A5014224455- Parasitic Diseases Research and Treatment
- Parasite Biology and Host Interactions
- Parasites and Host Interactions
- Insects and Parasite Interactions
- Parasitic infections in humans and animals
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Parasitic Infections and Diagnostics
- Inflammatory mediators and NSAID effects
- Immune Response and Inflammation
- Immunotherapy and Immune Responses
- Insect Utilization and Effects
- Cancer Research and Treatments
- Toxoplasma gondii Research Studies
- Neuroendocrine regulation and behavior
- Consumer Attitudes and Food Labeling
- T-cell and B-cell Immunology
- Nitric Oxide and Endothelin Effects
- Congenital Anomalies and Fetal Surgery
- Infant Nutrition and Health
- Antifungal resistance and susceptibility
- Mast cells and histamine
- Planarian Biology and Electrostimulation
- Tissue Engineering and Regenerative Medicine
- Cancer Immunotherapy and Biomarkers
- Identification and Quantification in Food
University of Belgrade
2008-2025
Institute for the Application of Nuclear Energy
2012-2023
University of Veterinary Medicine
2012
Military Medical Academy
2005-2008
Many parasites (protozoa and helminths) can be transmitted through food lead to infections with high morbidity, as well disease outbreaks. Although the importance of foodborne (FBP) is recognised by many sectors industry, standardized analytical methods validation procedures for testing FBP are lacking. Scope approach:Current detection FBP, their validation, critically reviewed, focusing on priority in Europe: helminths Echinococcus multilocularis, granulosus, Taenia saginata, Trichinella...
Extracellular vesicles (EVs) represent a newly discovered but universal communication tool between cells or organisms. However, few data exist on nematode EVs and none for Trichinella spiralis. Here, we aimed to investigate whether T spiralis muscle larvae produce EVs, they carry immunomodulatory proteins have role in immunomodulation as component of excretory-secretory products (ES L1).EVs were enriched from conditioned medium larvae. Transmission electron microscopy images showed be 30-80...
Purpose: Trichinella spiralis has evolved complex immunomodulatory mechanisms mediated by excretory-secretory products (ESL1) that enable its survival in the host. Consequently, ESL1 antigens display excellent potential for treating autoimmune diseases such as multiple sclerosis (MS). However, whether timely controlled delivery of vivo, natural infections, could enhance therapeutic MS is still unknown. Methods: To test this, we encapsulated into biodegradable poly (lactide-co-glycolic) acid...
SUMMARY Pathogen‐derived products have the capacity to induce maturation of bone marrow‐derived dendritic cells (BMDCs) into populations effectors that polarize Th toward Th1 or Th2 phenotype via different mechanisms. Since those mechanisms are not entirely clear for helminths, and almost completely unknown Trichinella spiralis (TS), we started an investigation effects TS antigens (four isolated from all three life‐cycle stages parasite) on BMDCs their potential present antigens. The...
Abstract In Europe, Serbia ranks among countries with a high prevalence of Trichinella infection in pigs, which continues to be serious human health problem. While some Balkan countries, more than one species/genotype has been described both the sylvatic and domestic cycles, these data are lacking for Serbia. To date, only few Serbian isolates have genetically specified, all were classified as T. spiralis. Although transmission from pigs wildlife could assumed, neither status nor species...
ENWEndNote BIBJabRef, Mendeley RISPapers, Reference Manager, RefWorks, Zotero AMA Vasilev S, Ilic N, Gruden-Movsesijan A, Vasilijic Bosic M, Sofronic-Milosavljevic L. Experimental immunologyNecrosis and apoptosis in Trichinella spiralis-mediated tumour reduction. Central European Journal of Immunology. 2015;40(1):42-53. doi:10.5114/ceji.2015.50832. APA Vasilev, S., Ilic, N., Gruden-Movsesijan, A., Vasilijic, Bosic, M., & Sofronic-Milosavljevic, (2015). Immunology, 40(1), 42-53....
In Serbia, modern pork production systems with implemented control measures, including the detection of Trichinella larvae in meat (ISO18743), have eliminated farmed from pigs slaughtered at abattoirs as a source trichinellosis. Epidemiological data 2011 to 2020 indicate that number human cases and infected domestic has decreased significantly. Over years, was most frequent infection. Cases generally occurred small family outbreaks, infection linked consumption raw or undercooked backyard...
