A5013006101- Coral and Marine Ecosystems Studies
- Telomeres, Telomerase, and Senescence
- Marine Sponges and Natural Products
- Aquaculture disease management and microbiota
- Plant Genetic and Mutation Studies
- Chromosomal and Genetic Variations
- Chemical Synthesis and Analysis
- Nutrition, Genetics, and Disease
- Plant Molecular Biology Research
- Epigenetics and DNA Methylation
- Animal Genetics and Reproduction
- Asymmetric Hydrogenation and Catalysis
- Peptidase Inhibition and Analysis
- Cancer therapeutics and mechanisms
- RNA Interference and Gene Delivery
- Marine Invertebrate Physiology and Ecology
- Marine and coastal plant biology
- Advanced biosensing and bioanalysis techniques
- Silicon Effects in Agriculture
- Marine and fisheries research
- Click Chemistry and Applications
- Biochemical and Molecular Research
- Cell death mechanisms and regulation
- Nanopore and Nanochannel Transport Studies
- PARP inhibition in cancer therapy
University of Hawaiʻi at Mānoa
2020-2024
University of Hawaii System
2020-2024
de Duve Institute
2017-2021
Leidos Biomedical Research Inc. (United States)
2017
Leidos (United States)
2017
Frederick National Laboratory for Cancer Research
1998-2017
Masaryk University
2011-2014
National Cancer Institute
1997-2013
Czech Academy of Sciences
2013
Center for Cancer Research
2013
Phenotypic plasticity is an important ecological and evolutionary response for organisms experiencing environmental change, but the ubiquity of this capacity within coral species across symbiont communities unknown. We exposed ten genotypes reef-building Montipora capitata with divergent to four thermal pre-exposure profiles quantified gene expression before stress testing 4 months later. Here we show two significantly enhance tolerance despite broadly different patterns substantial...
Abstract Reef‐building corals rely on intracellular algal symbionts to meet energetic demands. Increasing extreme weather driven by climate change often leads disruption of this symbiosis and coral death. Corals can better withstand stress after previous exposure sublethal conditions, but the mechanisms for resilience remain unclear. Here, we show that a three‐day thermal preconditioning increases tolerance acute heat through modulations in cell death pathways stony Pocillopora acuta . In...
Unlimited replicative potential is one of the hallmarks cancer cells. In melanoma, hTERT (telomerase reverse transcriptase) frequently overexpressed because activating mutations in its promoter, suggesting that telomerase necessary for melanoma development. We observed, however, a subset metastases and derived cell lines had no telomere maintenance mechanism. Early passages latter displayed long telomeres progressively shortened fused before death. propose that, during formation, oncogenic...
Epigenetic mechanisms are involved in regulation of crucial cellular processes eukaryotic organisms. Data on the epigenetic features plant telomeres and their were published mostly for Arabidopsis thaliana, which presence interstitial telomeric repeats (ITRs) may interfere with genuine most analyses. Here, we studied landscape transcription ITRs Nicotiana tabacum long no detectable ITRs, Ballantinia antipoda large blocks pericentromeric relatively short telomeres. Chromatin displayed...
Abstract Background Coral reefs house about 25% of marine biodiversity and are critical for the livelihood many communities by providing food, tourism revenue, protection from wave surge. These magnificent ecosystems under existential threat anthropogenic climate change. Whereas extensive ecological physiological studies have addressed coral response to environmental stress, high-quality reference genome data lacking these species. The latter issue hinders efforts understand genetic basis...
Caspases are important mediators of apoptotic cell death. Several cellular protein substrates caspases contain potential phosphorylation site(s) at the cleavage-site region, and some these sites have been verified to be phosphorylated. Since may affect substantially substrate susceptibility towards proteolysis, phosphorylated, non-phosphorylated substituted oligopeptides representing such cleavage were studied as 3, 7 8. Peptides containing phosphorylated serine residues P4 P1' positions...
Abstract Telomeres, nucleoprotein structures at the ends of linear eukaryotic chromosomes, are important for maintenance genomic stability. Telomeres were considered as typical heterochromatic regions, but in light recent results, this view should be reconsidered. Asymmetrically located cytosines plant telomeric DNA repeats may substrates a methyltransferase enzyme and indeed, it was shown that these methylated. Here, we analyse methylation length telomeres Arabidopsis thaliana mutants (met...
