A5043463659- Legume Nitrogen Fixing Symbiosis
- Plant Molecular Biology Research
- Plant Physiology and Cultivation Studies
- Agricultural pest management studies
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- Genetic and Environmental Crop Studies
- Microbial Metabolites in Food Biotechnology
- Plant tissue culture and regeneration
- Light effects on plants
- Plant Pathogenic Bacteria Studies
- Agricultural Systems and Practices
- Nematode management and characterization studies
- Plant Taxonomy and Phylogenetics
- Botany and Plant Ecology Studies
- Flowering Plant Growth and Cultivation
- Chromosomal and Genetic Variations
- Horticultural and Viticultural Research
- Agroforestry and silvopastoral systems
- Ruminant Nutrition and Digestive Physiology
- Soil Carbon and Nitrogen Dynamics
- Agronomic Practices and Intercropping Systems
- Genetic diversity and population structure
- Rangeland and Wildlife Management
- Genetic Mapping and Diversity in Plants and Animals
New South Wales Department of Primary Industries
2020-2022
AgResearch
2006-2014
Plant Industry
2006
Swedish University of Agricultural Sciences
2006
Umeå Plant Science Centre
2006
Plant Gene Expression Center
2000-2004
Noble Research Institute
1999-2000
Salk Institute for Biological Studies
1997-2000
Health First
2000
John Innes Centre
1993-1997
FLOWERING LOCUS T ( FT ), which acts in parallel with the meristem-identity gene LEAFY LFY ) to induce flowering of Arabidopsis , was isolated by activation tagging. Like partially downstream CONSTANS CO promotes response long days. Unlike many other floral regulators, deduced sequence protein does not suggest that it directly controls transcription or transcript processing. Instead, is similar TERMINAL FLOWER 1 (TFL1), an inhibitor also shares similarity membrane-associated mammalian proteins.
Activation tagging using T-DNA vectors that contain multimerized transcriptional enhancers from the cauliflower mosaic virus (CaMV) 35S gene has been applied to Arabidopsis plants. New activation-tagging confer resistance antibiotic kanamycin or herbicide glufosinate have used generate several tens of thousands transformed From these, over 30 dominant mutants with various phenotypes isolated. Analysis a subset shown overexpressed genes are almost always found immediately adjacent inserted...
Summary Two rapid and simple in planta transformation methods have been developed for the model legume Medicago truncatula . The first approach is based on a method of Arabidopsis thaliana involves infiltration flowering plants with suspension Agrobacterium second young seedlings In both cases proportion progeny infiltrated transformed. frequency ranges from 4.7 to 76% flower method, 2.9 27.6% seedling method. Both procedures resulted mixture independent transformants sibling transformants....
Abstract Hybridization may lead to introgression of genes among species. Introgression be bidirectional or unidirectional, depending on factors such as the demography hybridizing species, nature reproductive barriers between them. Previous microsatellite studies suggested diploid Betula nana (dwarf birch) and tetraploid B. pubescens (downy also pendula (silver in Britain. Here, we analyse these species using 51 237 variants restriction site‐associated ( RAD ) markers 194 individuals, called...
MFT (MOTHER OF FT AND TFL1) is a member of gene family that includes two important regulators, (FLOWERING LOCUS T) and TFL1 (TERMINAL FLOWER 1), in determination flowering time Arabidopsis. Although the functions are assigned family, roles other members largely unknown. Especially sequence homologous to both TFL1, which act as floral promoter an inhibitor, respectively, making it difficult predict role MFT. We performed genetic analyses understand its development. Constitutive expression led...
Abstract Seasonal control of flowering often involves leaf sensing daylength coupled to time measurement and generation transport florigenic signals the shoot apex. We show that transmitted in grass Lolium temulentum may include gibberellins (GAs) FLOWERING LOCUS T (FT) gene. Within 2 h starting a florally inductive long day (LD), expression 20-oxidase GA biosynthetic gene increases leaf; its product, GA20, then 5.7-fold versus short day; substrate, GA19, decreases equivalently; bioactive...
Coding sequences for two cysteine proteases were amplified from cDNA derived pea nodule mRNA using primers based on conserved regions of plant proteases. One the corresponded to a previously described protease gene, Cyp15a, expressed in shoots response dehydration (J.T. Jones and J.E. Mullet, Plant Mol. Biol. 28:1055-1065, 1995). Inside root nodule, situ hybridization revealed that this gene is strongly apical region more weakly uninfected cortex central infected tissue where nitrogen...
Abstract A 27-kD glycoprotein antigen recognized by monoclonal antibody MAC266 was purified from isolated symbiosomes derived pea (Pisum sativum) root nodules containing Rhizobium. The N-terminal amino acid sequence obtained, and the corresponding cDNA clone a polymerase chain reaction-based strategy. contained single open reading frame, gene termed PsNlec1. Phylogenetic analysis of 31 legume sequences showed that PsNlec1 protein is related to lectin family but belongs subgroup very...
The distribution of a lectinlike glycoprotein, PsNLEC-1, was examined with specific antiserum in nodule tissue from symbiotically defective pea mutant, Sprint2Fix¯(sym31), and the parent line, Sprint2. Immunostaining Western blots (immunoblots) revealed that, whereas wild-type nodules contained three antigenic isoforms homogenates mutant sym31 only one isoform, PsNLEC-1C. Fractionation studies indicated that PsNLEC-1C not associated symbiosomes either or (unlike other two nodules). Light...
Abstract The pea (Pisum sativum) nodule lectin gene PsNlec1 is a member of the legume family that strongly expressed in infected tissue. A full-length cDNA sequence was Escherichia coli and specific antiserum generated from purified protein. Immunoblotting material isolated symbiosomes revealed glycoprotein present two antigenic isoforms, PsNLEC-1A PsNLEC-1B. N-terminal isoform showed homology to an eight-amino acid propeptide previously identified B. In homogenates recognized additional...
Abstract Smart farming for extensive grazing systems includes applications linking environment and supply-chain, including metrics climate, soils, pastures, animals animal products to enhance management, optimization predictions. Technological developments remote monitoring in have varied their success remain limited uptake, include: In-field, fixed-device of livestock numbers, water, photosynthesis greenhouse gas emissions; body composition physiology assessments using devices fixed...
INTRODUCTION This article describes the analysis and quantification of Arabidopsis RNA by reverse transcriptase-polymerase chain reaction (RT-PCR). is first reverse-transcribed into cDNA, which then amplified competitive PCR using a mimic. Multiple PCRs are performed constant amount unknown cDNA varying amounts mimic; that is, template known concentration close in size GC content to quantified target contains same annealing sites for primers. Mimics usually designed produce products ~10%-20%...
Abstract Background Grasslands are the primary source of feed for grazing livestock, and as such, knowledge on how to best manage livestock grasslands, through use spatiotemporal modelling, will assist in long‐term management a valuable ecosystem resource. Methods This study was conducted over 14 months between March April 2017 Orange, NSW, Australia. The evaluated sheep behaviour relation presence pasture species, environment paddock structures, using random forest predict location under...