Abstract Infection with parasites from the Trichinella genus occurs in many vertebrates but disease only humans (trichinellosis). Humans are infected after consumption of raw or undercooked meat wild domestic animals (usually swine horses). Using monoclonal antibody (mAb) 7C2C5, specific for an epitope unique to muscle larvae , we have developed a competitive enzyme-linked immunosorbent assay (c-ELISA) that enables rapid detection -specific antibodies sera originating two different host...
Neutrophils are a part of the immune system, and they involved in host defence against microorganisms. Neutrophil granulocytes have shortest lifespan among leukocytes, which can be modulated by cytokines pharmacological agents. The effect aspirin on apoptosis inflammatory has not been studied detail yet, therefore was chosen subject this study. Inflammatory isolated from polyvinyl sponges implanted under skin Albino Oxford (AO) rats. cells that were 20 hours later more than 95% neutrophil...
Introduction. Inter-laboratory comparative testing must be conducted under the direction of National Reference Laboratory for Trichinella in each Member State EU with aim to ensure quality approved digestion method and evaluate competence laboratories detection. For first time Serbia, Trichinellosis (NRLT INEP) organized such external assessment (EQA) detection larvae meat by Magnetic Stirrer Method (MSM). The this study was enable interested take part EQA assess their MSM performance....
Abstract Trichinellosis in humans is most often caused by the parasite Trichinella spiralis . The clinical course of disease diverse and symptoms can vary from mild to severe. Symptoms usually disappear within a few months, but encysted larvae muscles cause myalgia weakness that last for years. However, existence chronic trichinellosis as still debatable. This study presents results obtained at National Reference Laboratory – INEP, Serbia. was conducted assess immunoserological, biochemical...
Food can be an important route for transmission of parasites to humans. Compared other foodborne pathogens in Serbia, (or potentially foodborne) do not get the attention they undoubtedly deserve. The aim this article is give overview most parasitic that transmitted by food, and cause disease humans: Echinococcus, Trichinella, Taenia solium Toxoplasma gondii. For each these pathogens, severity human diseases cause, incidence, mortality case fatality rate among humans Serbia as well their...
Apoptosis of neutrophil granulocytes is a critical event in the resolution inflammation. Neutrophils have short lifespan which can be modulated by aspirin. In this work we studied effect nitroaspirin (NCX4040) on apoptosis inflammatory granulocytes. This nitro-aspirin has been synthesized attempt to reduce side effects aspirin gastrointestinal tract. Inflammatory isolated from polyvinyl sponges implanted under skin Albino Oxford (AO) rats. cells that were 20 hours later about 95% The...
On the basis of performed analyses factors that contributed infected meat reach food chain, we have found out infection occurred after consuming inspected by method collective samples artificial digestion using a magnetic stirrer (MM). In this work there are presented assay results which show how modifications method, on level final sedimentation, influence reliability Trichinella larvas detection in samples. It has been shown use inadequate laboratory containers for larva collecting...
Trichinellosis, a mandatory reportable disease, remains persistent zoonosis in Serbia due to regulatory violations, inadequate pork inspection, and insufficient awareness of health risks. Nonetheless, epidemiological data from the past decade (2014-2023) show significant downward trend Trichinella infection among population, with number cases last five years being three times lower than previous years. Over this ten-year period (except 2021) there have been 489 trichinellosis cases. Among...
In 2021, the Serbian National Reference Laboratory for Trichinellosis, Institute Application of Nuclear Energy, organized external quality assessment (EQA 2021) detection Trichinella larvae presence in meat by magnetic stirrer method (MSM). The aims this study were to examine performance accredited laboratories over time and compare participants' performance. EQA 2021 was according ISO 17043, test panel consisted three balls, two which spiked with four spiralis L1 larvae. Evaluation...
nema
An outbreak of trichinellosis occurred in Stari Banovci, a settlement the municipality Stara Pazova, Srem, Republic Serbia, March-April 2019. A total 28 persons were exposed and was confirmed 24 them. This involved members eight families, their relatives friends. The infection, due to Trichinella spiralis (Owen, 1835), associated with consumption pork sausages procured local butcher's shop. trace-back study revealed that meat originated from swine raised on small farm belonging owner shop,...
Apoptosis of neutrophil granulocytes is a critical event in the resolution inflammation. Neutrophils have short lifespan which can be modulated by aspirin. In this work we studied effect nitroaspirin (NCX4016) on apoptosis inflammatory granulocytes. This aspirin analogue has been synthesized to reduce side effects gastrointestinal tract. Inflammatory isolated from polyvinyl sponges implanted under skin Albino Oxford (AO) rats. cells that were 20 hours later about 95% The cultivated 24h with...