A5040825965- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- SARS-CoV-2 and COVID-19 Research
- Modular Robots and Swarm Intelligence
- Monoclonal and Polyclonal Antibodies Research
- Immune cells in cancer
- Supramolecular Self-Assembly in Materials
- Dendrimers and Hyperbranched Polymers
- Bacteriophages and microbial interactions
- Peptidase Inhibition and Analysis
- Click Chemistry and Applications
- Single-cell and spatial transcriptomics
Aarhus University
2022-2024
Central Denmark Region
2023
Abstract The compaction and organization of genomic DNA is a central mechanism in eukaryotic cells, but engineered architectural control over double‐stranded (dsDNA) notably challenging. Here, long dsDNA templates are folded into designed shapes via triplex‐mediated self‐assembly. Triplex‐forming oligonucleotides (TFOs) bind purines normal or reverse Hoogsteen interactions. In the triplex origami methodology, these non‐canonical interactions programmed to compact (linear plasmid)...
Abstract DNA nanostructures have considerable biomedical potential as intracellular delivery vehicles they are highly homogeneous and can be functionalized with high spatial resolution. However, challenges like instability under physiological conditions, limited cellular uptake, lysosomal degradation limit their use. This paper presents a bio‐reducible, cationic polymer poly(cystaminebisacrylamide‐1,6‐diaminohexane) (PCD) reversible origami protector. PCD displays stronger affinity than...
Arginine is one of the less commonly targeted amino acids in protein bioconjugation, despite its unique reactivity and abundance on surface proteins. In this work, a molecule containing diketopinic acid an azide handle was developed for chemo-selective bioconjugation to arginine. This compound proved be efficient IgG1 IgG4 antibodies, achieving mono- double-label conversion rates 37-44 12-30%, respectively. Mass spectrometry analysis confirmed antibody modification at two conserved regions....
DNA Nanotechnology nanotechnology, so far, has almost exclusively relied on Watson–Crick base pairing. In article number 2302497, Minke A. D. Nijenhuis, Kurt V. Gothelf, and co-workers introduce a triplex origami methodology. They demonstrate that DNA, formed by Hoogsteen interactions, also possesses remarkable potential for nanoscale engineering. Triplex enables unprecedented large-scale architectural control over double-stranded templates, folding compacting them into sheets, bundles, even...
The folding of double-stranded DNA around histones is a central mechanism in eukaryotic cells for compacting the genetic information into chromosomes. Very few artificial methods are available controlling shape dsDNA at any level, whereas several have been developed to efficiently organize single-stranded and RNA variety well-defined nanostructures by programmed self-assembly , . Here, we show how long sequences can be spatially organized triplex forming oligonucleotides (TFOs), which bridge...
The folding of double-stranded DNA around histones is a central mechanism in eukaryotic cells for compacting the genetic information into chromosomes. Very few artificial methods are available controlling shape dsDNA at any level, whereas several have been developed to efficiently organize single-stranded and RNA variety well-defined nanostructures by programmed self-assembly. Here, we show how long sequences can be spatially organized triplex-forming oligonucleotides (TFOs), which bridge...