A5051256177- Legionella and Acanthamoeba research
- Enzyme Structure and Function
- Bacterial Genetics and Biotechnology
- Calcium signaling and nucleotide metabolism
- Endoplasmic Reticulum Stress and Disease
- Biochemical and Molecular Research
- Autophagy in Disease and Therapy
- Cancer Research and Treatments
- Toxin Mechanisms and Immunotoxins
- Genomics and Phylogenetic Studies
- PARP inhibition in cancer therapy
- Antifungal resistance and susceptibility
- Heat shock proteins research
- Nematode management and characterization studies
- Ubiquitin and proteasome pathways
- Antibiotic Resistance in Bacteria
- RNA and protein synthesis mechanisms
- Amino Acid Enzymes and Metabolism
- Blood disorders and treatments
- Vibrio bacteria research studies
- Protein Structure and Dynamics
- Bacteriophages and microbial interactions
- Microbial Community Ecology and Physiology
- Neonatal Health and Biochemistry
- Connexins and lens biology
Warsaw University of Life Sciences
2017-2024
Divergent protein kinase SidJ is a produced by Legionella pneumophila that orchestrates this intracellular pathogen's establishment within the host cell. prevents lysosome fusion with vacuole, which bacterium resides and replicates. also modulates toxicity of SidE family ubiquitin ligases catalyze phosphoribosyl-linked ubiquitination. Black et al. discovered activated calmodulin. Furthermore, although has pseudokinase fold, it does not phosphorylate proteins but polyglutamylates them...
The study aimed to comprehensively investigate the structural dynamics of a soil microbial community within 100-year agricultural field experiment. experimental design encompasses three distinct management practices, namely monoculture, five–year rotation, and random each with or without incorporation legumes manure. Soil communities were determined by Next-Generation Sequencing (Illumina MiSeq analysis) both V3 V4 hypervariable regions 16S rRNA gene for bacteria Archaea, as well ITS1 fungi....
ADP-ribosyltransferases (ARTs) are a widespread superfamily of enzymes frequently employed in pathogenic strategies bacteria. Legionella pneumophila, the causative agent severe form pneumonia known as Legionnaire's disease, has acquired over 330 translocated effectors that showcase remarkable biochemical and structural diversity. However, ART influence L. pneumophila have not been well defined. Here, we took bioinformatic approach to search effector repertoire for additional divergent...
The presence of many completely uncharacterized proteins, even in well-studied organisms such as humans, seriously hampers full understanding the functioning living cells. ADP-ribosylation is a common post-translational modification proteins; also nucleic acids and small molecules can be modified by covalent attachment ADP-ribose. This modification, important cellular signalling infection processes, usually executed enzymes from large superfamily ADP-ribosyltransferases (ARTs). Here, using...
The pathogenic Legionella bacteria are notorious for delivering numerous effector proteins into the host cell with aim of disturbing and hijacking cellular processes their benefit. Despite intensive studies, many effectors remain uncharacterized. Motivated by richness repertoires oftentimes atypical biochemistry, also several known kinases pseudokinases discovered recently, we undertook an in silico survey exploration pan-kinome genus, i.e., union kinomes individual species. In this study,...
Many bacterial effector proteins that are delivered to host cells during infection enzymes targeting cell signalling. Recently, Legionella pneumophila Lpg1137 was experimentally characterised as a serine protease cleaves human syntaxin 17. We present strong bioinformatic evidence is homologue of mitochondrial carrier and not related known proteases. also discuss how this finding can be reconciled with the apparently contradictory experimental results.
Abstract Objective The superfamily of protein kinases features a common Protein Kinase-like (PKL) three-dimensional fold. Proteins with PKL structure can also possess enzymatic activities other than phosphorylation, such as AMPylation or glutamylation. proteins play vital role in the world living organisms, contributing to survival pathogenic bacteria inside host cells, well being involved carcinogenesis and neurological diseases humans. is constantly growing. Therefore, it crucial gather...
Abstract Background ADP-ribosyltransferases (ARTs) are a superfamily of enzymes implicated in various cellular processes, including pathogenic mechanisms. The Legionella genus, known for causing Legionnaires’ disease, possesses diverse ART-like effectors. This study explores the proteomes 41 species to bioinformatically identify and characterise novel families, providing insights into their potential roles pathogenesis host interactions. Methods We conducted comprehensive bioinformatic...
Abstract The presence of many completely uncharacterized proteins, even in well-studied organisms such as humans, seriously hampers full understanding the functioning living cells. ADP-ribosylation is a common post-translational modification proteins; also nucleic acids and small molecules can be modified by covalent attachment ADP-ribose. This modification, important cellular signalling infection processes, usually executed enzymes from large superfamily ADP-ribosyltransferases (ARTs) Here,...
Abstract Objective The superfamily of protein kinases features a common Protein Kinase-like (PKL) three-dimensional fold. Proteins with PKL structure can also possess enzymatic activities other than phosphorylation, such as AMPylation or glutamylation. proteins play vital role in the world living organisms, contributing to survival pathogenic bacteria inside host cells, well being involved carcinogenesis and neurological diseases humans. is constantly growing. Therefore, it crucial gather...
Abstract ADP-ribosyltransferases (ARTs) are a widespread superfamily of enzymes frequently employed in pathogenic strategies bacteria. Legionella pneumophila , the causative agent Legionnaire’s disease, has acquired over 330 translocated effectors that showcase remarkable biochemical and structural diversity. Here we took bioinformatic approach to search effector repertoire for additional divergent members ART identified an domain Lpg0181. We show L . Lpg0181 targets specific class 120-kDa...
Abstract The pathogenic Legionella bacteria are notorious for delivering numerous effector proteins into the host cell with aim of disturbing and hijacking cellular processes their benefit. Despite intensive studies, many effectors remain uncharacterized. Motivated by richness repertoires oftentimes atypical biochemistry, also several known kinases pseudokinases, we undertook an in silico survey exploration pan-kinome genus, i.e., union kinomes individual species. In this study, discovered...