In this study, the application of Raman spectroscopy to simultaneous quantitative determination glucose, glutamine, lactate, ammonia, glutamate, total cell density (TCD), and viable (VCD) in a CHO fed-batch process was demonstrated situ 3 L 15 bioreactors. Spectral preprocessing partial least squares (PLS) regression were used correlate spectral data with off-line reference data. Separate PLS calibration models developed for each analyte at laboratory bioreactor scale before assessing its...

The use of laser light scattering technology, as applied by the Focused Beam Reflectance Measurement (FBRM) probe from Lasentec, for in-line monitoring particle size was investigated. In particular, effects position and orientation were studied a dilute agitated aqueous particulate suspension known distribution in number vessels. all cases, normalised chord length recorded with FBRM system consistent theoretically predicted distribution. However, total counts measured function both solids...

A continuously operated single stage mixed-suspension, mixed-product-removal (MSMPR) crystallizer using intermittent withdrawal via a dip pipe with combined pressure/vacuum was successfully developed for the manufacture of active pharmaceutical ingredients. Approximately 5.8% total operating volume intermittently removed at high velocity vacuum. The transfer line also periodically purged nitrogen to ensure complete removal residual solids. In situ process analytical technologies (focused...

Immobilization of pollutant-degrading microorganisms on oxygen-permeable membranes provides a novel method increasing the oxidation capacity wastewater treatment bioreactors. Oxygen mass transfer characteristics during continuous-flow steady-state experiments were investigated for biofilms supported tubular silicone membranes. An analysis oxygen transport and reaction using an established mathematical model dual-substrate limitation experimental results reported. In thick biofilms, active...

An investigation of the feasibility in situ monitoring polymorphic transitions is presented. Focused beam reflectance measurement (FBRM) and particle vision (PVM) are used to study changes crystal population morphology during transition δ-polymorph d-mannitol thermodynamically stable β-form. The transformation confirmed via off-line X-ray powder diffraction (XRPD). Nucleation growth β-form was detected measured by FBRM/PVM when δ-form added a saturated solution d-mannitol. Raman spectroscopy...

Nucleation and growth kinetics of benzoic acid crystallized by cooling from 1.5:1 (w/w) water/ethanol solutions were determined using a 500 mL continuous mixed suspension, product removal (MSMPR) crystallizer mathematical model the MSMPR process. The developed solves population, moment, mass balance relationships with kinetic expressions for system is coupled nonlinear optimization routine parameter estimation. Comprehensive experimental data fitting estimation was obtained varying operating...

In this work, a diffusion-theory-based model has been devised to simulate dissolution kinetics of poorly water-soluble drug, ibuprofen. The was developed from the Noyes–Whitney equation in which rate term is function remaining particulate surface area and concentration gradient across boundary layer. Other parameters include initial particle size, diffusion coefficient, material density, layer thickness. It useful for predicting nonsink circumstances under pure API polydisperse powders are...

The application of in situ FBRM and ATR-FTIR monitoring a polymorphic transformation solution d-mannitol is introduced. isothermal growth the stable β-form supersaturated initially monitored. This has needlelike habit, it noted that dominant chord length function width these needles. enables better understanding change distribution over time for growth. It also provides means to successfully track dynamics during from metastable δ-form. Monitoring and, more importantly, crystal count data...

The use of in situ tools to monitor the transformation a polymorphic material has potential provide unique information about mechanism and rate polymorphs. In this paper, solution mediated between α β form p-aminobenzoic acid (PABA) was investigated detail. Solubility PABA pure ethanol also reported for first time, allowing accurate determination transition temperature 13.8 °C. For experiments, Raman spectroscopy Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) were used...

A Monod kinetic model, logistic equation and statistical regression model were developed for a Chinese hamster ovary cell bioprocess operated under three different modes of operation (batch, bolus fed-batch, continuous fed-batch) grown on two bioreactor scales (3 L bench-top 15 pilot-scale). The was all study predicted density, glucose glutamine, lactate, ammonia concentrations well the bioprocess. However, it computationally demanding due to large number parameters necessary produce good...

The effect of liquid flow velocity on biofilm development in a membrane-aerated reactor was investigated both by mathematical modeling and experiment, using Vibrio natriegens as test organism acetate carbon substrate. It shown that influenced mass transfer the diffusion boundary layer, biomass detachment rate from biofilm, maximum thickness attained. Values overall coefficient tracer through membrane were to be identical during different experiments at thickness. Comparison results with...

d-Mannitol is a typical polymorphic crystalline compound. In this paper, transformation from the α to β form of mannitol monitored by in situ Raman spectroscopy, focused beam reflectance measurement (FBRM) and particle vision (PVM). The standard spectra two polymorphs were determined characteristic peaks for different chosen track process. By combining with FBRM PVM, relationships between fine particles metastable dissolution also coarse stable crystallization could be defined....