A5087856084- Marine Invertebrate Physiology and Ecology
- Marine Ecology and Invasive Species
- Coral and Marine Ecosystems Studies
- Planarian Biology and Electrostimulation
- Cephalopods and Marine Biology
- Exercise and Physiological Responses
- Muscle Physiology and Disorders
- Identification and Quantification in Food
- Tissue Engineering and Regenerative Medicine
- NF-κB Signaling Pathways
- Marine Sponges and Natural Products
- Photoreceptor and optogenetics research
- Protist diversity and phylogeny
- Developmental Biology and Gene Regulation
- Genomics and Phylogenetic Studies
Stowers Institute for Medical Research
2020-2024
MRIGlobal
2023
Northwestern University
2015-2018
Mechanisms determining final organ size are poorly understood. Animals undergoing regeneration or ongoing adult growth likely require sustained and robust mechanisms to achieve maintain appropriate sizes. Planarians, well known for their ability undergo whole-body due pluripotent stem cells of the neoblast population, can reversibly scale body-size over an order magnitude by controlling cell number. Using quantitative analysis, we show that after injury planarians perfectly restore...
Abstract There is currently little information about the evolution of gene clusters, genome architectures and karyotypes in early branching animals. Slowly evolving anthozoan cnidarians can be particularly informative these features. Here we report chromosome-level assemblies two related anthozoans, sea anemones Nematostella vectensis Scolanthus callimorphus . We find a robust set 15 chromosomes with clear one-to-one correspondence between species. Both genomes show chromosomal conservation,...
Abstract Draft genome sequences of non-bilaterian species have provided important insights into the evolution metazoan gene repertoire. However, there is little information about clusters, architectures and karyotypes during animal evolution. In this regard, slowly evolving anthozoan Cnidaria, sister group Bilateria, are particularly informative. Here we report chromosome-level assemblies two related cnidarians, sea anemones Nematostella vectensis Scolanthus callimorphus . We find a robust...
Most animals undergo homeostatic tissue maintenance, yet those capable of robust regeneration in adulthood use mechanisms significantly overlapping with homeostasis. Here we show planarians that modulations to body-wide patterning systems shift the target site for eye while still enabling homeostasis eyes outside this region. The uncoupling and regeneration, which can occur during normal positional rescaling after axis truncation, is not due altered injury signaling or stem cell activity,...
Bilaterian animals have evolved complex sensory organs comprised of distinct cell types that function coordinately to sense the environment. Each unit has a defined architecture built from component types, including cells, non-sensory support and dedicated neurons. Whether this characteristic cellular composition is present in non-bilaterian unknown. Here, we interrogate type gene regulatory networks controlling development larval apical organ sea anemone Nematostella vectensis. Using single...
Abstract Draft genome sequences of non-bilaterian species have provided important insights into the evolution metazoan gene repertoire. However, there is little information about clusters, architectures and karyotypes during animal evolution. Here we report chromosome-level assemblies two related anthozoan cnidarians, sea anemones, Nematostella vectensis Scolanthus callimorphus . We find a robust set 15 chromosomes with clear one-to-one correspondence between species. show that, in contrast...
Abstract With a surprisingly complex genome and an ever-expanding genetic toolkit, the sea anemone Nematostella vectensis has become powerful model system for study of both development whole-body regeneration. Here we provide most current protocols short-hairpin RNA (shRNA)-mediated gene knockdown CRISPR/Cas9-targeted mutagenesis in this . We further show that simple Klenow reaction followed by vitro transcription allows production gene-specific shRNAs single guide RNAs (sgRNAs) fast,...
Abstract Bilaterian animals have evolved complex sensory organs comprised of distinct cell types that function coordinately to sense the environment. Each unit has a defined architecture built from component types, including cells, non-sensory support and dedicated neurons. Whether this characteristic cellular composition is present in non-bilaterian unknown. Here, we interrogate type gene regulatory networks controlling development larval apical organ sea anemone Nematostella vectensis ....
Segmentation of the gastric cavity is a synapomorphic trait cnidarians class Anthozoa (corals and sea anemones), with different clades forming distinct numbers segments. In starlet anemone Nematostella vectensis, for example, eight bilaterally positioned segments are generated by action group Hox-Gbx genes in developing larval endo-mesoderm. Still, given range segment observed anthozoans, it remains unclear whether this module evolutionarily conserved how might be deployed to generate Here,...
Segmentation of the gastric cavity is a synapomorphic trait cnidarians class Anthozoa (corals and sea anemones), with different clades forming distinct numbers segments. In starlet anemone Nematostella vectensis , for example, eight bilaterally positioned segments are generated by action group Hox-Gbx genes in developing larval endo-mesoderm. Still, given range segment observed anthozoans, it remains unclear whether this module evolutionarily conserved how might be deployed to generate Here,...
Segmentation of the gastric cavity is a synapomorphic trait cnidarians class Anthozoa (corals and sea anemones), with different clades forming distinct numbers segments. In starlet anemone Nematostella vectensis , for example, eight bilaterally positioned segments are generated by action group Hox-Gbx genes in developing larval endo-mesoderm. Still, given range segment observed anthozoans, it remains unclear whether this module evolutionarily conserved how might be deployed to generate Here,...
Abstract Most animals undergo homeostatic tissue maintenance, yet those capable of robust regeneration in adulthood use mechanisms significantly overlapping with homeostasis. Here we show planarians that modulations to body-wide patterning systems shift the target site for eye while still enabling homeostasis eyes outside this region. The uncoupling and regeneration, which can occur during normal positional rescaling after axis truncation, is not due altered injury signaling or stem cell...