samples, biological pathway modeling, and network were performed elucidate inflammatory neurodegenerative processes, identifying sensitive disease activity. Here, we evaluated the concentrations > 1400 proteins in 630 samples from three cohorts for association with clinical...
<h3>Objective</h3> To identify circulating microRNAs (miRNAs) linked to disease, disease stage, and disability in MS across cohorts. <h3>Methods</h3> Samples were obtained from the Comprehensive Longitudinal Investigation of Multiple Sclerosis (CLIMB, Boston, MA), EPIC (San Francisco, CA), AMIR (Beirut, Lebanon) as part SUMMIT consortium, Stockholm Prospective Assessment (Stockholm, Sweden) Serum miRNA expression was measured using locked nucleic acid–based quantitative PCR. Four groups...
<h3>Importance</h3> MicroRNAs (miRNAs) are promising multiple sclerosis (MS) biomarkers. Establishing the association between miRNAs and magnetic resonance imaging (MRI) measures of disease severity will help define their significance potential impact. <h3>Objective</h3> To correlate circulating in serum patients with MS to brain spinal MRI. <h3>Design, Setting, Participants</h3> A cross-sectional study comparing miRNA samples MRI metrics was conducted at a tertiary referral center. Two...
Abstract Objective Multiple sclerosis (MS) is an autoimmune demyelinating disorder, which characterized by relapses and remissions. Serum neurofilament light chain (sNfL) emerging biomarker of disease activity but its clinical use still limited. In this study, we aim to characterize the temporal association between sNfL new gadolinium‐enhancing (Gd+) lesions. Methods Annual levels were measured with a single‐molecule array (SIMOA) assay in 94 patients MS enrolled Comprehensive Longitudinal...
Although immune tolerance evolved to reduce reactivity with self, it creates a gap in the adaptive response against microbes that decorate themselves self-like antigens. This is particularly apparent carbohydrate-based blood group antigens, wherein can envelope structures similar human cells. In this study, we demonstrate innate lectin, galectin-4 (Gal-4), exhibits strain-specific binding and killing behavior towards display group-like Examination of preferences using combination microarrays...
Abstract Reverse transcription‐real‐time polymerase chain reaction (RT‐qPCR) for the VP6 gene was used to study group A rotavirus shedding in children with symptomatic and asymptomatic infection. Sequential stool samples (n = 345) from 10 associated diarrhea five 161) infection were collected over a period of 2 months. RT‐qPCR assay on using plasmid standard demonstrated high reproducibility, an inter‐assay coefficient variation (CV) 1.40–2.97% intra‐assay CV 0.03–3.03%. The median duration...
A disequilibrium between immunosuppressive Tregs and inflammatory IL-17-producing Th17 cells is a hallmark of autoimmune diseases, including multiple sclerosis (MS). However, the molecular mechanisms underlying Treg imbalance in CNS autoimmunity remain largely unclear. Identifying factors that drive this high clinical interest. Here, we report major disease-promoting role for microRNA-92a (miR-92a) autoimmunity. miR-92a was elevated experimental encephalomyelitis (EAE), its loss attenuated...
Several factors influence the clinical course of autoimmune inflammatory diseases such as MS and bowel disease. Only recently, complex interaction between gut microbiome, dietary factors, metabolism has started to be appreciated with regard its potential modulate acute chronic inflammation. One molecular sensors that mediates effects these environmental signals on immune response is aryl hydrocarbon receptor (AHR), a ligand-activated transcription factor key functions in cells.In this study,...
T cells are present in early stages of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and play a major role disease outcome long-lasting immunity. Nasal administration fully human anti-CD3 monoclonal antibody (Foralumab) reduced lung inflammation as well serum IL-6 C-reactive protein moderate cases COVID-19. Using proteomics RNA-sequencing, we investigated immune changes patients treated with nasal Foralumab. In randomized trial, mild to COVID-19 outpatients...
Rotavirus gastroenteritis results in significant morbidity and mortality Indian children. Although there are numerous studies on rotavirus diarrhea, few reports antigenemia extraintestinal presentations these populations.Following screening for antigen of stool samples from children with without acute a commercial enzyme immunoassay (EIA), total 199 serum sample pairs were identified additional testing. All EIA-positive genotyped, viral load estimated by real-time reverse-transcriptase...
This study was undertaken to compare the immunogenicity of a three dose and five schedule an oral live-attenuated human rotavirus vaccine, Rotarix® in south Indian infants. Healthy infants (N = 90), six seven weeks age were enrolled receive doses (n 45) or Rotarix vaccine along with other scheduled vaccines, each separated by four week interval. Blood samples taken before vaccination one month post-dose group group; all tested for anti-rotavirus IgA antibody sandwich enzyme immunoassay. At...
Accurate diagnosis of acute severe respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is critical for appropriate management patients with this disease. We examined the possible complementary role laboratory-developed class-specific clinical serology in assessing SARS-CoV-2 hospitalized patients.