The effects of temperature, agitation and aeration on glycoprotein GP-1 production by Streptomyces kanasenisi ZX01 in bench-scale fermentors were systematically investigated. maximum final was achieved at an speed 200 rpm, rate 2.0 vvm temperature 30 °C. By using a dynamic gassing out method, the volumetric oxygen transfer coefficient (kLa) also studied. values logarithmic phase increased with increase (from 14.53 to 32.82 h-1) 13.21 22.43 h-1). In addition, successful scale-up from...

Plant diseases caused by phytopathogenic fungi and oomycetes pose a serious threat to ensuring crop yield quality. Finding novel fungicidal candidates based on natural products is one of the critical methods for developing effective environmentally friendly pesticides. In this study, series salicylaldehyde derivatives containing an α-methylene-γ-butyrolactone moiety were designed, synthesized, their activities evaluated.The bioassay studies indicated that compound C3 displayed excellent in...

To discover novel fungicides with unique structures, a series of α-methylene-γ-butyrolactone derivatives were designed and synthesized by incorporating flexible amide or hydrazide chain through active substructure splicing linker modification strategies. Bioassay assessments demonstrated that certain hydrazide-containing compounds have potent fungicidal efficacy. Notably, compound 7IIj exhibited broad-spectrum antifungal activity, EC50 values as low 0.179, 0.301, 0.647, 0.549, 0.789 mg/L...

On the basis of structure nicotlactone A (L1), a series novel α-methylene-γ-butyrolactone derivatives B1–B43 were designed and synthesized by simplification active fragment replacement strategies, their antiviral antifungal activities evaluated. The bioassay studies indicated that many target compounds possessed good to excellent activity against tobacco mosaic virus (TMV) some these exhibited specific Valsa mali Fusarium graminearum. Compound B32 best anti-TMV (inactivation effect, 88.9%;...

To extract and identify the metabolites of strain A217 as well its antifungal spectrum control effect on various plant pathogens. Strain was identified a Streptomyces sp. which most similar to lienomycini. An antimicrobial test indicated that inhibited several pathogenic fungi strong antibacterial such Phytophthora capsici, Botrytis cinerea, Sclerotinia sclerotiorum, Fusarium oxysporum, Pseudomonas syringae Xanthomonas campestris. in vivo tissue demonstrated fermentation broth exerted...

Natural products are a valuable resource for the discovery of novel crop protection agents. A series γ-butyrolactone derivatives, derived from simplification podophyllotoxin's structure, were synthesized and assessed their efficacy against tobacco mosaic virus (TMV). Several derivatives exhibited notable antiviral properties, with compound

Streptomyces kanasenisi ZX01 was found to produce a novel glycoprotein GP-1 previously, which secreted into medium and had significant activity against tobacco mosaic virus. However, the low production of by strain limited its further studies. In order improve yield GP-1, series statistical experimental design methods were applied optimize in this work. Millet chosen be optimal original for optimization. Soluble starch yeast extract identified as carbon nitrogen source using...

Phrymarolin II, a furofuran lignan isolated from Phryma leptostachya L., features 3,7-dioxabicyclo[3.3.0]octane skeleton. Herein, we report an alternative total synthesis of (±)-phrymarolin II (2), which was performed in 9 steps commercially available sesamol. The key the included zinc-mediated Barbier-type allylation and copper-catalyzed anomeric O-arylation. Our allowed analogues II. Most derivatives displayed good to excellent vivo activity against tobacco mosaic virus (TMV)....

Natural products have been a valuable source of efficient and low-risk pesticides. In this work, series novel sesamolin derivatives A0–A31 B0–B4 were designed synthesized via structural simplification furofuran lignan phrymarolin II, their antiviral antibacterial activities systematically evaluated. The bioassay results showed that compound A24 displayed remarkable inactivation activity against tobacco mosaic virus (TMV) with an EC50 value 130.4 μg/mL, which was superior to commercial...

Abstract The synthesis of the originally proposed structure (±)-nicotlactone A, a potent antiviral lignan with three continuous chiral centers, is reported in 5 steps from methyl acrylate. key included an In-catalyzed regioselective allylation and Mn-catalyzed Mukaiyama hydration reaction. Our synthetic strategy also enabled us to get other epimers investigate structure–activity relationship. NMR data synthesized compounds do not match that isolated sample, indicating nicotlactone A remains...

The γ-butyrolactone scaffold, commonly present in natural products and bioactive compounds, has played a crucial role the development of novel pesticides. In this study, series α-methylene-γ-butyrolactone derivatives containing diphenyl ether moiety were designed synthesized using scaffold splicing strategy. Bioassays revealed that several target compounds demonstrated potent fungicidal activities, particularly against Phytophthora capsici Valsa mali. Notably, compound B7 (EC50 = 0.809 mg/L)...