Iododeoxyuridine (IdUrd), a halogenated nucleoside analog, produced clinical responses when administered as radiosensitizer via continuous intravenous (c.i.v.) infusion over the course of radiotherapy. We conducted phase 0 trial 5-iodo-2-pyrimidinone-2'-deoxyribose (IPdR), an oral prodrug IdUrd, in patients with advanced malignancies to assess whether route was feasible alternative c.i.v. before embarking on large-scale trials. Plasma concentrations IPdR, and other metabolites were measured...
Global coral reef decline is largely driven by the breakdown of coral-algal symbiosis during temperature stress. Corals can acclimatize to higher temperatures, but cellular processes underlying this ability are poorly understood. We show that preconditioning-based improvements in thermal tolerance Pocillopora acuta accompanied increases host glutathione reductase (GR) activity and gene expression, which prevents DNA damage. A strong correlation between GR BI-1 expressions heat-stressed...
Two methods were devised to conjugate PEG alsterpaullone (NSC 705701) via the N of indole ring portion molecule. In first approach, activation was accomplished by reaction with p-nitrophenyl chloroformate produce a reactive carbamate that then condensed mono blocked diamine form urea bond followed deblocking and conjugation PEG. The second route used anion produced bond. Both compounds highly water soluble, stable in buffer, released vitro vivo. Studies conducted mice investigate influence...
Vimentin, a cellular substrate of HIV type 1 (HIV‐1) proteinase, contains protein kinase C (PKC) phosphorylation site at one its cleavage sites. Peptides representing this were synthesized in P2 Ser‐phosphorylated and nonphosphorylated forms. While the peptide was fairly good enzyme, prevented hydrolysis. Phosphorylation human recombinant vimentin by PKC processing within head domain, where occurred. Oligopeptides naturally occurring sites C‐terminus Rous sarcoma virus integrase assayed as...
A gradient reversed-phase high performance liquid chromatographic method was developed for determining NSC 737664 (2-[(2R)-2-methylpyrrolidin-2-yl]-1H-benzimidazole-4-carboxamide; ABT-888) in human plasma and urine. Chromatographic separation used a mobile phase composed of 0.1% formic acid water acetonitrile, C18 column (150 mm × 4.6 mm, 5µ). Quantitation performed using UV detection at 300 nm. peak identity confirmed positive-ion electrospray ionization mass spectrometry. The shown to be...
Abstract Climate change is rapidly pushing coral reefs towards extinction. Efforts to restore and protect these ecosystems using resilient corals are increasing, but our understanding of cellular mechanisms thermal resilience trade-offs remains limited. Here, we demonstrate downregulation pa-BAK slows bleaching stabilizes the symbiosis during acute heat stress in Pocillopora acuta . Counterintuitively, oxidative DNA damage – a hallmark response was prevented with such “forced” stability,...
<title>Abstract</title> Coral bleaching is one of the greatest climate-driven threats to persistence tropical reef ecosystems. This necessitates identification attributes associated with coral resistance and resilience thermal stress both within between generations. Here, we used metabolomics demonstrate that biochemical signatures heat-induced <italic>Montipora capitata</italic> are passed There were metabolomic parental phenotype in sperm, eggs, embryos, larvae, subsequent juvenile corals...
Segmentation of the gastric cavity is a synapomorphic trait cnidarians class Anthozoa (corals and sea anemones), with different clades forming distinct numbers segments. In starlet anemone Nematostella vectensis , for example, eight bilaterally positioned segments are generated by action group Hox-Gbx genes in developing larval endo-mesoderm. Still, given range segment observed anthozoans, it remains unclear whether this module evolutionarily conserved how might be deployed to generate Here,...
Segmentation of the gastric cavity is a synapomorphic trait cnidarians class Anthozoa (corals and sea anemones), with different clades forming distinct numbers segments. In starlet anemone Nematostella vectensis , for example, eight bilaterally positioned segments are generated by action group Hox-Gbx genes in developing larval endo-mesoderm. Still, given range segment observed anthozoans, it remains unclear whether this module evolutionarily conserved how might be deployed to generate Here,...