A5045223106- CRISPR and Genetic Engineering
- Neurobiology and Insect Physiology Research
- Evolution and Genetic Dynamics
- Aquatic Ecosystems and Phytoplankton Dynamics
- Physiological and biochemical adaptations
- Biocrusts and Microbial Ecology
- Crustacean biology and ecology
- Water Quality and Pollution Assessment
- Plant Stress Responses and Tolerance
- RNA modifications and cancer
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Photoreceptor and optogenetics research
- Aquaculture Nutrition and Growth
- RNA Research and Splicing
- Insect Utilization and Effects
- Cancer-related molecular mechanisms research
- Genetics, Aging, and Longevity in Model Organisms
- Water Quality Monitoring Technologies
- Environmental Toxicology and Ecotoxicology
- Insect symbiosis and bacterial influences
Osaka University
2018-2025
Long noncoding RNAs (lncRNAs) are vastly transcribed and extensively studied but lncRNAs overlapping with the sense orientation of mRNA have been poorly studied. We analyzed lncRNA DAPALR 5´ UTR Doublesex1 ( Dsx1 ), male determining gene in Daphnia magna . By affinity purification, we identified an RNA binding protein, Shep as a protein. also binds to by recognizing sequence suppresses translation mRNA. In vitro vivo analyses indicated that increased efficiency sequestration Shep. This...
The cladoceran crustacean Daphnia exhibits phenotypic plasticity, a phenomenon that leads to diverse phenotypes from one genome. Alternative usage of gene isoforms has been considered key regulation mechanism for controlling different phenotypes. However, understand the plasticity Daphnia, have not comprehensively analyzed. Here we identified 25,654 transcripts derived 9710 genes expressed during environmental sex determination magna using long-read RNA-Seq with PacBio Iso-Seq. We found...
The ecdysteroid and sesquiterpenoid pathways control growth, developmental transition, embryogenesis in insects. However, the function of orthologous genes cross-talk between both remain largely uncharacterized non-insect arthropods. Spook (Spo) Juvenile hormone acid o-methyltransferase (Jhamt) have been suggested to as rate-limiting factors biosynthesis, respectively, In this study, we report on functions Spo Jhamt them embryos branchiopod crustacean Daphnia magna. expression was activated...
Salinization poses an increasing problem worldwide, threatening freshwater organisms and raising questions about their ability to adapt. We explored the mechanisms enabling a planktonic crustacean tolerate elevated salinity. By gradually water salinity in clonal cultures from 185 Daphnia magna populations, we showed that salt tolerance strongly correlates with native habitat salinity, indicating local adaptation. A genome-wide association study (GWAS) further revealed major effect of...
Abstract Salinization poses an increasing problem worldwide, threatening freshwater organisms and raising questions about ability to adapt. We explore the mechanisms enabling a planktonic crustacean tolerate elevated salinity. By gradually water salinity in clonal cultures from 185 Daphnia magna populations, we showed that salt tolerance strongly correlates with native habitat salinity, indicating local adaptation. A GWAS revealed major effect of Alpha,alpha-trehalose-phosphate synthase (...
The crustacean Daphnia magna is an emerging model for ecological and toxicological genomics. However, the lack of methods spatial temporal control gene expression has impaired elucidation molecular mechanisms underlying responses to environments in vivo. Here we report local activation hsp70 promoter-driven cassette D. by infrared laser-evoked operator (IR-LEGO), a method heating target cells with irradiation. We identified heat-inducible promoter upstream hsp70-A gene. Using this promoter,...
Abstract In the crustacean Daphnia magna , studying homology-directed repair (HDR) is important to understand genome maintenance during parthenogenesis, effects of environmental toxicants on genome, and improvement HDR-mediated editing. Here we developed a transgenic D. that expresses green fluorescence protein (GFP) upon HDR occurrence. We utilized previously established reporter plasmid named DR-GFP has mutated eGFP gene ( SceGFP ) tandemly located donor GFP fragment iGFP ). Upon...
In sex determination of the crustacean Daphnia magna , male-specific expression DM-domain transcription factor Doublesex1 (Dsx1) orchestrates male developmental program triggered by environmental stimuli. We previously identified CELF1 ortholog as a candidate proteins associated with 5’ UTR Dsx1α isoform. Here we report CELF1-dependent suppression Dsx1 in D . During embryogenesis, was not sexually dimorphic. Silencing led to activation both female and embryos. Overexpression embryos resulted...